SHP-2 promotes the maturation of oligodendrocyte precursor cells through Akt and ERK1/2 signaling in vitro.
BACKGROUND: Oligodendrocyte precursor cells (OPCs) differentiate into oligodendrocytes (OLs), which are responsible for myelination. Myelin is essential for saltatory nerve conduction in the vertebrate nervous system. However, the molecular mechanisms of maturation and myelination by oligodendrocyte...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3118803?pdf=render |
| _version_ | 1828175469931921408 |
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| author | Xiujie Liu Yuanyuan Li Yong Zhang Yan Lu Wei Guo Peng Liu Jiazhen Zhou Zhenghua Xiang Cheng He |
| author_facet | Xiujie Liu Yuanyuan Li Yong Zhang Yan Lu Wei Guo Peng Liu Jiazhen Zhou Zhenghua Xiang Cheng He |
| author_sort | Xiujie Liu |
| collection | DOAJ |
| description | BACKGROUND: Oligodendrocyte precursor cells (OPCs) differentiate into oligodendrocytes (OLs), which are responsible for myelination. Myelin is essential for saltatory nerve conduction in the vertebrate nervous system. However, the molecular mechanisms of maturation and myelination by oligodendrocytes remain elusive. METHODS AND FINDINGS: In the present study, we showed that maturation of oligodendrocytes was attenuated by sodium orthovanadate (a comprehensive inhibitor of tyrosine phosphatases) and PTPi IV (a specific inhibitor of SHP-2). It is also found that SHP-2 was persistently expressed during maturation process of OPCs. Down-regulation of endogenous SHP-2 led to impairment of oligodendrocytes maturation and this effect was triiodo-L-thyronine (T3) dependent. Furthermore, over-expression of SHP-2 was shown to promote maturation of oligodendrocytes. Finally, it has been identified that SHP-2 was involved in activation of Akt and extracellular-regulated kinases 1 and 2 (ERK1/2) induced by T3 in oligodendrocytes. CONCLUSIONS: SHP-2 promotes oligodendrocytes maturation via Akt and ERK1/2 signaling in vitro. |
| first_indexed | 2024-04-12T04:28:33Z |
| format | Article |
| id | doaj.art-138adde811664844b5dd3be98e74b171 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T04:28:33Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-138adde811664844b5dd3be98e74b1712022-12-22T03:47:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0166e2105810.1371/journal.pone.0021058SHP-2 promotes the maturation of oligodendrocyte precursor cells through Akt and ERK1/2 signaling in vitro.Xiujie LiuYuanyuan LiYong ZhangYan LuWei GuoPeng LiuJiazhen ZhouZhenghua XiangCheng HeBACKGROUND: Oligodendrocyte precursor cells (OPCs) differentiate into oligodendrocytes (OLs), which are responsible for myelination. Myelin is essential for saltatory nerve conduction in the vertebrate nervous system. However, the molecular mechanisms of maturation and myelination by oligodendrocytes remain elusive. METHODS AND FINDINGS: In the present study, we showed that maturation of oligodendrocytes was attenuated by sodium orthovanadate (a comprehensive inhibitor of tyrosine phosphatases) and PTPi IV (a specific inhibitor of SHP-2). It is also found that SHP-2 was persistently expressed during maturation process of OPCs. Down-regulation of endogenous SHP-2 led to impairment of oligodendrocytes maturation and this effect was triiodo-L-thyronine (T3) dependent. Furthermore, over-expression of SHP-2 was shown to promote maturation of oligodendrocytes. Finally, it has been identified that SHP-2 was involved in activation of Akt and extracellular-regulated kinases 1 and 2 (ERK1/2) induced by T3 in oligodendrocytes. CONCLUSIONS: SHP-2 promotes oligodendrocytes maturation via Akt and ERK1/2 signaling in vitro.http://europepmc.org/articles/PMC3118803?pdf=render |
| spellingShingle | Xiujie Liu Yuanyuan Li Yong Zhang Yan Lu Wei Guo Peng Liu Jiazhen Zhou Zhenghua Xiang Cheng He SHP-2 promotes the maturation of oligodendrocyte precursor cells through Akt and ERK1/2 signaling in vitro. PLoS ONE |
| title | SHP-2 promotes the maturation of oligodendrocyte precursor cells through Akt and ERK1/2 signaling in vitro. |
| title_full | SHP-2 promotes the maturation of oligodendrocyte precursor cells through Akt and ERK1/2 signaling in vitro. |
| title_fullStr | SHP-2 promotes the maturation of oligodendrocyte precursor cells through Akt and ERK1/2 signaling in vitro. |
| title_full_unstemmed | SHP-2 promotes the maturation of oligodendrocyte precursor cells through Akt and ERK1/2 signaling in vitro. |
| title_short | SHP-2 promotes the maturation of oligodendrocyte precursor cells through Akt and ERK1/2 signaling in vitro. |
| title_sort | shp 2 promotes the maturation of oligodendrocyte precursor cells through akt and erk1 2 signaling in vitro |
| url | http://europepmc.org/articles/PMC3118803?pdf=render |
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