A structural overview of GH61 proteins – fungal cellulose degrading polysaccharide monooxygenases
Recent years have witnessed a spurt of activities in the elucidation of the molecular function of a class of proteins with great potential in biomass degradation. GH61 proteins are of fungal origin and were originally classified in family 61 of the glycoside hydrolases. From the beginning they were...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2012-09-01
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| Series: | Computational and Structural Biotechnology Journal |
| Online Access: | http://journals.sfu.ca/rncsb/index.php/csbj/article/view/csbj.201209019 |
| _version_ | 1818274029133365248 |
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| author | Leila Lo Leggio Ditte Welner Leonardo De Maria |
| author_facet | Leila Lo Leggio Ditte Welner Leonardo De Maria |
| author_sort | Leila Lo Leggio |
| collection | DOAJ |
| description | Recent years have witnessed a spurt of activities in the elucidation of the molecular function of a class of proteins with great potential in biomass degradation. GH61 proteins are of fungal origin and were originally classified in family 61 of the glycoside hydrolases. From the beginning they were strongly suspected to be involved in cellulose degradation because of their expression profiles, despite very low detectable endoglucanase activities. A major breakthrough came from structure determination of the first members, establishing the presence of a divalent metal binding site and a similarity to bacterial proteins involved in chitin degradation. A second breakthrough came from the identification of cellulase boosting activity dependent on the integrity of the metal binding site. Finally very recently GH61 proteins were demonstrated to oxidatively cleave crystalline cellulose in a Cu and reductant dependant manner. This mini-review in particular focuses on the contribution that structure elucidation has made in the understanding of GH61 molecular function and reviews the currently known structures and the challenges remaining ahead for exploiting this new class of enzymes to the full. |
| first_indexed | 2024-12-12T22:07:21Z |
| format | Article |
| id | doaj.art-13ac95aa99454290b209ddbdede19759 |
| institution | Directory Open Access Journal |
| issn | 2001-0370 |
| language | English |
| last_indexed | 2024-12-12T22:07:21Z |
| publishDate | 2012-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Computational and Structural Biotechnology Journal |
| spelling | doaj.art-13ac95aa99454290b209ddbdede197592022-12-22T00:10:21ZengElsevierComputational and Structural Biotechnology Journal2001-03702012-09-0123e201209019A structural overview of GH61 proteins – fungal cellulose degrading polysaccharide monooxygenasesLeila Lo LeggioDitte WelnerLeonardo De MariaRecent years have witnessed a spurt of activities in the elucidation of the molecular function of a class of proteins with great potential in biomass degradation. GH61 proteins are of fungal origin and were originally classified in family 61 of the glycoside hydrolases. From the beginning they were strongly suspected to be involved in cellulose degradation because of their expression profiles, despite very low detectable endoglucanase activities. A major breakthrough came from structure determination of the first members, establishing the presence of a divalent metal binding site and a similarity to bacterial proteins involved in chitin degradation. A second breakthrough came from the identification of cellulase boosting activity dependent on the integrity of the metal binding site. Finally very recently GH61 proteins were demonstrated to oxidatively cleave crystalline cellulose in a Cu and reductant dependant manner. This mini-review in particular focuses on the contribution that structure elucidation has made in the understanding of GH61 molecular function and reviews the currently known structures and the challenges remaining ahead for exploiting this new class of enzymes to the full.http://journals.sfu.ca/rncsb/index.php/csbj/article/view/csbj.201209019 |
| spellingShingle | Leila Lo Leggio Ditte Welner Leonardo De Maria A structural overview of GH61 proteins – fungal cellulose degrading polysaccharide monooxygenases Computational and Structural Biotechnology Journal |
| title | A structural overview of GH61 proteins – fungal cellulose degrading polysaccharide monooxygenases |
| title_full | A structural overview of GH61 proteins – fungal cellulose degrading polysaccharide monooxygenases |
| title_fullStr | A structural overview of GH61 proteins – fungal cellulose degrading polysaccharide monooxygenases |
| title_full_unstemmed | A structural overview of GH61 proteins – fungal cellulose degrading polysaccharide monooxygenases |
| title_short | A structural overview of GH61 proteins – fungal cellulose degrading polysaccharide monooxygenases |
| title_sort | structural overview of gh61 proteins fungal cellulose degrading polysaccharide monooxygenases |
| url | http://journals.sfu.ca/rncsb/index.php/csbj/article/view/csbj.201209019 |
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