Antibiotic Susceptibility and Clarithromycin Resistance Determinants in <i>Helicobacter pylori</i> in the Northeast of Spain: A One-Year Prospective Study
<i>Helicobacter pylori</i> is one of the most widespread infections, and it is reaching alarming resistance levels worldwide. The recommended first-line empirical treatment differs according to the local rate of clarithromycin resistance. Macrolide resistance is mainly associated with th...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/2/356 |
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| author | Saray Mormeneo Bayo Alba Bellés Bellés Diego Vázquez Gómez Montserrat Planella de Rubinat Diana Carolina Bayas Pastor Arturo Morales Portillo Alfredo Jover Sáenz Éric López González Núria Prim Mercè García-González |
| author_facet | Saray Mormeneo Bayo Alba Bellés Bellés Diego Vázquez Gómez Montserrat Planella de Rubinat Diana Carolina Bayas Pastor Arturo Morales Portillo Alfredo Jover Sáenz Éric López González Núria Prim Mercè García-González |
| author_sort | Saray Mormeneo Bayo |
| collection | DOAJ |
| description | <i>Helicobacter pylori</i> is one of the most widespread infections, and it is reaching alarming resistance levels worldwide. The recommended first-line empirical treatment differs according to the local rate of clarithromycin resistance. Macrolide resistance is mainly associated with three point mutations in the 23S rRNA gene. The aim of this study was to describe the antibiotic susceptibility of <i>H. pylori</i> in our healthcare area and the main mechanisms involved in clarithromycin resistance. Gastric biopsies (<i>n</i> = 641) were collected and cultured in a one-year prospective study. Antibiotic susceptibility testing was performed by gradient diffusion. A multiplex real-time PCR test (Allplex<sup>TM</sup><i>H.pylori</i> & ClariR Assay, Seegene) was used to detect the most frequent mutations associated with clarithromycin resistance. Overall, 141 isolates were available for antibiotic susceptibility testing. The highest resistance rates were detected in metronidazole and levofloxacin. The rate of clarithromycin resistance was 12.1%, and the associated mutations were A2143G and A2142G. More than half of the clarithromycin-resistant isolates presented high MIC values (>256 mg/L). Tetracycline resistance was not detected, suggesting that therapies that contain tetracycline could be a suitable option. The low clarithromycin resistance rate coupled with the high rates of metronidazole resistance may support the recovery of the classical triple therapy in our healthcare area. |
| first_indexed | 2024-03-11T09:14:42Z |
| format | Article |
| id | doaj.art-13b166aef30045cfa9f9d5ddf3ea3394 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-11T09:14:42Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-13b166aef30045cfa9f9d5ddf3ea33942023-11-16T18:43:52ZengMDPI AGAntibiotics2079-63822023-02-0112235610.3390/antibiotics12020356Antibiotic Susceptibility and Clarithromycin Resistance Determinants in <i>Helicobacter pylori</i> in the Northeast of Spain: A One-Year Prospective StudySaray Mormeneo Bayo0Alba Bellés Bellés1Diego Vázquez Gómez2Montserrat Planella de Rubinat3Diana Carolina Bayas Pastor4Arturo Morales Portillo5Alfredo Jover Sáenz6Éric López González7Núria Prim8Mercè García-González9Unidad de Microbiología, Hospital Universitari Arnau de Vilanova de Lleida, Institut Català de la Salut, 25198 Lleida, SpainUnidad de Microbiología, Hospital Universitari Arnau de Vilanova de Lleida, Institut Català de la Salut, 25198 Lleida, SpainInstitut de Recerca Biomèdica de Lleida Fundació Dr. Pifarré, IRBLleida, 25198 Lleida, SpainInstitut de Recerca Biomèdica de Lleida Fundació Dr. Pifarré, IRBLleida, 25198 Lleida, SpainInstitut de Recerca Biomèdica de Lleida Fundació Dr. Pifarré, IRBLleida, 25198 Lleida, SpainServicio