A versatile system to record cell-cell interactions
Cell-cell interactions influence all aspects of development, homeostasis, and disease. In cancer, interactions between cancer cells and stromal cells play a major role in nearly every step of carcinogenesis. Thus, the ability to record cell-cell interactions would facilitate mechanistic delineation...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2020-10-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/61080 |
| _version_ | 1811201445496619008 |
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| author | Rui Tang Christopher W Murray Ian L Linde Nicholas J Kramer Zhonglin Lyu Min K Tsai Leo C Chen Hongchen Cai Aaron D Gitler Edgar Engleman Wonjae Lee Monte M Winslow |
| author_facet | Rui Tang Christopher W Murray Ian L Linde Nicholas J Kramer Zhonglin Lyu Min K Tsai Leo C Chen Hongchen Cai Aaron D Gitler Edgar Engleman Wonjae Lee Monte M Winslow |
| author_sort | Rui Tang |
| collection | DOAJ |
| description | Cell-cell interactions influence all aspects of development, homeostasis, and disease. In cancer, interactions between cancer cells and stromal cells play a major role in nearly every step of carcinogenesis. Thus, the ability to record cell-cell interactions would facilitate mechanistic delineation of the role of the cancer microenvironment. Here, we describe GFP-based Touching Nexus (G-baToN) which relies upon nanobody-directed fluorescent protein transfer to enable sensitive and specific labeling of cells after cell-cell interactions. G-baToN is a generalizable system that enables physical contact-based labeling between various human and mouse cell types, including endothelial cell-pericyte, neuron-astrocyte, and diverse cancer-stromal cell pairs. A suite of orthogonal baToN tools enables reciprocal cell-cell labeling, interaction-dependent cargo transfer, and the identification of higher order cell-cell interactions across a wide range of cell types. The ability to track physically interacting cells with these simple and sensitive systems will greatly accelerate our understanding of the outputs of cell-cell interactions in cancer as well as across many biological processes. |
| first_indexed | 2024-04-12T02:20:46Z |
| format | Article |
| id | doaj.art-13a418d59ffc4122921111ddb10c3638 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:20:46Z |
| publishDate | 2020-10-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-13a418d59ffc4122921111ddb10c36382022-12-22T03:52:07ZengeLife Sciences Publications LtdeLife2050-084X2020-10-01910.7554/eLife.61080A versatile system to record cell-cell interactionsRui Tang0https://orcid.org/0000-0002-6950-9580Christopher W Murray1https://orcid.org/0000-0002-6551-2053Ian L Linde2Nicholas J Kramer3https://orcid.org/0000-0003-4557-8343Zhonglin Lyu4Min K Tsai5Leo C Chen6https://orcid.org/0000-0002-4950-0757Hongchen Cai7Aaron D Gitler8https://orcid.org/0000-0001-8603-1526Edgar Engleman9https://orcid.org/0000-0002-2096-9279Wonjae Lee10Monte M Winslow11https://orcid.org/0000-0002-5730-9573Department of Genetics, Stanford University School of Medicine, Stanford, United StatesCancer Biology Program, Stanford University School of Medicine, Stanford, United StatesImmunology Program, Stanford University School of Medicine, Stanford, United StatesDepartment of Genetics, Stanford University School of Medicine, Stanford, United States; Neuroscience Program, Stanford University School of Medicine, Stanford, United StatesDepartment of Neurosurgery, Stanford University School of Medicine, Stanford, United StatesDepartment of Genetics, Stanford University School of Medicine, Stanford, United StatesDepartment of Genetics, Stanford University School of Medicine, Stanford, United StatesDepartment of Genetics, Stanford University School of Medicine, Stanford, United StatesDepartment of Genetics, Stanford University School of Medicine, Stanford, United StatesCancer Biology Program, Stanford University School of Medicine, Stanford, United States; Immunology Program, Stanford University School of Medicine, Stanford, United States; Department of Pathology, Stanford University School of Medicine, Stanford, United StatesDepartment of Neurosurgery, Stanford University School of Medicine, Stanford, United StatesDepartment of Genetics, Stanford University School of Medicine, Stanford, United States; Cancer Biology Program, Stanford University School of Medicine, Stanford, United States; Department of Pathology, Stanford University School of Medicine, Stanford, United StatesCell-cell interactions influence all aspects of development, homeostasis, and disease. In cancer, interactions between cancer cells and stromal cells play a major role in nearly every step of carcinogenesis. Thus, the ability to record cell-cell interactions would facilitate mechanistic delineation of the role of the cancer microenvironment. Here, we describe GFP-based Touching Nexus (G-baToN) which relies upon nanobody-directed fluorescent protein transfer to enable sensitive and specific labeling of cells after cell-cell interactions. G-baToN is a generalizable system that enables physical contact-based labeling between various human and mouse cell types, including endothelial cell-pericyte, neuron-astrocyte, and diverse cancer-stromal cell pairs. A suite of orthogonal baToN tools enables reciprocal cell-cell labeling, interaction-dependent cargo transfer, and the identification of higher order cell-cell interactions across a wide range of cell types. The ability to track physically interacting cells with these simple and sensitive systems will greatly accelerate our understanding of the outputs of cell-cell interactions in cancer as well as across many biological processes.https://elifesciences.org/articles/61080cell-cell interactioncancer-stromalnanobodytumor microenvironment |
| spellingShingle | Rui Tang Christopher W Murray Ian L Linde Nicholas J Kramer Zhonglin Lyu Min K Tsai Leo C Chen Hongchen Cai Aaron D Gitler Edgar Engleman Wonjae Lee Monte M Winslow A versatile system to record cell-cell interactions eLife cell-cell interaction cancer-stromal nanobody tumor microenvironment |
| title | A versatile system to record cell-cell interactions |
| title_full | A versatile system to record cell-cell interactions |
| title_fullStr | A versatile system to record cell-cell interactions |
| title_full_unstemmed | A versatile system to record cell-cell interactions |
| title_short | A versatile system to record cell-cell interactions |
| title_sort | versatile system to record cell cell interactions |
| topic | cell-cell interaction cancer-stromal nanobody tumor microenvironment |
| url | https://elifesciences.org/articles/61080 |
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