Inhibitory Effect of Cold Atmospheric Plasma on Chronic Wound-Related Multispecies Biofilms
The presence of microbial biofilms in the wounds affects negatively the healing process and can contribute to therapeutic failures. This study aimed to establish the effective parameters of cold atmospheric plasma (CAP) against wound-related multispecies and monospecies biofilms, and to evaluate the...
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MDPI AG
2021-06-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/11/12/5441 |
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| author | Maria Alcionéia Carvalho de Oliveira Gabriela de Morais Gouvêa Lima Thalita M. Castaldelli Nishime Aline Vidal Lacerda Gontijo Beatriz Rossi Canuto de Menezes Marcelo Vidigal Caliari Konstantin Georgiev Kostov Cristiane Yumi Koga-Ito |
| author_facet | Maria Alcionéia Carvalho de Oliveira Gabriela de Morais Gouvêa Lima Thalita M. Castaldelli Nishime Aline Vidal Lacerda Gontijo Beatriz Rossi Canuto de Menezes Marcelo Vidigal Caliari Konstantin Georgiev Kostov Cristiane Yumi Koga-Ito |
| author_sort | Maria Alcionéia Carvalho de Oliveira |
| collection | DOAJ |
| description | The presence of microbial biofilms in the wounds affects negatively the healing process and can contribute to therapeutic failures. This study aimed to establish the effective parameters of cold atmospheric plasma (CAP) against wound-related multispecies and monospecies biofilms, and to evaluate the cytotoxicity and genotoxicity of the protocol. Monospecies and multispecies biofilms were formed by methicillin-resistant <i>Staphylococcus aureus</i> (MRSA), <i>Pseudomonas aeruginosa</i> and <i>Enterococcus faecalis</i>. The monospecies biofilms were grown in 96 wells plates and multispecies biofilm were formed on collagen membranes. The biofilms were exposed to helium CAP for 1, 3, 5 and 7 min. In monospecies biofilms, the inhibitory effect was detected after 1 min of exposure for <i>E. faecalis</i> and after 3 min for MRSA. A reduction in <i>P. aeruginosa</i> biofilm’s viability was detected after 7 min of exposure. For the multispecies biofilms, the reduction in the overall viability was detected after 5 min of exposure to CAP. Additionally, cytotoxicity and genotoxicity were evaluated by MTT assay and static cytometry, respectively. CAP showed low cytotoxicity and no genotoxicity to mouse fibroblastic cell line (3T3). It could be concluded that He-CAP showed inhibitory effect on wound-related multispecies biofilms, with low cytotoxicity and genotoxicity to mammalian cells. These findings point out the potential application of CAP in wound care. |
| first_indexed | 2024-03-10T10:28:59Z |
| format | Article |
| id | doaj.art-13a619d0929f4ef08bff38dd45375870 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T10:28:59Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-13a619d0929f4ef08bff38dd453758702023-11-21T23:46:30ZengMDPI AGApplied Sciences2076-34172021-06-011112544110.3390/app11125441Inhibitory Effect of Cold Atmospheric Plasma on Chronic Wound-Related Multispecies BiofilmsMaria Alcionéia Carvalho de Oliveira0Gabriela de Morais Gouvêa Lima1Thalita M. Castaldelli Nishime2Aline Vidal Lacerda Gontijo3Beatriz Rossi Canuto de Menezes4Marcelo Vidigal Caliari5Konstantin Georgiev Kostov6Cristiane Yumi Koga-Ito7Oral Biopathology Graduate Program, Institute of Science and Technology, São Paulo State University (UNESP), São José dos Campos 12245-000, SP, BrazilOral Biopathology Graduate Program, Institute of Science and Technology, São Paulo State University (UNESP), São José dos Campos 12245-000, SP, BrazilLeibniz Institute for Plasma Science and Technology, 17489 Greifswald, GermanyOral Biopathology Graduate Program, Institute of Science and Technology, São Paulo State University (UNESP), São José dos Campos 12245-000, SP, BrazilPlasmas and Process Laboratory (LPP), Technological Institute of Aeronautics (ITA), São José dos Campos 12228970, SP, BrazilDepartment of General Pathology, University Federal de Minas (UFMG), Belo Horizonte 12516-41, MG, BrazilDepartment of Physics, Guaratinguetá Faculty of Engineering, São Paulo State University (UNESP), Guaratinguetá 12516-410, SP, BrazilOral Biopathology Graduate Program, Institute of Science and Technology, São Paulo State University (UNESP), São José dos Campos 12245-000, SP, BrazilThe presence of microbial biofilms in the wounds affects negatively the healing process and can contribute to therapeutic failures. This study aimed to establish the effective parameters of cold atmospheric plasma (CAP) against wound-related multispecies and monospecies biofilms, and to evaluate the cytotoxicity and genotoxicity of the protocol. Monospecies and multispecies biofilms were formed by methicillin-resistant <i>Staphylococcus aureus</i> (MRSA), <i>Pseudomonas aeruginosa</i> and <i>Enterococcus faecalis</i>. The monospecies biofilms were grown in 96 wells plates and multispecies biofilm were formed on collagen membranes. The biofilms were exposed to helium CAP for 1, 3, 5 and 7 min. In monospecies biofilms, the inhibitory effect was detected after 1 min of exposure for <i>E. faecalis</i> and after 3 min for MRSA. A reduction in <i>P. aeruginosa</i> biofilm’s viability was detected after 7 min of exposure. For the multispecies biofilms, the reduction in the overall viability was detected after 5 min of exposure to CAP. Additionally, cytotoxicity and genotoxicity were evaluated by MTT assay and static cytometry, respectively. CAP showed low cytotoxicity and no genotoxicity to mouse fibroblastic cell line (3T3). It could be concluded that He-CAP showed inhibitory effect on wound-related multispecies biofilms, with low cytotoxicity and genotoxicity to mammalian cells. These findings point out the potential application of CAP in wound care.https://www.mdpi.com/2076-3417/11/12/5441woundsbiofilmplasma jet |
| spellingShingle | Maria Alcionéia Carvalho de Oliveira Gabriela de Morais Gouvêa Lima Thalita M. Castaldelli Nishime Aline Vidal Lacerda Gontijo Beatriz Rossi Canuto de Menezes Marcelo Vidigal Caliari Konstantin Georgiev Kostov Cristiane Yumi Koga-Ito Inhibitory Effect of Cold Atmospheric Plasma on Chronic Wound-Related Multispecies Biofilms Applied Sciences wounds biofilm plasma jet |
| title | Inhibitory Effect of Cold Atmospheric Plasma on Chronic Wound-Related Multispecies Biofilms |
| title_full | Inhibitory Effect of Cold Atmospheric Plasma on Chronic Wound-Related Multispecies Biofilms |
| title_fullStr | Inhibitory Effect of Cold Atmospheric Plasma on Chronic Wound-Related Multispecies Biofilms |
| title_full_unstemmed | Inhibitory Effect of Cold Atmospheric Plasma on Chronic Wound-Related Multispecies Biofilms |
| title_short | Inhibitory Effect of Cold Atmospheric Plasma on Chronic Wound-Related Multispecies Biofilms |
| title_sort | inhibitory effect of cold atmospheric plasma on chronic wound related multispecies biofilms |
| topic | wounds biofilm plasma jet |
| url | https://www.mdpi.com/2076-3417/11/12/5441 |
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