The Therapeutic Effect and the Possible Mechanism of C-Phycocyanin in Lipopolysaccharide and Seawater-Induced Acute Lung Injury
Leifang Zhang,1 Deyi Kong,1 Junxia Huang,1 Qiongfen Wang,2 Lilin Shao1 1Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, 316022, People’s Republic of China; 2Zhoushan Institute of Calibration a...
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Dove Medical Press
2022-04-01
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| Series: | Drug Design, Development and Therapy |
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| Online Access: | https://www.dovepress.com/the-therapeutic-effect-and-the-possible-mechanism-of-c-phycocyanin-in--peer-reviewed-fulltext-article-DDDT |
| _version_ | 1811284646075301888 |
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| author | Zhang L Kong D Huang J Wang Q Shao L |
| author_facet | Zhang L Kong D Huang J Wang Q Shao L |
| author_sort | Zhang L |
| collection | DOAJ |
| description | Leifang Zhang,1 Deyi Kong,1 Junxia Huang,1 Qiongfen Wang,2 Lilin Shao1 1Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, 316022, People’s Republic of China; 2Zhoushan Institute of Calibration and Testing for Quality and Technical Supervision, Zhoushan, 316012, Zhejiang, People’s Republic of ChinaCorrespondence: Leifang Zhang, Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, 1 Haida South Road, Changzhi Island, Zhoushan, 316022, Zhejiang, People’s Republic of China, Tel +86 18 8570 63534, Fax +86 58 0 254 781, Email zhanglf@zjou.edu.cnBackground: Seawater drowning-induced acute lung injury (ALI) is a severe clinical condition characterized by increased alveolar-capillary permeability, excessive inflammatory response, and refractory hypoxemia. C-phycocyanin (C-PC), a biliprotein found in blue-green algae such as spirulina platensis, is widely used in the food and dietary nutritional supplement fields due to its beneficial pharmacological effects. Previous studies have revealed that C-PC has anti-inflammatory, antioxidant, and anti-apoptotic activities.Purpose: Therefore, this study investigated the protective effect and underlying mechanisms of C-PC on lipopolysaccharide (LPS) and seawater (SW) induced ALI (SW and LPS-induced ALI).Methods: An SW and LPS mouse model of ALI mice was established through intratracheal administration of 5mg/kg LPS and 25% SW. Different doses of C-PC (100, 200 and 400 mg/kg) were administered by intraperitoneal injection for seven days. In addition, gap junction communication in RAW264.7 and MLE-12 cells was determined following stimulation with 25% SW and 10 μg/ml LPS after treatment with C-PC (120 μg/ml). Moreover, the arterial partial pressure of oxygen, lung wet/dry weight ratios, total protein content and MPO levels in the bronchoalveolar lavage fluid (BALF), and the histopathologic and ultrastructure staining of the lung tissues were determined. The oxidative stress index, levels of the pro-inflammatory mediators, epithelial cell viability and apoptosis, and the regulatory effect of C-PC on the NF-κB/NLRP3 axis were investigated.Results: The results showed that C-PC significantly alleviated pathological damages, suppressed oxidative stress, inflammation and apoptosis, and enhanced the viability of epithelial cells in the lung tissues. Furthermore, C-PC was shown to inhibit activation of the NF-κB/NLRP3 pathway and the formation of the NLRP3 inflammasome complex.Conclusions: In conclusion, C-PC shows promising therapeutic value in SW and LPS-induced ALI/ARDS, providing new insight into ALI/ARDS treatment.Keywords: C-phycocyanin, acute lung injury, inflammation, NF-κB/NLRP3, apoptosis |
| first_indexed | 2024-04-13T02:32:43Z |
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| issn | 1177-8881 |
| language | English |
| last_indexed | 2024-04-13T02:32:43Z |
| publishDate | 2022-04-01 |
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| series | Drug Design, Development and Therapy |
