Nanobubble Mediated Gene Delivery in Conjunction With a Hand-Held Ultrasound Scanner
Recent research has revealed that nanobubbles (NBs) can be an effective tool for gene transfection in conjunction with therapeutic ultrasound (US). However, an approach to apply commercially available hand-held diagnostic US scanners for this purpose has not been evaluated as of now. In the present...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-04-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphar.2020.00363/full |
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| author | Hiroshi Kida Koyo Nishimura Koki Ogawa Akiko Watanabe Loreto B. Feril Yutaka Irie Hitomi Endo Shigeru Kawakami Katsuro Tachibana |
| author_facet | Hiroshi Kida Koyo Nishimura Koki Ogawa Akiko Watanabe Loreto B. Feril Yutaka Irie Hitomi Endo Shigeru Kawakami Katsuro Tachibana |
| author_sort | Hiroshi Kida |
| collection | DOAJ |
| description | Recent research has revealed that nanobubbles (NBs) can be an effective tool for gene transfection in conjunction with therapeutic ultrasound (US). However, an approach to apply commercially available hand-held diagnostic US scanners for this purpose has not been evaluated as of now. In the present study, we first compared in vitro, the efficiency of gene transfer (pCMV-Luciferase) with lipid-based and albumin-based NBs irradiated by therapeutic US (1MHz, 5.0 W/cm2) in oral squamous carcinoma cell line HSC-2. Secondly, we similarly examined if gene transfer in mice is possible using a clinical hand-held US scanner (2.3MHz, MI 1.0). Results showed that lipid-based NBs induced more gene transfection compared to albumin-based NBs, in vitro. Furthermore, significant gene transfer was also obtained in mice liver with lipid-based NBs. Sub-micro sized bubbles proved to be a powerful gene transfer reagent in combination with conventional hand-held ultrasonic diagnostic device. |
| first_indexed | 2024-12-20T01:52:13Z |
| format | Article |
| id | doaj.art-13b370540fff42c38c1171a97d6af510 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-20T01:52:13Z |
| publishDate | 2020-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-13b370540fff42c38c1171a97d6af5102022-12-21T19:57:36ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122020-04-011110.3389/fphar.2020.00363512794Nanobubble Mediated Gene Delivery in Conjunction With a Hand-Held Ultrasound ScannerHiroshi Kida0Koyo Nishimura1Koki Ogawa2Akiko Watanabe3Loreto B. Feril4Yutaka Irie5Hitomi Endo6Shigeru Kawakami7Katsuro Tachibana8Department of Anatomy, Fukuoka University School of Medicine, Fukuoka, JapanDepartment of Pharmaceutical Informatics, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, JapanDepartment of Pharmaceutical Informatics, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, JapanDepartment of Anatomy, Fukuoka University School of Medicine, Fukuoka, JapanDepartment of Anatomy, Fukuoka University School of Medicine, Fukuoka, JapanDepartment of Anatomy, Fukuoka University School of Medicine, Fukuoka, JapanDepartment of Anatomy, Fukuoka University School of Medicine, Fukuoka, JapanDepartment of Pharmaceutical Informatics, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, JapanDepartment of Anatomy, Fukuoka University School of Medicine, Fukuoka, JapanRecent research has revealed that nanobubbles (NBs) can be an effective tool for gene transfection in conjunction with therapeutic ultrasound (US). However, an approach to apply commercially available hand-held diagnostic US scanners for this purpose has not been evaluated as of now. In the present study, we first compared in vitro, the efficiency of gene transfer (pCMV-Luciferase) with lipid-based and albumin-based NBs irradiated by therapeutic US (1MHz, 5.0 W/cm2) in oral squamous carcinoma cell line HSC-2. Secondly, we similarly examined if gene transfer in mice is possible using a clinical hand-held US scanner (2.3MHz, MI 1.0). Results showed that lipid-based NBs induced more gene transfection compared to albumin-based NBs, in vitro. Furthermore, significant gene transfer was also obtained in mice liver with lipid-based NBs. Sub-micro sized bubbles proved to be a powerful gene transfer reagent in combination with conventional hand-held ultrasonic diagnostic device.https://www.frontiersin.org/article/10.3389/fphar.2020.00363/fullgene transfectionsonoporationultrasoundnanobubbleultrasound diagnostic device |
| spellingShingle | Hiroshi Kida Koyo Nishimura Koki Ogawa Akiko Watanabe Loreto B. Feril Yutaka Irie Hitomi Endo Shigeru Kawakami Katsuro Tachibana Nanobubble Mediated Gene Delivery in Conjunction With a Hand-Held Ultrasound Scanner Frontiers in Pharmacology gene transfection sonoporation ultrasound nanobubble ultrasound diagnostic device |
| title | Nanobubble Mediated Gene Delivery in Conjunction With a Hand-Held Ultrasound Scanner |
| title_full | Nanobubble Mediated Gene Delivery in Conjunction With a Hand-Held Ultrasound Scanner |
| title_fullStr | Nanobubble Mediated Gene Delivery in Conjunction With a Hand-Held Ultrasound Scanner |
| title_full_unstemmed | Nanobubble Mediated Gene Delivery in Conjunction With a Hand-Held Ultrasound Scanner |
| title_short | Nanobubble Mediated Gene Delivery in Conjunction With a Hand-Held Ultrasound Scanner |
| title_sort | nanobubble mediated gene delivery in conjunction with a hand held ultrasound scanner |
| topic | gene transfection sonoporation ultrasound nanobubble ultrasound diagnostic device |
| url | https://www.frontiersin.org/article/10.3389/fphar.2020.00363/full |
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