Increased apoptosis, but not pancreatic duodenal homeobox-1 expression in pancreatic islets is associated with intermittent glucose loads in mice
Background: Several caloric restriction studies revealed good for diabetes prevention. However, prevalence of it seems rising yearly. It needs alternative technique thus people can choose suitable way for them. Aim: To determine the effect of glucose diet intermittently on pancreatic duodenal homeob...
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| Format: | Article |
| Language: | English |
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Endocrinology Research Centre
2018-12-01
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| Series: | Сахарный диабет |
| Subjects: | |
| Online Access: | https://www.dia-endojournals.ru/jour/article/view/9437 |
| _version_ | 1797255120975560704 |
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| author | Lilik Herawati Kristanti Wanito Wigati Purwo Sri Rejeki Widjiati Widjiati Roedi Irawan |
| author_facet | Lilik Herawati Kristanti Wanito Wigati Purwo Sri Rejeki Widjiati Widjiati Roedi Irawan |
| author_sort | Lilik Herawati |
| collection | DOAJ |
| description | Background: Several caloric restriction studies revealed good for diabetes prevention. However, prevalence of it seems rising yearly. It needs alternative technique thus people can choose suitable way for them. Aim: To determine the effect of glucose diet intermittently on pancreatic duodenal homeobox-1 (PDX-1), apoptosis in pancreatic islets, and pancreatic islets area. Materials and methods: Balb/c mice were divided into five groups. Control group was given standard diet. The Continuous group was given standard diet and added with 7.4% calories continuously. The 1x, 2x, and 3x intermittent groups were given standard diet and added 7.4% calories for 1x, 2x, and 3x/week respectively. The 7.4% calorie addition was a glucose solution by oral galvage and ad libitum for 8 weeks. Results: There was a significantly difference on apoptosis density (p=0.043), but not in PDX-1. The islets Int2x and Int3x groups showed a significant decrease than control group (p=0.048). Insulin serum levels increased significantly in Continuous group compared to control group (p=0.04). In addition, the insulin serum level of 1x and 3x intermittent groups were significantly lower than Continuous group (p<0.05). Pre-post blood glucose levels on treatment groups decreased significantly compared to control group (p=0.012). Conclusions: Continuous and 1-3x/week intermittent addition of 7.4% calories of glucose for 8 weeks indicate a compensation mechanism for maintaining homeostasis, such as increase insulin serum level and seem to initiate the changes of morphologic-biomolecular (mainly apoptosis density in islets). The better mode is 1x/week of additional calories. However it needs further exploration to find out other influenced factors for these mechanism discovery. |
| first_indexed | 2024-03-08T15:19:53Z |
| format | Article |
| id | doaj.art-1393bd0d66de49aebd4325ec16de3a22 |
| institution | Directory Open Access Journal |
| issn | 2072-0351 2072-0378 |
| language | English |
| last_indexed | 2024-04-24T22:00:48Z |
| publishDate | 2018-12-01 |
| publisher | Endocrinology Research Centre |
| record_format | Article |
| series | Сахарный диабет |
| spelling | doaj.art-1393bd0d66de49aebd4325ec16de3a222024-03-20T11:48:02ZengEndocrinology Research CentreСахарный диабет2072-03512072-03782018-12-0121649750510.14341/DM94378584Increased apoptosis, but not pancreatic duodenal homeobox-1 expression in pancreatic islets is associated with intermittent glucose loads in miceLilik Herawati0Kristanti Wanito Wigati1Purwo Sri Rejeki2Widjiati Widjiati3Roedi Irawan4<p>Department of Physiology, Faculty of Medicine, Universitas Airlangga</p><p>Department of Physiology, Faculty of Medicine, Universitas Airlangga</p><p>Department of Physiology, Faculty of Medicine, Universitas Airlangga</p><p>Department of Veterinary Anatomy, Faculty of Veterinary, Universitas Airlangga</p><p>Department of Pediatric, Faculty of Medicine, Universitas Airlangga</p>Background: Several caloric restriction studies revealed good for diabetes prevention. However, prevalence of it seems rising yearly. It needs alternative technique thus people can choose suitable way for them. Aim: To determine the effect of glucose diet intermittently on pancreatic duodenal homeobox-1 (PDX-1), apoptosis in pancreatic islets, and pancreatic islets area. Materials and methods: Balb/c mice were divided into five groups. Control group was given standard diet. The Continuous group was given standard diet and added with 7.4% calories continuously. The 1x, 2x, and 3x intermittent groups were given standard diet and added 7.4% calories for 1x, 2x, and 3x/week respectively. The 7.4% calorie addition was a glucose solution by oral galvage and ad libitum for 8 weeks. Results: There was a significantly difference on apoptosis density (p=0.043), but not in PDX-1. The islets Int2x and Int3x groups showed a significant decrease than control group (p=0.048). Insulin serum levels increased significantly in Continuous group compared to control group (p=0.04). In addition, the insulin serum level of 1x and 3x intermittent groups were significantly lower than Continuous group (p<0.05). Pre-post blood glucose levels on treatment groups decreased significantly compared to control group (p=0.012). Conclusions: Continuous and 1-3x/week intermittent addition of 7.4% calories of glucose for 8 weeks indicate a compensation mechanism for maintaining homeostasis, such as increase insulin serum level and seem to initiate the changes of morphologic-biomolecular (mainly apoptosis density in islets). The better mode is 1x/week of additional calories. However it needs further exploration to find out other influenced factors for these mechanism discovery.https://www.dia-endojournals.ru/jour/article/view/9437pdx-1apoptosispancreatic areaglucose, insulin |
| spellingShingle | Lilik Herawati Kristanti Wanito Wigati Purwo Sri Rejeki Widjiati Widjiati Roedi Irawan Increased apoptosis, but not pancreatic duodenal homeobox-1 expression in pancreatic islets is associated with intermittent glucose loads in mice Сахарный диабет pdx-1 apoptosis pancreatic area glucose, insulin |
| title | Increased apoptosis, but not pancreatic duodenal homeobox-1 expression in pancreatic islets is associated with intermittent glucose loads in mice |
| title_full | Increased apoptosis, but not pancreatic duodenal homeobox-1 expression in pancreatic islets is associated with intermittent glucose loads in mice |
| title_fullStr | Increased apoptosis, but not pancreatic duodenal homeobox-1 expression in pancreatic islets is associated with intermittent glucose loads in mice |
| title_full_unstemmed | Increased apoptosis, but not pancreatic duodenal homeobox-1 expression in pancreatic islets is associated with intermittent glucose loads in mice |
| title_short | Increased apoptosis, but not pancreatic duodenal homeobox-1 expression in pancreatic islets is associated with intermittent glucose loads in mice |
| title_sort | increased apoptosis but not pancreatic duodenal homeobox 1 expression in pancreatic islets is associated with intermittent glucose loads in mice |
| topic | pdx-1 apoptosis pancreatic area glucose, insulin |
| url | https://www.dia-endojournals.ru/jour/article/view/9437 |
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