Differentiation Capacity of Porcine Skeletal Muscle-Derived Stem Cells as Intermediate Species between Mice and Humans
Large animal experiments are important for preclinical studies of regenerative stem cell transplantation therapy. Therefore, we investigated the differentiation capacity of pig skeletal muscle-derived stem cells (Sk-MSCs) as an intermediate model between mice and humans for nerve muscle regenerative...
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MDPI AG
2023-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/12/9862 |
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| author | Tetsuro Tamaki Toshiharu Natsume Akira Katoh Nobuyuki Nakajima Kosuke Saito Tsuyoshi Fukuzawa Masayoshi Otake Satoko Enya Akihisa Kangawa Takeshi Imai Miyu Tamaki Yoshiyasu Uchiyama |
| author_facet | Tetsuro Tamaki Toshiharu Natsume Akira Katoh Nobuyuki Nakajima Kosuke Saito Tsuyoshi Fukuzawa Masayoshi Otake Satoko Enya Akihisa Kangawa Takeshi Imai Miyu Tamaki Yoshiyasu Uchiyama |
| author_sort | Tetsuro Tamaki |
| collection | DOAJ |
| description | Large animal experiments are important for preclinical studies of regenerative stem cell transplantation therapy. Therefore, we investigated the differentiation capacity of pig skeletal muscle-derived stem cells (Sk-MSCs) as an intermediate model between mice and humans for nerve muscle regenerative therapy. Enzymatically extracted cells were obtained from green-fluorescence transgenic micro-mini pigs (GFP-Tg MMP) and sorted as CD34+/45− (Sk-34) and CD34−/45−/29+ (Sk-DN) fractions. The ability to differentiate into skeletal muscle, peripheral nerve, and vascular cell lineages was examined via in vitro cell culture and in vivo cell transplantation into the damaged tibialis anterior muscle and sciatic nerves of nude mice and rats. Protein and mRNA levels were analyzed using RT-PCR, immunohistochemistry, and immunoelectron microscopy. The myogenic potential, which was tested by Pax7 and MyoD expression and the formation of muscle fibers, was higher in Sk-DN cells than in Sk-34 cells but remained weak in the latter. In contrast, the capacity to differentiate into peripheral nerve and vascular cell lineages was significantly stronger in Sk-34 cells. In particular, Sk-DN cells did not engraft to the damaged nerve, whereas Sk-34 cells showed active engraftment and differentiation into perineurial/endoneurial cells, endothelial cells, and vascular smooth muscle cells, similar to the human case, as previously reported. Therefore, we concluded that Sk-34 and Sk-DN cells in pigs are closer to those in humans than to those in mice. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T02:22:05Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-13abf02eb7ad4b2b975dd971cc9b34882023-11-18T10:45:11ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-06-012412986210.3390/ijms24129862Differentiation Capacity of Porcine Skeletal Muscle-Derived Stem Cells as Intermediate Species between Mice and HumansTetsuro Tamaki0Toshiharu Natsume1Akira Katoh2Nobuyuki Nakajima3Kosuke Saito4Tsuyoshi Fukuzawa5Masayoshi Otake6Satoko Enya7Akihisa Kangawa8Takeshi Imai9Miyu Tamaki10Yoshiyasu Uchiyama11Muscle Physiology and Cell Biology Unit, Tokai University School of Medicine, 143 Shimokasuya, Isehara 259-1193, JapanMuscle Physiology and Cell Biology Unit, Tokai University School of Medicine, 143 Shimokasuya, Isehara 259-1193, JapanMuscle Physiology and Cell Biology Unit, Tokai University School of Medicine, 143 Shimokasuya, Isehara 259-1193, JapanMuscle Physiology and Cell Biology Unit, Tokai University School of Medicine, 143 Shimokasuya, Isehara 259-1193, JapanMuscle Physiology and Cell Biology Unit, Tokai University School of Medicine, 143 Shimokasuya, Isehara 259-1193, JapanMuscle Physiology and Cell Biology Unit, Tokai University School of Medicine, 143 Shimokasuya, Isehara 259-1193, JapanSwine and Poultry Research Center, Shizuoka Prefectural Research