SAM Composition and Electrode Roughness Affect Performance of a DNA Biosensor for Antibiotic Resistance
Antibiotic resistance is a growing concern in the treatment of infectious disease worldwide. Point-of-care (PoC) assays which rapidly identify antibiotic resistance in a sample will allow for immediate targeted therapy which improves patient outcomes and helps maintain the effectiveness of current a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-02-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/9/1/22 |
| _version_ | 1818314137032196096 |
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| author | Adrian Butterworth Elizabeth Blues Paul Williamson Milovan Cardona Louise Gray Damion K Corrigan |
| author_facet | Adrian Butterworth Elizabeth Blues Paul Williamson Milovan Cardona Louise Gray Damion K Corrigan |
| author_sort | Adrian Butterworth |
| collection | DOAJ |
| description | Antibiotic resistance is a growing concern in the treatment of infectious disease worldwide. Point-of-care (PoC) assays which rapidly identify antibiotic resistance in a sample will allow for immediate targeted therapy which improves patient outcomes and helps maintain the effectiveness of current antibiotic stockpiles. Electrochemical assays offer many benefits, but translation from a benchtop measurement system to low-cost portable electrodes can be challenging. Using electrochemical and physical techniques, this study examines how different electrode surfaces and bio-recognition elements, i.e. the self-assembled monolayer (SAM), affect the performance of a biosensor measuring the hybridisation of a probe for antibiotic resistance to a target gene sequence in solution. We evaluate several commercially available electrodes which could be suitable for PoC testing with different SAM layers and show that electrode selection also plays an important role in overall biosensor performance. |
| first_indexed | 2024-12-13T08:44:51Z |
| format | Article |
| id | doaj.art-1399cd6bcb974682940aa79464bac9c9 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-12-13T08:44:51Z |
| publishDate | 2019-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-1399cd6bcb974682940aa79464bac9c92022-12-21T23:53:28ZengMDPI AGBiosensors2079-63742019-02-01912210.3390/bios9010022bios9010022SAM Composition and Electrode Roughness Affect Performance of a DNA Biosensor for Antibiotic ResistanceAdrian Butterworth0Elizabeth Blues1Paul Williamson2Milovan Cardona3Louise Gray4Damion K Corrigan5Department of Biomedical Engineering, Wolfson Centre, 106 Rottenrow East, University of Strathclyde, Glasgow G1 1XQ, UKDepartment of Biomedical Engineering, Wolfson Centre, 106 Rottenrow East, University of Strathclyde, Glasgow G1 1XQ, UKDepartment of Biomedical Engineering, Wolfson Centre, 106 Rottenrow East, University of Strathclyde, Glasgow G1 1XQ, UKDepartment of Biomedical Engineering, Wolfson Centre, 106 Rottenrow East, University of Strathclyde, Glasgow G1 1XQ, UKFlexMedical Solutions, Eliburn Industrial Park, Livingston, EH54 6GQ, Scotland, UKDepartment of Biomedical Engineering, Wolfson Centre, 106 Rottenrow East, University of Strathclyde, Glasgow G1 1XQ, UKAntibiotic resistance is a growing concern in the treatment of infectious disease worldwide. Point-of-care (PoC) assays which rapidly identify antibiotic resistance in a sample will allow for immediate targeted therapy which improves patient outcomes and helps maintain the effectiveness of current antibiotic stockpiles. Electrochemical assays offer many benefits, but translation from a benchtop measurement system to low-cost portable electrodes can be challenging. Using electrochemical and physical techniques, this study examines how different electrode surfaces and bio-recognition elements, i.e. the self-assembled monolayer (SAM), affect the performance of a biosensor measuring the hybridisation of a probe for antibiotic resistance to a target gene sequence in solution. We evaluate several commercially available electrodes which could be suitable for PoC testing with different SAM layers and show that electrode selection also plays an important role in overall biosensor performance.https://www.mdpi.com/2079-6374/9/1/22electrochemical biosensorDNA detectionpoint-of-care diagnosticselectrochemical impedance spectroscopyself-assembled monolayers (SAMs) |
| spellingShingle | Adrian Butterworth Elizabeth Blues Paul Williamson Milovan Cardona Louise Gray Damion K Corrigan SAM Composition and Electrode Roughness Affect Performance of a DNA Biosensor for Antibiotic Resistance Biosensors electrochemical biosensor DNA detection point-of-care diagnostics electrochemical impedance spectroscopy self-assembled monolayers (SAMs) |
| title | SAM Composition and Electrode Roughness Affect Performance of a DNA Biosensor for Antibiotic Resistance |
| title_full | SAM Composition and Electrode Roughness Affect Performance of a DNA Biosensor for Antibiotic Resistance |
| title_fullStr | SAM Composition and Electrode Roughness Affect Performance of a DNA Biosensor for Antibiotic Resistance |
| title_full_unstemmed | SAM Composition and Electrode Roughness Affect Performance of a DNA Biosensor for Antibiotic Resistance |
| title_short | SAM Composition and Electrode Roughness Affect Performance of a DNA Biosensor for Antibiotic Resistance |
| title_sort | sam composition and electrode roughness affect performance of a dna biosensor for antibiotic resistance |
| topic | electrochemical biosensor DNA detection point-of-care diagnostics electrochemical impedance spectroscopy self-assembled monolayers (SAMs) |
| url | https://www.mdpi.com/2079-6374/9/1/22 |
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