| spelling | doaj.art-13b1065164844ccd96a7e62de71fc6942022-12-22T03:06:30ZengDove Medical PressDrug Design, Development and Therapy1177-88812022-04-01Volume 161025104074262The Therapeutic Effect and the Possible Mechanism of C-Phycocyanin in Lipopolysaccharide and Seawater-Induced Acute Lung InjuryZhang LKong DHuang JWang QShao LLeifang Zhang,1 Deyi Kong,1 Junxia Huang,1 Qiongfen Wang,2 Lilin Shao1 1Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, 316022, People’s Republic of China; 2Zhoushan Institute of Calibration and Testing for Quality and Technical Supervision, Zhoushan, 316012, Zhejiang, People’s Republic of ChinaCorrespondence: Leifang Zhang, Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, 1 Haida South Road, Changzhi Island, Zhoushan, 316022, Zhejiang, People’s Republic of China, Tel +86 18 8570 63534, Fax +86 58 0 254 781, Email zhanglf@zjou.edu.cnBackground: Seawater drowning-induced acute lung injury (ALI) is a severe clinical condition characterized by increased alveolar-capillary permeability, excessive inflammatory response, and refractory hypoxemia. C-phycocyanin (C-PC), a biliprotein found in blue-green algae such as spirulina platensis, is widely used in the food and dietary nutritional supplement fields due to its beneficial pharmacological effects. Previous studies have revealed that C-PC has anti-inflammatory, antioxidant, and anti-apoptotic activities.Purpose: Therefore, this study investigated the protective effect and underlying mechanisms of C-PC on lipopolysaccharide (LPS) and seawater (SW) induced ALI (SW and LPS-induced ALI).Methods: An SW and LPS mouse model of ALI mice was established through intratracheal administration of 5mg/kg LPS and 25% SW. Different doses of C-PC (100, 200 and 400 mg/kg) were administered by intraperitoneal injection for seven days. In addition, gap junction communication in RAW264.7 and MLE-12 cells was determined following stimulation with 25% SW and 10 μg/ml LPS after treatment with C-PC (120 μg/ml). Moreover, the arterial partial pressure of oxygen, lung wet/dry weight ratios, total protein content and MPO levels in the bronchoalveolar lavage fluid (BALF), and the histopathologic and ultrastructure staining of the lung tissues were determined. The oxidative stress index, levels of the pro-inflammatory mediators, epithelial cell viability and apoptosis, and the regulatory effect of C-PC on the NF-κB/NLRP3 axis were investigated.Results: The results showed that C-PC significantly alleviated pathological damages, suppressed oxidative stress, inflammation and apoptosis, and enhanced the viability of epithelial cells in the lung tissues. Furthermore, C-PC was shown to inhibit activation of the NF-κB/NLRP3 pathway and the formation of the NLRP3 inflammasome complex.Conclusions: In conclusion, C-PC shows promising therapeutic value in SW and LPS-induced ALI/ARDS, providing new insight into ALI/ARDS treatment.Keywords: C-phycocyanin, acute lung injury, inflammation, NF-κB/NLRP3, apoptosishttps://www.dovepress.com/the-therapeutic-effect-and-the-possible-mechanism-of-c-phycocyanin-in--peer-reviewed-fulltext-article-DDDTc-phycocyaninacute lung injuryinflammationnf-κb/nlrp3apoptosis |
| spellingShingle | Zhang L Kong D Huang J Wang Q Shao L The Therapeutic Effect and the Possible Mechanism of C-Phycocyanin in Lipopolysaccharide and Seawater-Induced Acute Lung Injury Drug Design, Development and Therapy c-phycocyanin acute lung injury inflammation nf-κb/nlrp3 apoptosis |
| title | The Therapeutic Effect and the Possible Mechanism of C-Phycocyanin in Lipopolysaccharide and Seawater-Induced Acute Lung Injury |
| title_full | The Therapeutic Effect and the Possible Mechanism of C-Phycocyanin in Lipopolysaccharide and Seawater-Induced Acute Lung Injury |
| title_fullStr | The Therapeutic Effect and the Possible Mechanism of C-Phycocyanin in Lipopolysaccharide and Seawater-Induced Acute Lung Injury |
| title_full_unstemmed | The Therapeutic Effect and the Possible Mechanism of C-Phycocyanin in Lipopolysaccharide and Seawater-Induced Acute Lung Injury |
| title_short | The Therapeutic Effect and the Possible Mechanism of C-Phycocyanin in Lipopolysaccharide and Seawater-Induced Acute Lung Injury |
| title_sort | therapeutic effect and the possible mechanism of c phycocyanin in lipopolysaccharide and seawater induced acute lung injury |
| topic | c-phycocyanin acute lung injury inflammation nf-κb/nlrp3 apoptosis |
| url | https://www.dovepress.com/the-therapeutic-effect-and-the-possible-mechanism-of-c-phycocyanin-in--peer-reviewed-fulltext-article-DDDT |
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