Field experiment on effect of butaphosphan and cyanocobalamin complex on the immunity and stress of olive flounder at low temperature
In this study, a fish metabolic accelerator (a combination of butaphosphan and cyanocobalamin [BPC]) was injected into the muscle of the olive flounder, Paralichthys olivaceus, to investigate its effect on immunity and stress in fish ma...
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| Format: | Article |
| Language: | English |
| Published: |
The Korean Society of Fisheries and Aquatic Science
2021-04-01
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| Series: | Fisheries and Aquatic Sciences |
| Subjects: | |
| Online Access: | http://www.e-fas.org/archive/view_article?pid=fas-24-4-153 |
| _version_ | 1818156249419612160 |
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| author | Seung Min Kim Da Won Lee You Jeong Kim Lyu Jin Jun Hyun Kyung Park Ye Ji Kim You Yong Jeong Sung Ho Lee Mun Gyeong Kwon Joon Bum Jeong |
| author_facet | Seung Min Kim Da Won Lee You Jeong Kim Lyu Jin Jun Hyun Kyung Park Ye Ji Kim You Yong Jeong Sung Ho Lee Mun Gyeong Kwon Joon Bum Jeong |
| author_sort | Seung Min Kim |
| collection | DOAJ |
| description | In this study, a fish metabolic accelerator (a combination of butaphosphan and
cyanocobalamin [BPC]) was injected into the muscle of the olive flounder,
Paralichthys olivaceus, to investigate its effect on
immunity and stress in fish maintained at low temperatures. A single dose of BPC
was injected (100 mg/kg body weight) into the olive flounder, and its immunity
and stress were observed after one and two weeks. Immunity tests revealed the
presence of lysozyme (LZM), nitroblue tetrazolium (NBT), myeloperoxidase (MPO),
anti-protease (AP), glutathione peroxidase (GPx), and total immunoglobulin
(TIg). BPC injection was found to increase immunity activity compared to the
control group. In particular, there was significantly high GPx activity. There
was similarly high activity for MPO and GPx in the first week following the
injection, followed by significant differences between the BPC-injected and
control groups in the second week. There was a reduced low water-temperature
stress response in the BPC-injected fish, as evidenced by the cortisol and
glucose levels of the control and BPC groups. Lower levels were also observed in
the BPC group than the control group during the second week. Cortisol levels
were significantly lower in the BPC group than the control group. Histological
examinations were conducted in the first and second weeks after the
intramuscular injection of the recommended BPC dose to confirm the safety of BPC
in aquaculture. There were no abnormalities observed in any tissue samples. This
study confirms that the injection of BPC is safe even when used in a culture
situation. BPC helps relieve stress and improves non-specific immune responses
(innate immunity) in the olive flounder. |
| first_indexed | 2024-12-11T14:55:18Z |
| format | Article |
| id | doaj.art-13aeea857fc7486cae72b6a6c296d8df |
| institution | Directory Open Access Journal |
| issn | 2234-1757 |
| language | English |
| last_indexed | 2024-12-11T14:55:18Z |
| publishDate | 2021-04-01 |
| publisher | The Korean Society of Fisheries and Aquatic Science |
| record_format | Article |
| series | Fisheries and Aquatic Sciences |
| spelling | doaj.art-13aeea857fc7486cae72b6a6c296d8df2022-12-22T01:01:19ZengThe Korean Society of Fisheries and Aquatic ScienceFisheries and Aquatic Sciences2234-17572021-04-0124415316210.47853/FAS.2021.e15fas-24-4-153Field experiment on effect of butaphosphan and cyanocobalamin complex on the immunity and stress of olive flounder at low temperatureSeung Min Kim0Da Won Lee1You Jeong Kim2Lyu Jin Jun3Hyun Kyung Park4Ye Ji Kim5You Yong Jeong6Sung Ho Lee7Mun Gyeong Kwon8Joon Bum Jeong9Mokpo Regional Office, National Fishery Products Quality Management, Mokpo 58746, KoreaAquatic Life Disease Control Division, National Fishery Products