Optimized protocols for protoplast isolation, transfection, and regeneration in the Solanum genus for the CRISPR/Cas-mediated transgene-free genome editing

Optimized protocols for protoplast isolation, transfection, and regeneration in the Solanum genus for the CRISPR/Cas-mediated transgene-free genome editing

Abstract The Solanaceae family includes the largest flowering crops such as tomatoes, potatoes, and eggplants. Consumer demand has led to massive development of plants in the Solanum genus, and many different Solanum varieties are now available on the market. The recent advances in Clustered Regular...

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Bibliographic Details
Main Authors: So Hee Yang, Suk Weon Kim, Sujin Lee, Yeonjong Koo
Format: Article
Language:English
Published: SpringerOpen 2024-03-01
Series:Applied Biological Chemistry
Subjects:
Protoplast
Transient expression
Regeneration
Gene editing
CRISPR/Cas9
Online Access:https://doi.org/10.1186/s13765-024-00870-1

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