Transcriptomic Analysis of Gonadal Adipose Tissue in Male Mice Exposed Perinatally to 2,2′,4,4′-Tetrabromodiphenyl Ether (BDE-47)
For the majority of lipophilic compounds, adipose tissue is traditionally considered as a storage depot and only rarely as a target organ. Meanwhile, abnormalities in adipose tissue physiology induced by chemical exposure may contribute to the current epidemic of obesity and metabolic diseases. Poly...
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MDPI AG
2018-03-01
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| Series: | Toxics |
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| Online Access: | http://www.mdpi.com/2305-6304/6/2/21 |
| _version_ | 1811335790840512512 |
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| author | Aser Abrha Alexander Suvorov |
| author_facet | Aser Abrha Alexander Suvorov |
| author_sort | Aser Abrha |
| collection | DOAJ |
| description | For the majority of lipophilic compounds, adipose tissue is traditionally considered as a storage depot and only rarely as a target organ. Meanwhile, abnormalities in adipose tissue physiology induced by chemical exposure may contribute to the current epidemic of obesity and metabolic diseases. Polybrominated diphenyl ethers (PBDEs) are a group of lipophilic flame retardants found in the majority of human samples in North America. Their ability to alter the physiology of adipose tissue is unknown. We exposed pregnant mice to 0.2 mg/kg body weight/day of BDE-47 perinatally. Transcriptomic changes in gonadal adipose tissue were analyzed in male offspring using the RNA-seq approach with subsequent bioinformatic analysis. The expression of genes of coagulation and complement cascade, de novo lipogenesis, and xenobiotic metabolism was altered in response to BDE-47 exposure. The affected molecular network included the following hubs: PPARα, HNF1A, and HNF4. These findings suggest that adipose tissue should be considered a target tissue for BDE-47, in addition to its role as a storage depot. This study also builds a background for a targeted search of sensitive phenotypic endpoints of BDE-47 exposure, including lipid profile parameters and coagulation factors in circulation. Additional studies are needed to investigate the role of PBDEs as an obesogen. |
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| institution | Directory Open Access Journal |
| issn | 2305-6304 |
| language | English |
| last_indexed | 2024-04-13T17:30:14Z |
| publishDate | 2018-03-01 |
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| spelling | doaj.art-13b16541980e412fb1d9c19911ed764c2022-12-22T02:37:36ZengMDPI AGToxics2305-63042018-03-01622110.3390/toxics6020021toxics6020021Transcriptomic Analysis of Gonadal Adipose Tissue in Male Mice Exposed Perinatally to 2,2′,4,4′-Tetrabromodiphenyl Ether (BDE-47)Aser Abrha0Alexander Suvorov1Department of Environmental Health Sciences, University of Massachusetts Amherst, 686 N. Pleasant St., 149A Goessmann, Amherst, MA 01003, USADepartment of Environmental Health Sciences, University of Massachusetts Amherst, 686 N. Pleasant St., 149A Goessmann, Amherst, MA 01003, USAFor the majority of lipophilic compounds, adipose tissue is traditionally considered as a storage depot and only rarely as a target organ. Meanwhile, abnormalities in adipose tissue physiology induced by chemical exposure may contribute to the current epidemic of obesity and metabolic diseases. Polybrominated diphenyl ethers (PBDEs) are a group of lipophilic flame retardants found in the majority of human samples in North America. Their ability to alter the physiology of adipose tissue is unknown. We exposed pregnant mice to 0.2 mg/kg body weight/day of BDE-47 perinatally. Transcriptomic changes in gonadal adipose tissue were analyzed in male offspring using the RNA-seq approach with subsequent bioinformatic analysis. The expression of genes of coagulation and complement cascade, de novo lipogenesis, and xenobiotic metabolism was altered in response to BDE-47 exposure. The affected molecular network included the following hubs: PPARα, HNF1A, and HNF4. These findings suggest that adipose tissue should be considered a target tissue for BDE-47, in addition to its role as a storage depot. This study also builds a background for a targeted search of sensitive phenotypic endpoints of BDE-47 exposure, including lipid profile parameters and coagulation factors in circulation. Additional studies are needed to investigate the role of PBDEs as an obesogen.http://www.mdpi.com/2305-6304/6/2/21polybrominated diphenyl etherPBDEBDE-47adiposetranscriptomicgenomicobesogencomplement and coagulation cascadede novo lipogenesismetabolism |
| spellingShingle | Aser Abrha Alexander Suvorov Transcriptomic Analysis of Gonadal Adipose Tissue in Male Mice Exposed Perinatally to 2,2′,4,4′-Tetrabromodiphenyl Ether (BDE-47) Toxics polybrominated diphenyl ether PBDE BDE-47 adipose transcriptomic genomic obesogen complement and coagulation cascade de novo lipogenesis metabolism |
| title | Transcriptomic Analysis of Gonadal Adipose Tissue in Male Mice Exposed Perinatally to 2,2′,4,4′-Tetrabromodiphenyl Ether (BDE-47) |
| title_full | Transcriptomic Analysis of Gonadal Adipose Tissue in Male Mice Exposed Perinatally to 2,2′,4,4′-Tetrabromodiphenyl Ether (BDE-47) |
| title_fullStr | Transcriptomic Analysis of Gonadal Adipose Tissue in Male Mice Exposed Perinatally to 2,2′,4,4′-Tetrabromodiphenyl Ether (BDE-47) |
| title_full_unstemmed | Transcriptomic Analysis of Gonadal Adipose Tissue in Male Mice Exposed Perinatally to 2,2′,4,4′-Tetrabromodiphenyl Ether (BDE-47) |
| title_short | Transcriptomic Analysis of Gonadal Adipose Tissue in Male Mice Exposed Perinatally to 2,2′,4,4′-Tetrabromodiphenyl Ether (BDE-47) |
| title_sort | transcriptomic analysis of gonadal adipose tissue in male mice exposed perinatally to 2 2 4 4 tetrabromodiphenyl ether bde 47 |
| topic | polybrominated diphenyl ether PBDE BDE-47 adipose transcriptomic genomic obesogen complement and coagulation cascade de novo lipogenesis metabolism |
| url | http://www.mdpi.com/2305-6304/6/2/21 |
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