In vivo characterization of the bacterial intramembrane-cleaving protease RseP using the heme binding tag-based assay iCliPSpy
iCLiPSpy is a simple assay to identify residues important for substrate binding and activity of intramembrane-cleaving proteases (I-CLiPs) in vivo and can be adapted to a screening assay for I-CLiP inhibitor or activator discovery.
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-03-01
|
| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-023-04654-z |
| _version_ | 1797863784938733568 |
|---|---|
| author | Thomas Kupke Rabea M. Götz Florian M. Richter Rainer Beck Fabio Lolicato Walter Nickel Carsten Hopf Britta Brügger |
| author_facet | Thomas Kupke Rabea M. Götz Florian M. Richter Rainer Beck Fabio Lolicato Walter Nickel Carsten Hopf Britta Brügger |
| author_sort | Thomas Kupke |
| collection | DOAJ |
| description | iCLiPSpy is a simple assay to identify residues important for substrate binding and activity of intramembrane-cleaving proteases (I-CLiPs) in vivo and can be adapted to a screening assay for I-CLiP inhibitor or activator discovery. |
| first_indexed | 2024-04-09T22:42:16Z |
| format | Article |
| id | doaj.art-139c8a25f23549788a6455f908f3539f |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2024-04-09T22:42:16Z |
| publishDate | 2023-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-139c8a25f23549788a6455f908f3539f2023-03-22T12:05:19ZengNature PortfolioCommunications Biology2399-36422023-03-016111510.1038/s42003-023-04654-zIn vivo characterization of the bacterial intramembrane-cleaving protease RseP using the heme binding tag-based assay iCliPSpyThomas Kupke0Rabea M. Götz1Florian M. Richter2Rainer Beck3Fabio Lolicato4Walter Nickel5Carsten Hopf6Britta Brügger7Heidelberg University Biochemistry Center (BZH), Im Neuenheimer Feld 328Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied SciencesCenter for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied SciencesHeidelberg University Biochemistry Center (BZH), Im Neuenheimer Feld 328Heidelberg University Biochemistry Center (BZH), Im Neuenheimer Feld 328Heidelberg University Biochemistry Center (BZH), Im Neuenheimer Feld 328Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied SciencesHeidelberg University Biochemistry Center (BZH), Im Neuenheimer Feld 328iCLiPSpy is a simple assay to identify residues important for substrate binding and activity of intramembrane-cleaving proteases (I-CLiPs) in vivo and can be adapted to a screening assay for I-CLiP inhibitor or activator discovery.https://doi.org/10.1038/s42003-023-04654-z |
| spellingShingle | Thomas Kupke Rabea M. Götz Florian M. Richter Rainer Beck Fabio Lolicato Walter Nickel Carsten Hopf Britta Brügger In vivo characterization of the bacterial intramembrane-cleaving protease RseP using the heme binding tag-based assay iCliPSpy Communications Biology |
| title | In vivo characterization of the bacterial intramembrane-cleaving protease RseP using the heme binding tag-based assay iCliPSpy |
| title_full | In vivo characterization of the bacterial intramembrane-cleaving protease RseP using the heme binding tag-based assay iCliPSpy |
| title_fullStr | In vivo characterization of the bacterial intramembrane-cleaving protease RseP using the heme binding tag-based assay iCliPSpy |
| title_full_unstemmed | In vivo characterization of the bacterial intramembrane-cleaving protease RseP using the heme binding tag-based assay iCliPSpy |
| title_short | In vivo characterization of the bacterial intramembrane-cleaving protease RseP using the heme binding tag-based assay iCliPSpy |
| title_sort | in vivo characterization of the bacterial intramembrane cleaving protease rsep using the heme binding tag based assay iclipspy |
| url | https://doi.org/10.1038/s42003-023-04654-z |
| work_keys_str_mv | AT thomaskupke invivocharacterizationofthebacterialintramembranecleavingproteasersepusingthehemebindingtagbasedassayiclipspy AT rabeamgotz invivocharacterizationofthebacterialintramembranecleavingproteasersepusingthehemebindingtagbasedassayiclipspy AT florianmrichter invivocharacterizationofthebacterialintramembranecleavingproteasersepusingthehemebindingtagbasedassayiclipspy AT rainerbeck invivocharacterizationofthebacterialintramembranecleavingproteasersepusingthehemebindingtagbasedassayiclipspy AT fabiololicato invivocharacterizationofthebacterialintramembranecleavingproteasersepusingthehemebindingtagbasedassayiclipspy AT walternickel invivocharacterizationofthebacterialintramembranecleavingproteasersepusingthehemebindingtagbasedassayiclipspy AT carstenhopf invivocharacterizationofthebacterialintramembranecleavingproteasersepusingthehemebindingtagbasedassayiclipspy AT brittabrugger invivocharacterizationofthebacterialintramembranecleavingproteasersepusingthehemebindingtagbasedassayiclipspy |