Institute of Animal Industry, 2780 Nishikata, Kikugawa 439-0037, JapanSwine and Poultry Research Center, Shizuoka Prefectural Research Institute of Animal Industry, 2780 Nishikata, Kikugawa 439-0037, JapanSwine and Poultry Research Center, Shizuoka Prefectural Research Institute of Animal Industry, 2780 Nishikata, Kikugawa 439-0037, JapanMuscle Physiology and Cell Biology Unit, Tokai University School of Medicine, 143 Shimokasuya, Isehara 259-1193, JapanMuscle Physiology and Cell Biology Unit, Tokai University School of Medicine, 143 Shimokasuya, Isehara 259-1193, JapanMuscle Physiology and Cell Biology Unit, Tokai University School of Medicine, 143 Shimokasuya, Isehara 259-1193, JapanLarge animal experiments are important for preclinical studies of regenerative stem cell transplantation therapy. Therefore, we investigated the differentiation capacity of pig skeletal muscle-derived stem cells (Sk-MSCs) as an intermediate model between mice and humans for nerve muscle regenerative therapy. Enzymatically extracted cells were obtained from green-fluorescence transgenic micro-mini pigs (GFP-Tg MMP) and sorted as CD34+/45− (Sk-34) and CD34−/45−/29+ (Sk-DN) fractions. The ability to differentiate into skeletal muscle, peripheral nerve, and vascular cell lineages was examined via in vitro cell culture and in vivo cell transplantation into the damaged tibialis anterior muscle and sciatic nerves of nude mice and rats. Protein and mRNA levels were analyzed using RT-PCR, immunohistochemistry, and immunoelectron microscopy. The myogenic potential, which was tested by Pax7 and MyoD expression and the formation of muscle fibers, was higher in Sk-DN cells than in Sk-34 cells but remained weak in the latter. In contrast, the capacity to differentiate into peripheral nerve and vascular cell lineages was significantly stronger in Sk-34 cells. In particular, Sk-DN cells did not engraft to the damaged nerve, whereas Sk-34 cells showed active engraftment and differentiation into perineurial/endoneurial cells, endothelial cells, and vascular smooth muscle cells, similar to the human case, as previously reported. Therefore, we concluded that Sk-34 and Sk-DN cells in pigs are closer to those in humans than to those in mice.https://www.mdpi.com/1422-0067/24/12/9862micro-mini piglarge animal experimentGFP-transgenic pigmultipotent stem cellsskeletal musclenerve-muscle regeneration |
| spellingShingle | Tetsuro Tamaki Toshiharu Natsume Akira Katoh Nobuyuki Nakajima Kosuke Saito Tsuyoshi Fukuzawa Masayoshi Otake Satoko Enya Akihisa Kangawa Takeshi Imai Miyu Tamaki Yoshiyasu Uchiyama Differentiation Capacity of Porcine Skeletal Muscle-Derived Stem Cells as Intermediate Species between Mice and Humans International Journal of Molecular Sciences micro-mini pig large animal experiment GFP-transgenic pig multipotent stem cells skeletal muscle nerve-muscle regeneration |
| title | Differentiation Capacity of Porcine Skeletal Muscle-Derived Stem Cells as Intermediate Species between Mice and Humans |
| title_full | Differentiation Capacity of Porcine Skeletal Muscle-Derived Stem Cells as Intermediate Species between Mice and Humans |
| title_fullStr | Differentiation Capacity of Porcine Skeletal Muscle-Derived Stem Cells as Intermediate Species between Mice and Humans |
| title_full_unstemmed | Differentiation Capacity of Porcine Skeletal Muscle-Derived Stem Cells as Intermediate Species between Mice and Humans |
| title_short | Differentiation Capacity of Porcine Skeletal Muscle-Derived Stem Cells as Intermediate Species between Mice and Humans |
| title_sort | differentiation capacity of porcine skeletal muscle derived stem cells as intermediate species between mice and humans |
| topic | micro-mini pig large animal experiment GFP-transgenic pig multipotent stem cells skeletal muscle nerve-muscle regeneration |
| url | https://www.mdpi.com/1422-0067/24/12/9862 |
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