Canine Circovirus Suppresses the Type I Interferon Response and Protein Expression but Promotes CPV-2 Replication
Canine circovirus (CanineCV) is an emerging virus in canines. Since the first strain of CanineCV was reported in 2012, CanineCV infection has shown a trend toward becoming a global epidemic. CanineCV infection often occurs with coinfection with other pathogens that may aggravate the symptoms of dise...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/12/6382 |
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| author | Xiangqi Hao Yanchao Li Hui Chen Bo Chen Ruohan Liu Yidan Wu Xiangyu Xiao Pei Zhou Shoujun Li |
| author_facet | Xiangqi Hao Yanchao Li Hui Chen Bo Chen Ruohan Liu Yidan Wu Xiangyu Xiao Pei Zhou Shoujun Li |
| author_sort | Xiangqi Hao |
| collection | DOAJ |
| description | Canine circovirus (CanineCV) is an emerging virus in canines. Since the first strain of CanineCV was reported in 2012, CanineCV infection has shown a trend toward becoming a global epidemic. CanineCV infection often occurs with coinfection with other pathogens that may aggravate the symptoms of disease in affected dogs. Currently, CanineCV has not been successfully isolated by laboratories, resulting in a lack of clarity regarding its physicochemical properties, replication process, and pathogenic characteristics. To address this knowledge gap, the following results were obtained in this study. First, a CanineCV strain was rescued in F81 cells using infectious clone plasmids. Second, the Rep protein produced by the viral packaging rescue process was found to be associated with cytopathic effects. Additionally, the Rep protein and CanineCV inhibited the activation of the type I interferon (IFN-I) promoter, blocking subsequent expression of interferon-stimulated genes (ISGs). Furthermore, Rep was found to broadly inhibit host protein expression. We speculate that in CanineCV and canine parvovirus type 2 (CPV-2) coinfection cases, CanineCV promotes CPV-2 replication by inducing immunosuppression, which may increase the severity of clinical symptoms. |
| first_indexed | 2024-03-09T23:36:41Z |
| format | Article |
| id | doaj.art-138896c961594ae49493229c2d9b2fb8 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:36:41Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-138896c961594ae49493229c2d9b2fb82023-11-23T16:59:43ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-06-012312638210.3390/ijms23126382Canine Circovirus Suppresses the Type I Interferon Response and Protein Expression but Promotes CPV-2 ReplicationXiangqi Hao0Yanchao Li1Hui Chen2Bo Chen3Ruohan Liu4Yidan Wu5Xiangyu Xiao6Pei Zhou7Shoujun Li8College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaCanine circovirus (CanineCV) is an emerging virus in canines. Since the first strain of CanineCV was reported in 2012, CanineCV infection has shown a trend toward becoming a global epidemic. CanineCV infection often occurs with coinfection with other pathogens that may aggravate the symptoms of disease in affected dogs. Currently, CanineCV has not been successfully isolated by laboratories, resulting in a lack of clarity regarding its physicochemical properties, replication process, and pathogenic characteristics. To address this knowledge gap, the following results were obtained in this study. First, a CanineCV strain was rescued in F81 cells using infectious clone plasmids. Second, the Rep protein produced by the viral packaging rescue process was found to be associated with cytopathic effects. Additionally, the Rep protein and CanineCV inhibited the activation of the type I interferon (IFN-I) promoter, blocking subsequent expression of interferon-stimulated genes (ISGs). Furthermore, Rep was found to broadly inhibit host protein expression. We speculate that in CanineCV and canine parvovirus type 2 (CPV-2) coinfection cases, CanineCV promotes CPV-2 replication by inducing immunosuppression, which may increase the severity of clinical symptoms.https://www.mdpi.com/1422-0067/23/12/6382canine circoviruscoinfectioninterferon responseprotein expression inhibitioncanine parvovirus |
| spellingShingle | Xiangqi Hao Yanchao Li Hui Chen Bo Chen Ruohan Liu Yidan Wu Xiangyu Xiao Pei Zhou Shoujun Li Canine Circovirus Suppresses the Type I Interferon Response and Protein Expression but Promotes CPV-2 Replication International Journal of Molecular Sciences canine circovirus coinfection interferon response protein expression inhibition canine parvovirus |
| title | Canine Circovirus Suppresses the Type I Interferon Response and Protein Expression but Promotes CPV-2 Replication |
| title_full | Canine Circovirus Suppresses the Type I Interferon Response and Protein Expression but Promotes CPV-2 Replication |
| title_fullStr | Canine Circovirus Suppresses the Type I Interferon Response and Protein Expression but Promotes CPV-2 Replication |
| title_full_unstemmed | Canine Circovirus Suppresses the Type I Interferon Response and Protein Expression but Promotes CPV-2 Replication |
| title_short | Canine Circovirus Suppresses the Type I Interferon Response and Protein Expression but Promotes CPV-2 Replication |
| title_sort | canine circovirus suppresses the type i interferon response and protein expression but promotes cpv 2 replication |
| topic | canine circovirus coinfection interferon response protein expression inhibition canine parvovirus |
| url | https://www.mdpi.com/1422-0067/23/12/6382 |
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