Light-induced dynamic RGD pattern for sequential modulation of macrophage phenotypes
Due to the critical roles of macrophage in immune response and tissue repair, harnessing macrophage phenotypes dynamically to match the tissue healing process on demand attracted many attentions. Although there have developed many advanced platforms with dynamic features for cell manipulation, few s...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2021-11-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X21001870 |
| _version_ | 1797203075565355008 |
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| author | Yilun Luo Xiaowen Zheng Peiqi Yuan Xingyao Ye Lie Ma |
| author_facet | Yilun Luo Xiaowen Zheng Peiqi Yuan Xingyao Ye Lie Ma |
| author_sort | Yilun Luo |
| collection | DOAJ |
| description | Due to the critical roles of macrophage in immune response and tissue repair, harnessing macrophage phenotypes dynamically to match the tissue healing process on demand attracted many attentions. Although there have developed many advanced platforms with dynamic features for cell manipulation, few studies have designed a dynamic chemical pattern to sequentially polarize macrophage phenotypes and meet the immune requirements at various tissue repair stages. Here, we propose a novel strategy for spatiotemporal manipulation of macrophage phenotypes by a UV-induced dynamic Arg-Gly-Asp (RGD) pattern. By employing a photo-patterning technique and the specific interaction between cyclodextrin (CD) and azobenzene-RGD (Azo-RGD), we prepared a polyethylene glycol-dithiol/polyethylene glycol-norbornene (PEG-SH/PEG-Nor) hydrogel with dynamic RGD-patterned surface. After irradiation with 365-nm UV light, the homogeneous RGD surface was transformed to the RGD-patterned surface which induced morphological transformation of macrophages from round to elongated and subsequent phenotypic transition from pro-inflammation to anti-inflammation. The mechanism of phenotypic polarization induced by RGD pattern was proved to be related to Rho-associated protein kinase 2 (ROCK2). Sequential modulation of macrophage phenotypes by the dynamic RGD-patterned surface provides a remote and non-invasive strategy to manipulate immune reactions and achieve optimized healing outcomes. |
| first_indexed | 2024-04-24T08:13:34Z |
| format | Article |
| id | doaj.art-13a7b3a79f594f0694e44aeab244a413 |
| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2024-04-24T08:13:34Z |
| publishDate | 2021-11-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj.art-13a7b3a79f594f0694e44aeab244a4132024-04-17T04:40:22ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2021-11-0161140654072Light-induced dynamic RGD pattern for sequential modulation of macrophage phenotypesYilun Luo0Xiaowen Zheng1Peiqi Yuan2Xingyao Ye3Lie Ma4MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, ChinaMOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, ChinaMOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, ChinaMOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, ChinaMOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China; Corresponding author.Due to the critical roles of macrophage in immune response and tissue repair, harnessing macrophage phenotypes dynamically to match the tissue healing process on demand attracted many attentions. Although there have developed many advanced platforms with dynamic features for cell manipulation, few studies have designed a dynamic chemical pattern to sequentially polarize macrophage phenotypes and meet the immune requirements at various tissue repair stages. Here, we propose a novel strategy for spatiotemporal manipulation of macrophage phenotypes by a UV-induced dynamic Arg-Gly-Asp (RGD) pattern. By employing a photo-patterning technique and the specific interaction between cyclodextrin (CD) and azobenzene-RGD (Azo-RGD), we prepared a polyethylene glycol-dithiol/polyethylene glycol-norbornene (PEG-SH/PEG-Nor) hydrogel with dynamic RGD-patterned surface. After irradiation with 365-nm UV light, the homogeneous RGD surface was transformed to the RGD-patterned surface which induced morphological transformation of macrophages from round to elongated and subsequent phenotypic transition from pro-inflammation to anti-inflammation. The mechanism of phenotypic polarization induced by RGD pattern was proved to be related to Rho-associated protein kinase 2 (ROCK2). Sequential modulation of macrophage phenotypes by the dynamic RGD-patterned surface provides a remote and non-invasive strategy to manipulate immune reactions and achieve optimized healing outcomes.http://www.sciencedirect.com/science/article/pii/S2452199X21001870Light-responsiveDynamic RGD patternMacrophage phenotypeImmune responseTissue repair |
| spellingShingle | Yilun Luo Xiaowen Zheng Peiqi Yuan Xingyao Ye Lie Ma Light-induced dynamic RGD pattern for sequential modulation of macrophage phenotypes Bioactive Materials Light-responsive Dynamic RGD pattern Macrophage phenotype Immune response Tissue repair |
| title | Light-induced dynamic RGD pattern for sequential modulation of macrophage phenotypes |
| title_full | Light-induced dynamic RGD pattern for sequential modulation of macrophage phenotypes |
| title_fullStr | Light-induced dynamic RGD pattern for sequential modulation of macrophage phenotypes |
| title_full_unstemmed | Light-induced dynamic RGD pattern for sequential modulation of macrophage phenotypes |
| title_short | Light-induced dynamic RGD pattern for sequential modulation of macrophage phenotypes |
| title_sort | light induced dynamic rgd pattern for sequential modulation of macrophage phenotypes |
| topic | Light-responsive Dynamic RGD pattern Macrophage phenotype Immune response Tissue repair |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X21001870 |
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