The effect of water source and soil supplementation on parasite contamination in organic vegetable gardens
Abstract The objective of this study was to determine factors associated with vegetable contamination with zoonotic protozoan. Samples of water, soil and vegetables were collected from July/2014 to May/2016, totaling 83 samples, 21 properties of Londrina region, Paraná, Brazil. DNA amplification of...
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| Format: | Article |
| Language: | English |
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Colégio Brasileiro de Parasitologia Veterinaria
2018-08-01
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| Series: | Revista Brasileira de Parasitologia Veterinária |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018000300327&lng=en&tlng=en |
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| author | Fernanda Pinto Ferreira Eloiza Teles Caldart Roberta Lemos Freire Regina Mitsuka-Breganó Felipe Machado de Freitas Ana Carolina Miura Marcelle Mareze Felippe Danyel Cardoso Martins Mariana Ragassi Urbano Adilson Luiz Seifert Italmar Teodorico Navarro |
| author_facet | Fernanda Pinto Ferreira Eloiza Teles Caldart Roberta Lemos Freire Regina Mitsuka-Breganó Felipe Machado de Freitas Ana Carolina Miura Marcelle Mareze Felippe Danyel Cardoso Martins Mariana Ragassi Urbano Adilson Luiz Seifert Italmar Teodorico Navarro |
| author_sort | Fernanda Pinto Ferreira |
| collection | DOAJ |
| description | Abstract The objective of this study was to determine factors associated with vegetable contamination with zoonotic protozoan. Samples of water, soil and vegetables were collected from July/2014 to May/2016, totaling 83 samples, 21 properties of Londrina region, Paraná, Brazil. DNA amplification of Toxoplasma gondii, Cryptosporidium spp. and Giardia intestinalis in the samples was conducted using polymerase chain reaction (PCR). The PCR results were positive for T. gondii in 12.9% (8/62), Cryptosporidium spp. in 11.3% (7/62) and G. intestinalis in 25.8% (16/62) of the samples. DNA sequencing identified C. parvum in five samples and G. intestinalis Assemblage E in three. The statistical associations demonstrated greater probability of positive samples for T. gondii and for at least one of the three protozoa when the source of irrigation water was the river; a greater chance of positive samples for Cryptosporidium spp. when deer were present on the property; and a smaller chance of positive samples for at least one of the three etiologic agents when soil was supplemented with limestone. The results expose some critical contamination points, providing support for training farmers on good management practices during the production process. |
| first_indexed | 2024-12-12T05:14:38Z |
| format | Article |
| id | doaj.art-13a1abbed33e4976a5ee0234bc1b85ba |
| institution | Directory Open Access Journal |
| issn | 1984-2961 |
| language | English |
| last_indexed | 2024-12-12T05:14:38Z |
| publishDate | 2018-08-01 |
| publisher | Colégio Brasileiro de Parasitologia Veterinaria |
| record_format | Article |
| series | Revista Brasileira de Parasitologia Veterinária |
| spelling | doaj.art-13a1abbed33e4976a5ee0234bc1b85ba2022-12-22T00:36:49ZengColégio Brasileiro de Parasitologia VeterinariaRevista Brasileira de Parasitologia Veterinária1984-29612018-08-0127332733710.1590/s1984-296120180050S1984-29612018000300327The effect of water source and soil supplementation on parasite contamination in organic vegetable gardensFernanda Pinto FerreiraEloiza Teles CaldartRoberta Lemos FreireRegina Mitsuka-BreganóFelipe Machado de FreitasAna Carolina MiuraMarcelle MarezeFelippe Danyel Cardoso MartinsMariana Ragassi UrbanoAdilson Luiz SeifertItalmar Teodorico NavarroAbstract The objective of this study was to determine factors associated with vegetable contamination with zoonotic protozoan. Samples of water, soil and vegetables were collected from July/2014 to May/2016, totaling 83 samples, 21 properties of Londrina region, Paraná, Brazil. DNA amplification of Toxoplasma gondii, Cryptosporidium spp. and Giardia intestinalis in the samples was conducted using polymerase chain reaction (PCR). The PCR results were positive for T. gondii in 12.9% (8/62), Cryptosporidium spp. in 11.3% (7/62) and G. intestinalis in 25.8% (16/62) of the samples. DNA sequencing identified C. parvum in five samples and G. intestinalis Assemblage E in three. The statistical associations demonstrated greater probability of positive samples for T. gondii and for at least one of the three protozoa when the source of irrigation water was the river; a greater chance of positive samples for Cryptosporidium spp. when deer were present on the property; and a smaller chance of positive samples for at least one of the three etiologic agents when soil was supplemented with limestone. The results expose some critical contamination points, providing support for training farmers on good management practices during the production process.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018000300327&lng=en&tlng=enLegumeságuacontaminação ambientalCryptosporidium spp.Toxoplasma gondiiGiardia intestinalis |
| spellingShingle | Fernanda Pinto Ferreira Eloiza Teles Caldart Roberta Lemos Freire Regina Mitsuka-Breganó Felipe Machado de Freitas Ana Carolina Miura Marcelle Mareze Felippe Danyel Cardoso Martins Mariana Ragassi Urbano Adilson Luiz Seifert Italmar Teodorico Navarro The effect of water source and soil supplementation on parasite contamination in organic vegetable gardens Revista Brasileira de Parasitologia Veterinária Legumes água contaminação ambiental Cryptosporidium spp. Toxoplasma gondii Giardia intestinalis |
| title | The effect of water source and soil supplementation on parasite contamination in organic vegetable gardens |
| title_full | The effect of water source and soil supplementation on parasite contamination in organic vegetable gardens |
| title_fullStr | The effect of water source and soil supplementation on parasite contamination in organic vegetable gardens |
| title_full_unstemmed | The effect of water source and soil supplementation on parasite contamination in organic vegetable gardens |
| title_short | The effect of water source and soil supplementation on parasite contamination in organic vegetable gardens |
| title_sort | effect of water source and soil supplementation on parasite contamination in organic vegetable gardens |
| topic | Legumes água contaminação ambiental Cryptosporidium spp. Toxoplasma gondii Giardia intestinalis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018000300327&lng=en&tlng=en |
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