Using Microorganismal Consortium and Bioactive Substances to Treat Seeds of Two Scots Pine Ecotypes as a Technique to Increase Re-Afforestation Efficiency on Chalk Outcrops
The present research is focused on various pine ecotypes’ seed reproduction in the chalky substrate, challenging environmental conditions on the carbonate soils on chalk outcrops in the south of the Central Russian Upland in relation to pine woods re-afforestation. The winter and spring sowing metho...
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| Format: | Article |
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MDPI AG
2023-05-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/14/6/1093 |
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| author | Vladimir M. Kosolapov Vladmir I. Cherniavskih Elena V. Dumacheva Luiza D. Sajfutdinova Alexander A. Zhuchenko Alexey P. Glinushkin Helena V. Grishina Valery P. Kalinitchenko Svetlana V. Akimova Natalia A. Semenova Leonid V. Perelomov Svetlana V. Kozmenko |
| author_facet | Vladimir M. Kosolapov Vladmir I. Cherniavskih Elena V. Dumacheva Luiza D. Sajfutdinova Alexander A. Zhuchenko Alexey P. Glinushkin Helena V. Grishina Valery P. Kalinitchenko Svetlana V. Akimova Natalia A. Semenova Leonid V. Perelomov Svetlana V. Kozmenko |
| author_sort | Vladimir M. Kosolapov |
| collection | DOAJ |
| description | The present research is focused on various pine ecotypes’ seed reproduction in the chalky substrate, challenging environmental conditions on the carbonate soils on chalk outcrops in the south of the Central Russian Upland in relation to pine woods re-afforestation. The winter and spring sowing methods were studied, along with a pre-seeding treatment, by biopreparations based on a consortium of <i>Glomales</i> fungi, bacteria of the genus <i>Bacillus</i>, and bioactive substances. The seeds of two pine ecotypes, <i>Pinus sylvestris</i> L.; <i>Pinus sylvestris</i> var. <i>cretacea</i> Kalenicz exKom, underwent treatment. The study revealed that biopreparations and bioactive substances promote higher pine seed germination rates and ensure the stability and survivability of seedlings in an environment that is unfavorable for plant and tree ontogenesis. Applying biopreparations proved effective during spring sowing, whereas, in the case of winter sowing, their positive impact was not statistically significant. The net effect size of the three organized factors studied in the experiment (pine ecotype, biopreparation, sowing term) (h<sup>2</sup><sub>x</sub>) on the “survivability of <i>P. sylvestris</i> seedlings” effective feature significantly increased from 90.8 to 93.8%. The effect size of the “pine ecotype” factor on seedling survivability in <i>P. sylvestris</i> was at its highest (14.4%) during the seedlings’ first-year growth period. The effect size of the “sowing term” factor was at its highest (79.4%) at the stage of seed germination. The effect size of the “biopreparation” factor was at its highest (44.0%) during the seedlings’ second-year growth stage. Our results indicate that it is preferable to create forest plantations on chalk outcrops using <i>Pinus sylvestris</i> var. <i>cretacea</i> ecotypes and with pre-sowing seed treatment via biopreparations based on a microorganismal consortium and Biogor KM. The Spearman correlation between the nitrification capacity of soil substrate and seedling survivability during the first three growth periods (from planting till the next year’s springtime) was of a moderate size (r<sub>s</sub> = 0.617–0.673, <i>p</i> < 0.05). To improve the growth and productivity of young and mature Scots pine stands, a Biogeosystem Technique (BGT*) methodology was developed. |
| first_indexed | 2024-03-11T02:27:08Z |
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| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-11T02:27:08Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Forests |
| spelling | doaj.art-13aa6cb2de2f4a8084c732f387453f0e2023-11-18T10:26:18ZengMDPI AGForests1999-49072023-05-01146109310.3390/f14061093Using Microorganismal Consortium and Bioactive Substances to Treat Seeds of Two Scots Pine Ecotypes as a Technique to Increase Re-Afforestation Efficiency on Chalk OutcropsVladimir M. Kosolapov0Vladmir I. Cherniavskih1Elena V. Dumacheva2Luiza D. Sajfutdinova3Alexander A. Zhuchenko4Alexey P. Glinushkin5Helena V. Grishina6Valery P. Kalinitchenko7Svetlana V. Akimova8Natalia A. Semenova9Leonid V. Perelomov10Svetlana V. Kozmenko11Federal Williams Research Center of Forage Production and Agroecology, Nauczny Gorodok 1, 141055 Lobnya, RussiaFederal Williams Research Center of Forage Production and Agroecology, Nauczny Gorodok 1, 141055 Lobnya, RussiaFederal Williams Research Center of Forage Production and Agroecology, Nauczny Gorodok 1, 141055 Lobnya, RussiaFederal Williams Research Center of Forage Production and Agroecology, Nauczny Gorodok 1, 141055 Lobnya, RussiaAll-Russian Research Institute of Phytopathology, 143050 Moscow, RussiaAll-Russian Research Institute of Phytopathology, 143050 Moscow, RussiaAll-Russian Research Institute of Phytopathology, 143050 Moscow, RussiaAll-Russian Research Institute of Phytopathology, 