Characterization of a novel class A carbapenemase PAD-1 from Paramesorhizobium desertii A-3-ET, a strain highly resistant to β-lactam antibiotics
Abstract Although clinical antibiotic-resistant bacteria have attracted tremendous attention in the microbiology community, the resistant bacteria that persist in natural environments have been overlooked for a longtime. We previously proposed a new species Paramesorhizobium desertii, isolated from...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-07841-1 |
| _version_ | 1819035964179218432 |
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| author | Ruichen Lv Jingyu Guo YanFeng Yan Rong Chen Lisheng Xiao Min Wang Nan Fang Chengxiang Fang Yujun Cui Ruifu Yang Yajun Song |
| author_facet | Ruichen Lv Jingyu Guo YanFeng Yan Rong Chen Lisheng Xiao Min Wang Nan Fang Chengxiang Fang Yujun Cui Ruifu Yang Yajun Song |
| author_sort | Ruichen Lv |
| collection | DOAJ |
| description | Abstract Although clinical antibiotic-resistant bacteria have attracted tremendous attention in the microbiology community, the resistant bacteria that persist in natural environments have been overlooked for a longtime. We previously proposed a new species Paramesorhizobium desertii, isolated from the soil of the Taklimakan Desert in China that is highly resistant to most β-lactam antibiotics. To identify potential β-lactamase(s) in this bacteria, we first confirmed the carbapenemase activity in the freeze–thawed supernatant of a P. desertii A-3-ET culture using the modified Hodge assay. We then identified a novel chromosome-encoded carbapenemase (PAD-1) in strain A-3-ET, using a shotgun proteomic analysis of the supernatant and genomic information. The bioinformatics analysis indicated that PAD-1 is a class A carbapenemase. Subsequent enzyme kinetic assays with purified PAD-1 confirmed its carbapenemase activity, which is similar to that of clinically significant class A carbapenemases, including BKC-1 and KPC-2. Because the location in which A-3-ET was isolated is not affected by human activity, PAD-1 is unlikely to be associated with the selection pressures exerted by modern antibiotics. This study confirmed the diversity of antibiotic-resistant determinants in the environmental resistome. |
| first_indexed | 2024-12-21T07:57:59Z |
| format | Article |
| id | doaj.art-13aac20497174f87aa96edd8e2638fa2 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-21T07:57:59Z |
| publishDate | 2017-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-13aac20497174f87aa96edd8e2638fa22022-12-21T19:10:56ZengNature PortfolioScientific Reports2045-23222017-08-01711910.1038/s41598-017-07841-1Characterization of a novel class A carbapenemase PAD-1 from Paramesorhizobium desertii A-3-ET, a strain highly resistant to β-lactam antibioticsRuichen Lv0Jingyu Guo1YanFeng Yan2Rong Chen3Lisheng Xiao4Min Wang5Nan Fang6Chengxiang Fang7Yujun Cui8Ruifu Yang9Yajun Song10State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and EpidemiologyState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and EpidemiologyState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and EpidemiologyState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and EpidemiologyState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and EpidemiologyState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and EpidemiologyState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and EpidemiologyCollege of Life Sciences, Wuhan UniversityState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and EpidemiologyState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and EpidemiologyState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and EpidemiologyAbstract Although clinical antibiotic-resistant bacteria have attracted tremendous attention in the microbiology community, the resistant bacteria that persist in natural environments have been overlooked for a longtime. We previously proposed a new species Paramesorhizobium desertii, isolated from the soil of the Taklimakan Desert in China that is highly resistant to most β-lactam antibiotics. To identify potential β-lactamase(s) in this bacteria, we first confirmed the carbapenemase activity in the freeze–thawed supernatant of a P. desertii A-3-ET culture using the modified Hodge assay. We then identified a novel chromosome-encoded carbapenemase (PAD-1) in strain A-3-ET, using a shotgun proteomic analysis of the supernatant and genomic information. The bioinformatics analysis indicated that PAD-1 is a class A carbapenemase. Subsequent enzyme kinetic assays with purified PAD-1 confirmed its carbapenemase activity, which is similar to that of clinically significant class A carbapenemases, including BKC-1 and KPC-2. Because the location in which A-3-ET was isolated is not affected by human activity, PAD-1 is unlikely to be associated with the selection pressures exerted by modern antibiotics. This study confirmed the diversity of antibiotic-resistant determinants in the environmental resistome.https://doi.org/10.1038/s41598-017-07841-1 |
| spellingShingle | Ruichen Lv Jingyu Guo YanFeng Yan Rong Chen Lisheng Xiao Min Wang Nan Fang Chengxiang Fang Yujun Cui Ruifu Yang Yajun Song Characterization of a novel class A carbapenemase PAD-1 from Paramesorhizobium desertii A-3-ET, a strain highly resistant to β-lactam antibiotics Scientific Reports |
| title | Characterization of a novel class A carbapenemase PAD-1 from Paramesorhizobium desertii A-3-ET, a strain highly resistant to β-lactam antibiotics |
| title_full | Characterization of a novel class A carbapenemase PAD-1 from Paramesorhizobium desertii A-3-ET, a strain highly resistant to β-lactam antibiotics |
| title_fullStr | Characterization of a novel class A carbapenemase PAD-1 from Paramesorhizobium desertii A-3-ET, a strain highly resistant to β-lactam antibiotics |
| title_full_unstemmed | Characterization of a novel class A carbapenemase PAD-1 from Paramesorhizobium desertii A-3-ET, a strain highly resistant to β-lactam antibiotics |
| title_short | Characterization of a novel class A carbapenemase PAD-1 from Paramesorhizobium desertii A-3-ET, a strain highly resistant to β-lactam antibiotics |
| title_sort | characterization of a novel class a carbapenemase pad 1 from paramesorhizobium desertii a 3 et a strain highly resistant to β lactam antibiotics |
| url | https://doi.org/10.1038/s41598-017-07841-1 |
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