de Farmacia, Hospital Universitari Arnau de Vilanova de Lleida, 25198 Lleida, SpainInstitut de Recerca Biomèdica de Lleida Fundació Dr. Pifarré, IRBLleida, 25198 Lleida, SpainUnidad de Microbiología, Hospital Universitari Arnau de Vilanova de Lleida, Institut Català de la Salut, 25198 Lleida, SpainUnidad de Microbiología, Hospital Universitari Arnau de Vilanova de Lleida, Institut Català de la Salut, 25198 Lleida, SpainUnidad de Microbiología, Hospital Universitari Arnau de Vilanova de Lleida, Institut Català de la Salut, 25198 Lleida, Spain<i>Helicobacter pylori</i> is one of the most widespread infections, and it is reaching alarming resistance levels worldwide. The recommended first-line empirical treatment differs according to the local rate of clarithromycin resistance. Macrolide resistance is mainly associated with three point mutations in the 23S rRNA gene. The aim of this study was to describe the antibiotic susceptibility of <i>H. pylori</i> in our healthcare area and the main mechanisms involved in clarithromycin resistance. Gastric biopsies (<i>n</i> = 641) were collected and cultured in a one-year prospective study. Antibiotic susceptibility testing was performed by gradient diffusion. A multiplex real-time PCR test (Allplex<sup>TM</sup><i>H.pylori</i> & ClariR Assay, Seegene) was used to detect the most frequent mutations associated with clarithromycin resistance. Overall, 141 isolates were available for antibiotic susceptibility testing. The highest resistance rates were detected in metronidazole and levofloxacin. The rate of clarithromycin resistance was 12.1%, and the associated mutations were A2143G and A2142G. More than half of the clarithromycin-resistant isolates presented high MIC values (>256 mg/L). Tetracycline resistance was not detected, suggesting that therapies that contain tetracycline could be a suitable option. The low clarithromycin resistance rate coupled with the high rates of metronidazole resistance may support the recovery of the classical triple therapy in our healthcare area.https://www.mdpi.com/2079-6382/12/2/356<i>Helicobacter pylori</i>clarithromycin resistancetriple therapy |
| spellingShingle | Saray Mormeneo Bayo Alba Bellés Bellés Diego Vázquez Gómez Montserrat Planella de Rubinat Diana Carolina Bayas Pastor Arturo Morales Portillo Alfredo Jover Sáenz Éric López González Núria Prim Mercè García-González Antibiotic Susceptibility and Clarithromycin Resistance Determinants in <i>Helicobacter pylori</i> in the Northeast of Spain: A One-Year Prospective Study Antibiotics <i>Helicobacter pylori</i> clarithromycin resistance triple therapy |
| title | Antibiotic Susceptibility and Clarithromycin Resistance Determinants in <i>Helicobacter pylori</i> in the Northeast of Spain: A One-Year Prospective Study |
| title_full | Antibiotic Susceptibility and Clarithromycin Resistance Determinants in <i>Helicobacter pylori</i> in the Northeast of Spain: A One-Year Prospective Study |
| title_fullStr | Antibiotic Susceptibility and Clarithromycin Resistance Determinants in <i>Helicobacter pylori</i> in the Northeast of Spain: A One-Year Prospective Study |
| title_full_unstemmed | Antibiotic Susceptibility and Clarithromycin Resistance Determinants in <i>Helicobacter pylori</i> in the Northeast of Spain: A One-Year Prospective Study |
| title_short | Antibiotic Susceptibility and Clarithromycin Resistance Determinants in <i>Helicobacter pylori</i> in the Northeast of Spain: A One-Year Prospective Study |
| title_sort | antibiotic susceptibility and clarithromycin resistance determinants in i helicobacter pylori i in the northeast of spain a one year prospective study |
| topic | <i>Helicobacter pylori</i> clarithromycin resistance triple therapy |
| url | https://www.mdpi.com/2079-6382/12/2/356 |
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