143050 Moscow, RussiaAll-Russian Research Institute of Phytopathology, 143050 Moscow, RussiaFederal Scientific Agroengineering Center VIM, 1st Institutskiy Proezd, 109428 Moscow, RussiaLaboratory of Soil Chemistry and Ecology, Faculty of Natural Sciences, Tula State Lev Tolstoy Pedagogical University, Lenin Ave., 125, 300026 Tula, RussiaLaboratory of Soil Chemistry and Ecology, Faculty of Natural Sciences, Tula State Lev Tolstoy Pedagogical University, Lenin Ave., 125, 300026 Tula, RussiaThe present research is focused on various pine ecotypes’ seed reproduction in the chalky substrate, challenging environmental conditions on the carbonate soils on chalk outcrops in the south of the Central Russian Upland in relation to pine woods re-afforestation. The winter and spring sowing methods were studied, along with a pre-seeding treatment, by biopreparations based on a consortium of <i>Glomales</i> fungi, bacteria of the genus <i>Bacillus</i>, and bioactive substances. The seeds of two pine ecotypes, <i>Pinus sylvestris</i> L.; <i>Pinus sylvestris</i> var. <i>cretacea</i> Kalenicz exKom, underwent treatment. The study revealed that biopreparations and bioactive substances promote higher pine seed germination rates and ensure the stability and survivability of seedlings in an environment that is unfavorable for plant and tree ontogenesis. Applying biopreparations proved effective during spring sowing, whereas, in the case of winter sowing, their positive impact was not statistically significant. The net effect size of the three organized factors studied in the experiment (pine ecotype, biopreparation, sowing term) (h<sup>2</sup><sub>x</sub>) on the “survivability of <i>P. sylvestris</i> seedlings” effective feature significantly increased from 90.8 to 93.8%. The effect size of the “pine ecotype” factor on seedling survivability in <i>P. sylvestris</i> was at its highest (14.4%) during the seedlings’ first-year growth period. The effect size of the “sowing term” factor was at its highest (79.4%) at the stage of seed germination. The effect size of the “biopreparation” factor was at its highest (44.0%) during the seedlings’ second-year growth stage. Our results indicate that it is preferable to create forest plantations on chalk outcrops using <i>Pinus sylvestris</i> var. <i>cretacea</i> ecotypes and with pre-sowing seed treatment via biopreparations based on a microorganismal consortium and Biogor KM. The Spearman correlation between the nitrification capacity of soil substrate and seedling survivability during the first three growth periods (from planting till the next year’s springtime) was of a moderate size (r<sub>s</sub> = 0.617–0.673, <i>p</i> < 0.05). To improve the growth and productivity of young and mature Scots pine stands, a Biogeosystem Technique (BGT*) methodology was developed.https://www.mdpi.com/1999-4907/14/6/1093<i>Pinus sylvestris</i> var. <i>cretacea</i> Kalenicz. ex Kom.<i>Pinus sylvestris</i> L.artificial forest restoration (re-afforestation) by seedsowing termsbiopreparationsmicroorganismal consortium |
| spellingShingle | Vladimir M. Kosolapov Vladmir I. Cherniavskih Elena V. Dumacheva Luiza D. Sajfutdinova Alexander A. Zhuchenko Alexey P. Glinushkin Helena V. Grishina Valery P. Kalinitchenko Svetlana V. Akimova Natalia A. Semenova Leonid V. Perelomov Svetlana V. Kozmenko Using Microorganismal Consortium and Bioactive Substances to Treat Seeds of Two Scots Pine Ecotypes as a Technique to Increase Re-Afforestation Efficiency on Chalk Outcrops Forests <i>Pinus sylvestris</i> var. <i>cretacea</i> Kalenicz. ex Kom. <i>Pinus sylvestris</i> L. artificial forest restoration (re-afforestation) by seed sowing terms biopreparations microorganismal consortium |
| title | Using Microorganismal Consortium and Bioactive Substances to Treat Seeds of Two Scots Pine Ecotypes as a Technique to Increase Re-Afforestation Efficiency on Chalk Outcrops |
| title_full | Using Microorganismal Consortium and Bioactive Substances to Treat Seeds of Two Scots Pine Ecotypes as a Technique to Increase Re-Afforestation Efficiency on Chalk Outcrops |
| title_fullStr | Using Microorganismal Consortium and Bioactive Substances to Treat Seeds of Two Scots Pine Ecotypes as a Technique to Increase Re-Afforestation Efficiency on Chalk Outcrops |
| title_full_unstemmed | Using Microorganismal Consortium and Bioactive Substances to Treat Seeds of Two Scots Pine Ecotypes as a Technique to Increase Re-Afforestation Efficiency on Chalk Outcrops |
| title_short | Using Microorganismal Consortium and Bioactive Substances to Treat Seeds of Two Scots Pine Ecotypes as a Technique to Increase Re-Afforestation Efficiency on Chalk Outcrops |
| title_sort | using microorganismal consortium and bioactive substances to treat seeds of two scots pine ecotypes as a technique to increase re afforestation efficiency on chalk outcrops |
| topic | <i>Pinus sylvestris</i> var. <i>cretacea</i> Kalenicz. ex Kom. <i>Pinus sylvestris</i> L. artificial forest restoration (re-afforestation) by seed sowing terms biopreparations microorganismal consortium |
| url | https://www.mdpi.com/1999-4907/14/6/1093 |
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