Assessment of Antibacterial and Anti-biofilm Effects of Vitamin C Against Pseudomonas aeruginosa Clinical Isolates
There is a persistent need to look for alternative therapeutic modalities to help control the pandemic of antimicrobial resistance. Assessment of antibacterial and anti-biofilm effects of vitamin C (ascorbic acid) was the aim of the current study. The micro-dilution method determined the minimal inh...
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.847449/full |
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| author | Wedad M. Abdelraheem Marwa M. M. Refaie Rehab Kamal Mohamed Yousef Aliaa S. Abd El Fatah Yosra M. Mousa Rabab Rashwan |
| author_facet | Wedad M. Abdelraheem Marwa M. M. Refaie Rehab Kamal Mohamed Yousef Aliaa S. Abd El Fatah Yosra M. Mousa Rabab Rashwan |
| author_sort | Wedad M. Abdelraheem |
| collection | DOAJ |
| description | There is a persistent need to look for alternative therapeutic modalities to help control the pandemic of antimicrobial resistance. Assessment of antibacterial and anti-biofilm effects of vitamin C (ascorbic acid) was the aim of the current study. The micro-dilution method determined the minimal inhibitory concentration (MIC) of ascorbic acid or antibiotics alone and in combinations against Pseudomonas aeruginosa (P. aeruginosa) clinical isolates. The micro-titer plate method monitored the effect of ascorbic acid on the biofilm-producing isolates of P. aeruginosa. The effect of ascorbic acid on the differential expression of different antibiotic-resistant genes and biofilm encoding genes of P. aeruginosa isolates were also tested using real-time polymerase chain reaction (PCR). For in vivo assessment of the antibacterial effects of ascorbic acid alone or combined with an antibiotic, rats were infected with P. aeruginosa clinical isolate followed by different treatment regimens. MICs of ascorbic acid among P. aeruginosa isolates were in the range of 156.2–1,250 μg/ml, while MIC50 and MIC90 were 312.5 and 625 μg/ml, respectively. At sub-inhibitory concentrations (19.5–312.5 μg/ml), ascorbic acid had 100% biofilm inhibitory effect. Furthermore, ascorbic acid-treated bacteria showed downregulation of genes underpinning biofilm formation and antibiotic resistance. In vivo assessment of vitamin C and ceftazidime in rats showed that administration of both at a lower dose for treatment of pseudomonas infection in rats had a synergistic and more powerful effect. Vitamin C shows excellent in vitro results as an antibacterial and anti-biofilm agent. Vitamin C should be routinely prescribed with antibiotics to treat bacterial infections in the clinical setting. |
| first_indexed | 2024-04-12T16:13:22Z |
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| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T16:13:22Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-13a187fdfb0840dab02e93e9998a57352022-12-22T03:25:50ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-05-011310.3389/fmicb.2022.847449847449Assessment of Antibacterial and Anti-biofilm Effects of Vitamin C Against Pseudomonas aeruginosa Clinical IsolatesWedad M. Abdelraheem0Marwa M. M. Refaie1Rehab Kamal Mohamed Yousef2Aliaa S. Abd El Fatah3Yosra M. Mousa4Rabab Rashwan5Department of Medical Microbiology and Immunology, Faculty of Medicine, Minia University, Minya, EgyptDepartment of Pharmacology, Faculty of Medicine, Minia University, Minya, EgyptDepartment of Pathology, Faculty of Medicine, Minia University, Minya, EgyptInternal Medicine Department, Faculty of Medicine, Minia University, Minya, EgyptChest Department, Faculty of Medicine, Minia University, Minya, EgyptDepartment of Medical Microbiology and Immunology, Faculty of Medicine, Minia University, Minya, EgyptThere is a persistent need to look for alternative therapeutic modalities to help control the pandemic of antimicrobial resistance. Assessment of antibacterial and anti-biofilm effects of vitamin C (ascorbic acid) was the aim of the current study. The micro-dilution method determined the minimal inhibitory concentration (MIC) of ascorbic acid or antibiotics alone and in combinations against Pseudomonas aeruginosa (P. aeruginosa) clinical isolates. The micro-titer plate method monitored the effect of ascorbic acid on the biofilm-producing isolates of P. aeruginosa. The effect of ascorbic acid on the differential expression of different antibiotic-resistant genes and biofilm encoding genes of P. aeruginosa isolates were also tested using real-time polymerase chain reaction (PCR). For in vivo assessment of the antibacterial effects of ascorbic acid alone or combined with an antibiotic, rats were infected with P. aeruginosa clinical isolate followed by different treatment regimens. MICs of ascorbic acid among P. aeruginosa isolates were in the range of 156.2–1,250 μg/ml, while MIC50 and MIC90 were 312.5 and 625 μg/ml, respectively. At sub-inhibitory concentrations (19.5–312.5 μg/ml), ascorbic acid had 100% biofilm inhibitory effect. Furthermore, ascorbic acid-treated bacteria showed downregulation of genes underpinning biofilm formation and antibiotic resistance. In vivo assessment of vitamin C and ceftazidime in rats showed that administration of both at a lower dose for treatment of pseudomonas infection in rats had a synergistic and more powerful effect. Vitamin C shows excellent in vitro results as an antibacterial and anti-biofilm agent. Vitamin C should be routinely prescribed with antibiotics to treat bacterial infections in the clinical setting.https://www.frontiersin.org/articles/10.3389/fmicb.2022.847449/fullantimicrobial and anti-biofilm effects of vitamin Canti-biofilm strategiessynergismMDRbiofilm former P. aeruginosa strains |
| spellingShingle | Wedad M. Abdelraheem Marwa M. M. Refaie Rehab Kamal Mohamed Yousef Aliaa S. Abd El Fatah Yosra M. Mousa Rabab Rashwan Assessment of Antibacterial and Anti-biofilm Effects of Vitamin C Against Pseudomonas aeruginosa Clinical Isolates Frontiers in Microbiology antimicrobial and anti-biofilm effects of vitamin C anti-biofilm strategies synergism MDR biofilm former P. aeruginosa strains |
| title | Assessment of Antibacterial and Anti-biofilm Effects of Vitamin C Against Pseudomonas aeruginosa Clinical Isolates |
| title_full | Assessment of Antibacterial and Anti-biofilm Effects of Vitamin C Against Pseudomonas aeruginosa Clinical Isolates |
| title_fullStr | Assessment of Antibacterial and Anti-biofilm Effects of Vitamin C Against Pseudomonas aeruginosa Clinical Isolates |
| title_full_unstemmed | Assessment of Antibacterial and Anti-biofilm Effects of Vitamin C Against Pseudomonas aeruginosa Clinical Isolates |
| title_short | Assessment of Antibacterial and Anti-biofilm Effects of Vitamin C Against Pseudomonas aeruginosa Clinical Isolates |
| title_sort | assessment of antibacterial and anti biofilm effects of vitamin c against pseudomonas aeruginosa clinical isolates |
| topic | antimicrobial and anti-biofilm effects of vitamin C anti-biofilm strategies synergism MDR biofilm former P. aeruginosa strains |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.847449/full |
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