Acute and Sub-Acute Oral Toxicity Evaluation of Astragalus hamosus Seedpod Ethanolic Extract in Wistar Rats
Background: Oral consumption of Astragalus hamosus L. (AH) seedpod has been widely prescribed in traditional medicine system. However, its toxicity evaluation has never been investigated. Hence, the current study was performed to evaluate the toxicological profile of AH seedpod in acute and subacute...
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2018-03-01
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| Series: | Pharmaceutical Sciences |
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| Online Access: | http://journals.tbzmed.ac.ir/PHARM/Manuscript/PHARM-24-23.pdf |
| _version_ | 1818874941911597056 |
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| author | Mohammadmehdi Hassanzadeh-Taheri Mehran Hosseini Mojtaba Salimi Hesam Moodi Davood Dorranpour |
| author_facet | Mohammadmehdi Hassanzadeh-Taheri Mehran Hosseini Mojtaba Salimi Hesam Moodi Davood Dorranpour |
| author_sort | Mohammadmehdi Hassanzadeh-Taheri |
| collection | DOAJ |
| description | Background: Oral consumption of Astragalus hamosus L. (AH) seedpod has been widely prescribed in traditional medicine system. However, its toxicity evaluation has never been investigated. Hence, the current study was performed to evaluate the toxicological profile of AH seedpod in acute and subacute assessments based on the OECD-guidelines 425 and 407 in male and female Wistar rats. Methods: In the acute study, ethanolic extract of AH at a single dose of 2000 mg/kg was orally administrated to six female rats. In the subacute assay, AH at the three different oral doses (75, 150 and 300 mg/kg) were administrated to both male and female rats for 28 consecutive days. Results: No death or behavioural changes were observed in the treated animals. In subacute test, in both sexes, no changes in organ weights observed. Biochemically, compared to the control, AH at the dose of 300 mg/kg slightly increased (p<0.05) uric acid and creatinine and declined total cholesterol levels in both male and female rats. However, there is no statistically difference in other parameters such as albumin, triglyceride, blood urea, aspartate aminotransferase and alanine aminotransferase between AH treated groups and untreated controls. Hematologic parameters showed that AH at the maximum dose decreased red blood cells count only in male rats. Histopathological evaluation of liver and kidney exhibited no noticeable alterations in AH treated animals. Conclusion: It could be concluded that high excessive and long term consumption of AH may lead to renal dysfunction and deficiency in hematopoietic system. |
| first_indexed | 2024-12-19T13:18:36Z |
| format | Article |
| id | doaj.art-139e0341be9648ecb3fbacd1bcdc492b |
| institution | Directory Open Access Journal |
| issn | 1735-403X 2383-2886 |
| language | English |
| last_indexed | 2024-12-19T13:18:36Z |
| publishDate | 2018-03-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Pharmaceutical Sciences |
| spelling | doaj.art-139e0341be9648ecb3fbacd1bcdc492b2022-12-21T20:19:45ZengTabriz University of Medical SciencesPharmaceutical Sciences1735-403X2383-28862018-03-01241233010.15171/PS.2018.05PHARM_19668_20171014213733Acute and Sub-Acute Oral Toxicity Evaluation of Astragalus hamosus Seedpod Ethanolic Extract in Wistar RatsMohammadmehdi Hassanzadeh-Taheri0Mehran Hosseini1Mojtaba Salimi2Hesam Moodi3Davood Dorranpour4Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran.Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran.Department of Anatomy, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.Department of Anatomy, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.Department of Anatomy, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.Background: Oral consumption of Astragalus hamosus L. (AH) seedpod has been widely prescribed in traditional medicine system. However, its toxicity evaluation has never been investigated. Hence, the current study was performed to evaluate the toxicological profile of AH seedpod in acute and subacute assessments based on the OECD-guidelines 425 and 407 in male and female Wistar rats. Methods: In the acute study, ethanolic extract of AH at a single dose of 2000 mg/kg was orally administrated to six female rats. In the subacute assay, AH at the three different oral doses (75, 150 and 300 mg/kg) were administrated to both male and female rats for 28 consecutive days. Results: No death or behavioural changes were observed in the treated animals. In subacute test, in both sexes, no changes in organ weights observed. Biochemically, compared to the control, AH at the dose of 300 mg/kg slightly increased (p<0.05) uric acid and creatinine and declined total cholesterol levels in both male and female rats. However, there is no statistically difference in other parameters such as albumin, triglyceride, blood urea, aspartate aminotransferase and alanine aminotransferase between AH treated groups and untreated controls. Hematologic parameters showed that AH at the maximum dose decreased red blood cells count only in male rats. Histopathological evaluation of liver and kidney exhibited no noticeable alterations in AH treated animals. Conclusion: It could be concluded that high excessive and long term consumption of AH may lead to renal dysfunction and deficiency in hematopoietic system.http://journals.tbzmed.ac.ir/PHARM/Manuscript/PHARM-24-23.pdfAstragalus hamosusEthanolic extractAcute toxicity studySubacute toxicity studyRat |
| spellingShingle | Mohammadmehdi Hassanzadeh-Taheri Mehran Hosseini Mojtaba Salimi Hesam Moodi Davood Dorranpour Acute and Sub-Acute Oral Toxicity Evaluation of Astragalus hamosus Seedpod Ethanolic Extract in Wistar Rats Pharmaceutical Sciences Astragalus hamosus Ethanolic extract Acute toxicity study Subacute toxicity study Rat |
| title | Acute and Sub-Acute Oral Toxicity Evaluation of Astragalus hamosus Seedpod Ethanolic Extract in Wistar Rats |
| title_full | Acute and Sub-Acute Oral Toxicity Evaluation of Astragalus hamosus Seedpod Ethanolic Extract in Wistar Rats |
| title_fullStr | Acute and Sub-Acute Oral Toxicity Evaluation of Astragalus hamosus Seedpod Ethanolic Extract in Wistar Rats |
| title_full_unstemmed | Acute and Sub-Acute Oral Toxicity Evaluation of Astragalus hamosus Seedpod Ethanolic Extract in Wistar Rats |
| title_short | Acute and Sub-Acute Oral Toxicity Evaluation of Astragalus hamosus Seedpod Ethanolic Extract in Wistar Rats |
| title_sort | acute and sub acute oral toxicity evaluation of astragalus hamosus seedpod ethanolic extract in wistar rats |
| topic | Astragalus hamosus Ethanolic extract Acute toxicity study Subacute toxicity study Rat |
| url | http://journals.tbzmed.ac.ir/PHARM/Manuscript/PHARM-24-23.pdf |
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