Quality Management, Busan 49111, KoreaCentral Research Institute, Woogen B&G, Hwaseong 18629, KoreaDepartment of Aquatic Biomedical Science, Jeju National University, Jeju 63243, KoreaDepartment of Aquatic Biomedical Science, Jeju National University, Jeju 63243, KoreaDepartment of Aquatic Biomedical Science, Jeju National University, Jeju 63243, KoreaCentral Research Institute, Woogen B&G, Hwaseong 18629, KoreaCentral Research Institute, Woogen B&G, Hwaseong 18629, KoreaAquatic Life Disease Control Division, National Fishery Products Quality Management, Busan 49111, KoreaDepartment of Aquatic Biomedical Science, Jeju National University, Jeju 63243, KoreaIn this study, a fish metabolic accelerator (a combination of butaphosphan and cyanocobalamin [BPC]) was injected into the muscle of the olive flounder, Paralichthys olivaceus, to investigate its effect on immunity and stress in fish maintained at low temperatures. A single dose of BPC was injected (100 mg/kg body weight) into the olive flounder, and its immunity and stress were observed after one and two weeks. Immunity tests revealed the presence of lysozyme (LZM), nitroblue tetrazolium (NBT), myeloperoxidase (MPO), anti-protease (AP), glutathione peroxidase (GPx), and total immunoglobulin (TIg). BPC injection was found to increase immunity activity compared to the control group. In particular, there was significantly high GPx activity. There was similarly high activity for MPO and GPx in the first week following the injection, followed by significant differences between the BPC-injected and control groups in the second week. There was a reduced low water-temperature stress response in the BPC-injected fish, as evidenced by the cortisol and glucose levels of the control and BPC groups. Lower levels were also observed in the BPC group than the control group during the second week. Cortisol levels were significantly lower in the BPC group than the control group. Histological examinations were conducted in the first and second weeks after the intramuscular injection of the recommended BPC dose to confirm the safety of BPC in aquaculture. There were no abnormalities observed in any tissue samples. This study confirms that the injection of BPC is safe even when used in a culture situation. BPC helps relieve stress and improves non-specific immune responses (innate immunity) in the olive flounder.http://www.e-fas.org/archive/view_article?pid=fas-24-4-153butaphosphancyanocobalaminimmunityolive flounderstress |
| spellingShingle | Seung Min Kim Da Won Lee You Jeong Kim Lyu Jin Jun Hyun Kyung Park Ye Ji Kim You Yong Jeong Sung Ho Lee Mun Gyeong Kwon Joon Bum Jeong Field experiment on effect of butaphosphan and cyanocobalamin complex on the immunity and stress of olive flounder at low temperature Fisheries and Aquatic Sciences butaphosphan cyanocobalamin immunity olive flounder stress |
| title | Field experiment on effect of butaphosphan and cyanocobalamin complex
on the immunity and stress of olive flounder at low temperature |
| title_full | Field experiment on effect of butaphosphan and cyanocobalamin complex
on the immunity and stress of olive flounder at low temperature |
| title_fullStr | Field experiment on effect of butaphosphan and cyanocobalamin complex
on the immunity and stress of olive flounder at low temperature |
| title_full_unstemmed | Field experiment on effect of butaphosphan and cyanocobalamin complex
on the immunity and stress of olive flounder at low temperature |
| title_short | Field experiment on effect of butaphosphan and cyanocobalamin complex
on the immunity and stress of olive flounder at low temperature |
| title_sort | field experiment on effect of butaphosphan and cyanocobalamin complex on the immunity and stress of olive flounder at low temperature |
| topic | butaphosphan cyanocobalamin immunity olive flounder stress |
| url | http://www.e-fas.org/archive/view_article?pid=fas-24-4-153 |
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