Generation of a human embryonic stem cell line, NERCe003-A-1, with lentivirus vector-mediated inducible CTNNB1 overexpression
The human embryonic stem cell (hESC) line NERCe003-A-1 was generated by introducing lentiviral-vector–mediated tetracycline-inducible β-catenin expression into a normal hESC line, NERCe003-A. The resulting cell line can overexpress the β-catenin protein, encoded by the CTNNB1 gene, after exposure to...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-04-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506118300266 |
| _version_ | 1830462155012440064 |
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| author | Yang Wang Juan Yu Lvjun Liu Wen Li Xingxiang Duan Yingying Peng Sicong Zeng Qi Ouyang Guangxiu Lu Ge Lin Yi Sun |
| author_facet | Yang Wang Juan Yu Lvjun Liu Wen Li Xingxiang Duan Yingying Peng Sicong Zeng Qi Ouyang Guangxiu Lu Ge Lin Yi Sun |
| author_sort | Yang Wang |
| collection | DOAJ |
| description | The human embryonic stem cell (hESC) line NERCe003-A-1 was generated by introducing lentiviral-vector–mediated tetracycline-inducible β-catenin expression into a normal hESC line, NERCe003-A. The resulting cell line can overexpress the β-catenin protein, encoded by the CTNNB1 gene, after exposure to doxycycline (Dox). CTNNB1 gene expression was confirmed by quantitative PCR (qPCR) and immunofluorescence assays. Further characterization confirmed that the NERCe003-A-1 cell line expresses typical pluripotency markers and has the ability to form the three germ layers both in vitro and in vivo. |
| first_indexed | 2024-12-21T11:40:53Z |
| format | Article |
| id | doaj.art-13b1f817c91c43d38dfbeaaf8c67d27f |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-21T11:40:53Z |
| publishDate | 2018-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-13b1f817c91c43d38dfbeaaf8c67d27f2022-12-21T19:05:18ZengElsevierStem Cell Research1873-50612018-04-01286165Generation of a human embryonic stem cell line, NERCe003-A-1, with lentivirus vector-mediated inducible CTNNB1 overexpressionYang Wang0Juan Yu1Lvjun Liu2Wen Li3Xingxiang Duan4Yingying Peng5Sicong Zeng6Qi Ouyang7Guangxiu Lu8Ge Lin9Yi Sun10Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, ChinaNational Engineering and Research Center of Human Stem Cells, Changsha, ChinaInstitute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, ChinaInstitute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Reproductive & Genetic Hospital of CITIC-Xiangya, Changsha, ChinaInstitute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, ChinaInstitute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, ChinaInstitute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cells, Changsha, ChinaInstitute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cells, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, ChinaNational Engineering and Research Center of Human Stem Cells, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Reproductive & Genetic Hospital of CITIC-Xiangya, Changsha, ChinaInstitute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cells, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Reproductive & Genetic Hospital of CITIC-Xiangya, Changsha, ChinaInstitute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cells, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Corresponding author at: Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.The human embryonic stem cell (hESC) line NERCe003-A-1 was generated by introducing lentiviral-vector–mediated tetracycline-inducible β-catenin expression into a normal hESC line, NERCe003-A. The resulting cell line can overexpress the β-catenin protein, encoded by the CTNNB1 gene, after exposure to doxycycline (Dox). CTNNB1 gene expression was confirmed by quantitative PCR (qPCR) and immunofluorescence assays. Further characterization confirmed that the NERCe003-A-1 cell line expresses typical pluripotency markers and has the ability to form the three germ layers both in vitro and in vivo.http://www.sciencedirect.com/science/article/pii/S1873506118300266 |
| spellingShingle | Yang Wang Juan Yu Lvjun Liu Wen Li Xingxiang Duan Yingying Peng Sicong Zeng Qi Ouyang Guangxiu Lu Ge Lin Yi Sun Generation of a human embryonic stem cell line, NERCe003-A-1, with lentivirus vector-mediated inducible CTNNB1 overexpression Stem Cell Research |
| title | Generation of a human embryonic stem cell line, NERCe003-A-1, with lentivirus vector-mediated inducible CTNNB1 overexpression |
| title_full | Generation of a human embryonic stem cell line, NERCe003-A-1, with lentivirus vector-mediated inducible CTNNB1 overexpression |
| title_fullStr | Generation of a human embryonic stem cell line, NERCe003-A-1, with lentivirus vector-mediated inducible CTNNB1 overexpression |
| title_full_unstemmed | Generation of a human embryonic stem cell line, NERCe003-A-1, with lentivirus vector-mediated inducible CTNNB1 overexpression |
| title_short | Generation of a human embryonic stem cell line, NERCe003-A-1, with lentivirus vector-mediated inducible CTNNB1 overexpression |
| title_sort | generation of a human embryonic stem cell line nerce003 a 1 with lentivirus vector mediated inducible ctnnb1 overexpression |
| url | http://www.sciencedirect.com/science/article/pii/S1873506118300266 |
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