Sulfated <i>Undaria pinnatifida</i> Polysaccharide Promotes Endocytosis of Nano-Calcium Oxalate Dihydrate by Repairing Subcellular Organelles in HK-2 Cells
The clinical manifestation of primary hyperoxaluria includes hyperoxaluria and recurrent urinary calculi. In this study, an oxidative damage model was constructed based on oxalate damage to the human renal proximal tubular epithelial cells (HK-2), and a comparative study was carried out on four diff...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-04-01
|
| Series: | Antioxidants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3921/12/5/1015 |
| _version_ | 1797601306040336384 |
|---|---|
| author | Xue-Wu Chen Yu-Yun Zheng Jian-Ming Ouyang |
| author_facet | Xue-Wu Chen Yu-Yun Zheng Jian-Ming Ouyang |
| author_sort | Xue-Wu Chen |
| collection | DOAJ |
| description | The clinical manifestation of primary hyperoxaluria includes hyperoxaluria and recurrent urinary calculi. In this study, an oxidative damage model was constructed based on oxalate damage to the human renal proximal tubular epithelial cells (HK-2), and a comparative study was carried out on four different sulfated levels of <i>Undaria pinnatifida</i> polysaccharides (UPP0, UPP1, UPP2, and UPP3 with sulfate group [–OSO<sub>3</sub><sup>−</sup>] contents of 1.59%, 6.03%, 20.83%, and 36.39%, respectively) on the repair of oxidatively damaged HK-2 cells. The results showed that after repair by UPPs, cell viability was enhanced, healing ability was improved, the intracellular superoxide dismutase level and mitochondrial membrane potential were increased, malondialdehyde, reactive oxygen species, and intracellular Ca<sup>2+</sup> levels were reduced, cellular autophagy was reduced; lysosomal integrity was improved, and cytoskeleton and cell morphology were restored. The ability of repaired cells to endocytose nano-calcium oxalate dihydrate crystals (nano−COD) was enhanced. The activity of UPPs was closely related to their –OSO<sub>3</sub><sup>−</sup> content. A too high or too low –OSO<sub>3</sub><sup>−</sup> content was not conducive to polysaccharide activity, and only UPP2 exhibited the best cell repair ability and strongest ability to promote the cell endocytosis of crystals. UPP2 may be used as a potential agent to inhibit CaOx crystal deposition caused by high oxalate concentration. |
| first_indexed | 2024-03-11T03:59:17Z |
| format | Article |
| id | doaj.art-1388c69caa114259986f3c0b3f759e5e |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T03:59:17Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-1388c69caa114259986f3c0b3f759e5e2023-11-18T00:14:13ZengMDPI AGAntioxidants2076-39212023-04-01125101510.3390/antiox12051015Sulfated <i>Undaria pinnatifida</i> Polysaccharide Promotes Endocytosis of Nano-Calcium Oxalate Dihydrate by Repairing Subcellular Organelles in HK-2 CellsXue-Wu Chen0Yu-Yun Zheng1Jian-Ming Ouyang2Institute of Biomineralization and Lithiasis Research, College of Chemistry and Materials Science, Jinan University, Guangzhou 510632, ChinaInstitute of Biomineralization and Lithiasis Research, College of Chemistry and Materials Science, Jinan University, Guangzhou 510632, ChinaInstitute of Biomineralization and Lithiasis Research, College of Chemistry and Materials Science, Jinan University, Guangzhou 510632, ChinaThe clinical manifestation of primary hyperoxaluria includes hyperoxaluria and recurrent urinary calculi. In this study, an oxidative damage model was constructed based on oxalate damage to the human renal proximal tubular epithelial cells (HK-2), and a comparative study was carried out on four different sulfated levels of <i>Undaria pinnatifida</i> polysaccharides (UPP0, UPP1, UPP2, and UPP3 with sulfate group [–OSO<sub>3</sub><sup>−</sup>] contents of 1.59%, 6.03%, 20.83%, and 36.39%, respectively) on the repair of oxidatively damaged HK-2 cells. The results showed that after repair by UPPs, cell viability was enhanced, healing ability was improved, the intracellular superoxide dismutase level and mitochondrial membrane potential were increased, malondialdehyde, reactive oxygen species, and intracellular Ca<sup>2+</sup> levels were reduced, cellular autophagy was reduced; lysosomal integrity was improved, and cytoskeleton and cell morphology were restored. The ability of repaired cells to endocytose nano-calcium oxalate dihydrate crystals (nano−COD) was enhanced. The activity of UPPs was closely related to their –OSO<sub>3</sub><sup>−</sup> content. A too high or too low –OSO<sub>3</sub><sup>−</sup> content was not conducive to polysaccharide activity, and only UPP2 exhibited the best cell repair ability and strongest ability to promote the cell endocytosis of crystals. UPP2 may be used as a potential agent to inhibit CaOx crystal deposition caused by high oxalate concentration.https://www.mdpi.com/2076-3921/12/5/1015<i>Undaria pinnatifida</i> polysaccharideacidic groupscell repaircrystal endocytosisprimary hyperoxaluria |
| spellingShingle | Xue-Wu Chen Yu-Yun Zheng Jian-Ming Ouyang Sulfated <i>Undaria pinnatifida</i> Polysaccharide Promotes Endocytosis of Nano-Calcium Oxalate Dihydrate by Repairing Subcellular Organelles in HK-2 Cells Antioxidants <i>Undaria pinnatifida</i> polysaccharide acidic groups cell repair crystal endocytosis primary hyperoxaluria |
| title | Sulfated <i>Undaria pinnatifida</i> Polysaccharide Promotes Endocytosis of Nano-Calcium Oxalate Dihydrate by Repairing Subcellular Organelles in HK-2 Cells |
| title_full | Sulfated <i>Undaria pinnatifida</i> Polysaccharide Promotes Endocytosis of Nano-Calcium Oxalate Dihydrate by Repairing Subcellular Organelles in HK-2 Cells |
| title_fullStr | Sulfated <i>Undaria pinnatifida</i> Polysaccharide Promotes Endocytosis of Nano-Calcium Oxalate Dihydrate by Repairing Subcellular Organelles in HK-2 Cells |
| title_full_unstemmed | Sulfated <i>Undaria pinnatifida</i> Polysaccharide Promotes Endocytosis of Nano-Calcium Oxalate Dihydrate by Repairing Subcellular Organelles in HK-2 Cells |
| title_short | Sulfated <i>Undaria pinnatifida</i> Polysaccharide Promotes Endocytosis of Nano-Calcium Oxalate Dihydrate by Repairing Subcellular Organelles in HK-2 Cells |
| title_sort | sulfated i undaria pinnatifida i polysaccharide promotes endocytosis of nano calcium oxalate dihydrate by repairing subcellular organelles in hk 2 cells |
| topic | <i>Undaria pinnatifida</i> polysaccharide acidic groups cell repair crystal endocytosis primary hyperoxaluria |
| url | https://www.mdpi.com/2076-3921/12/5/1015 |
| work_keys_str_mv | AT xuewuchen sulfatediundariapinnatifidaipolysaccharidepromotesendocytosisofnanocalciumoxalatedihydratebyrepairingsubcellularorganellesinhk2cells AT yuyunzheng sulfatediundariapinnatifidaipolysaccharidepromotesendocytosisofnanocalciumoxalatedihydratebyrepairingsubcellularorganellesinhk2cells AT jianmingouyang sulfatediundariapinnatifidaipolysaccharidepromotesendocytosisofnanocalciumoxalatedihydratebyrepairingsubcellularorganellesinhk2cells |