Poly[oligo(ethylene glycol) methacrylate]-<i>b</i>-poly[(vinyl benzyl trimethylammonium chloride)] Based Multifunctional Hybrid Nanostructures Encapsulating Magnetic Nanoparticles and DNA
We report on the preparation of novel and multifunctional hybrid spherical-shaped nanostructures involving a double-hydrophilic block copolymer, namely the neutral cationic poly[oligo(ethylene glycol) methacrylate]-<i>b</i>-poly[(vinyl benzyl trimethylammonium chloride)] (POEGMA-<i>...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-06-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/12/6/1283 |
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| author | Angeliki Chroni Aleksander Forys Barbara Trzebicka Adam Alemayehu Vaclav Tyrpekl Stergios Pispas |
| author_facet | Angeliki Chroni Aleksander Forys Barbara Trzebicka Adam Alemayehu Vaclav Tyrpekl Stergios Pispas |
| author_sort | Angeliki Chroni |
| collection | DOAJ |
| description | We report on the preparation of novel and multifunctional hybrid spherical-shaped nanostructures involving a double-hydrophilic block copolymer, namely the neutral cationic poly[oligo(ethylene glycol) methacrylate]-<i>b</i>-poly[(vinyl benzyl trimethylammonium chloride)] (POEGMA-<i>b</i>-PVBTMAC) diblock copolymer, initially complexed with hydrophilic anionic magnetic nanoparticles (MNPs), and subsequently, with short deoxyribonucleic acid (113 bases DNA). The POEGMA-<i>b</i>-PVBTMAC copolymer, the copolymer/MNPs and the copolymer/MNPs/DNA tricomponent hybrid electrostatic complexes were studied by dynamic/electrophoretic light scattering (DLS/ELS) and cryogenic transmission electron microscopy (cryo-TEM) techniques for the determination of their structure and solution properties. The MNPs were complexed efficiently with the oppositely charged diblock chains, leading to well-defined hybrid organic–inorganic spherical-shaped nanostructures. A significant aggregation tendency of the MNPs is noticed in cryo-TEM measurements after the electrostatic complexation of DNA, implying an accumulation of the DNA macromolecules on the surface of the hybrid tricomponent complexes. Magnetophoretic experiments verified that the MNPs maintain their magnetic properties after the complexation initially with the copolymer, and subsequently, within the block polyelectrolyte/MNPs/DNA nanostructures. |
| first_indexed | 2024-03-10T19:23:28Z |
| format | Article |
| id | doaj.art-13b3f55a8c104c18af9e6e6ffb725992 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T19:23:28Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-13b3f55a8c104c18af9e6e6ffb7259922023-11-20T02:46:59ZengMDPI AGPolymers2073-43602020-06-01126128310.3390/polym12061283Poly[oligo(ethylene glycol) methacrylate]-<i>b</i>-poly[(vinyl benzyl trimethylammonium chloride)] Based Multifunctional Hybrid Nanostructures Encapsulating Magnetic Nanoparticles and DNAAngeliki Chroni0Aleksander Forys1Barbara Trzebicka2Adam Alemayehu3Vaclav Tyrpekl4Stergios Pispas5Theoretical and Physical Chemistry Institute, National Hellenic Research Foundation, 48 Vassileos Constantinou Avenue, 11635 Athens, GreeceCentre of Polymer and Carbon Materials, Polish Academy of Sciences, 34 ul. M. Curie-Skłodowskiej, 41-819 Zabrze, PolandCentre of Polymer and Carbon Materials, Polish Academy of Sciences, 34 ul. M. Curie-Skłodowskiej, 41-819 Zabrze, PolandDepartment of Inorganic Chemistry, Faculty of Science, Charles University in Prague, Albertov 6, 128-43 Praha 2, 11636 Prague, Czech RepublicDepartment of Inorganic Chemistry, Faculty of Science, Charles University in Prague, Albertov 6, 128-43 Praha 2, 11636 Prague, Czech RepublicTheoretical and Physical Chemistry Institute, National Hellenic Research Foundation, 48 Vassileos Constantinou Avenue, 11635 Athens, GreeceWe report on the preparation of novel and multifunctional hybrid spherical-shaped nanostructures involving a double-hydrophilic block copolymer, namely the neutral cationic poly[oligo(ethylene glycol) methacrylate]-<i>b</i>-poly[(vinyl benzyl trimethylammonium chloride)] (POEGMA-<i>b</i>-PVBTMAC) diblock copolymer, initially complexed with hydrophilic anionic magnetic nanoparticles (MNPs), and subsequently, with short deoxyribonucleic acid (113 bases DNA). The POEGMA-<i>b</i>-PVBTMAC copolymer, the copolymer/MNPs and the copolymer/MNPs/DNA tricomponent hybrid electrostatic complexes were studied by dynamic/electrophoretic light scattering (DLS/ELS) and cryogenic transmission electron microscopy (cryo-TEM) techniques for the determination of their structure and solution properties. The MNPs were complexed efficiently with the oppositely charged diblock chains, leading to well-defined hybrid organic–inorganic spherical-shaped nanostructures. A significant aggregation tendency of the MNPs is noticed in cryo-TEM measurements after the electrostatic complexation of DNA, implying an accumulation of the DNA macromolecules on the surface of the hybrid tricomponent complexes. Magnetophoretic experiments verified that the MNPs maintain their magnetic properties after the complexation initially with the copolymer, and subsequently, within the block polyelectrolyte/MNPs/DNA nanostructures.https://www.mdpi.com/2073-4360/12/6/1283double-hydrophilic block copolymerstheranosticscationic polyelectrolytesmagnetic nanoparticlesgene deliveryhybrid nanostructures |
| spellingShingle | Angeliki Chroni Aleksander Forys Barbara Trzebicka Adam Alemayehu Vaclav Tyrpekl Stergios Pispas Poly[oligo(ethylene glycol) methacrylate]-<i>b</i>-poly[(vinyl benzyl trimethylammonium chloride)] Based Multifunctional Hybrid Nanostructures Encapsulating Magnetic Nanoparticles and DNA Polymers double-hydrophilic block copolymers theranostics cationic polyelectrolytes magnetic nanoparticles gene delivery hybrid nanostructures |
| title | Poly[oligo(ethylene glycol) methacrylate]-<i>b</i>-poly[(vinyl benzyl trimethylammonium chloride)] Based Multifunctional Hybrid Nanostructures Encapsulating Magnetic Nanoparticles and DNA |
| title_full | Poly[oligo(ethylene glycol) methacrylate]-<i>b</i>-poly[(vinyl benzyl trimethylammonium chloride)] Based Multifunctional Hybrid Nanostructures Encapsulating Magnetic Nanoparticles and DNA |
| title_fullStr | Poly[oligo(ethylene glycol) methacrylate]-<i>b</i>-poly[(vinyl benzyl trimethylammonium chloride)] Based Multifunctional Hybrid Nanostructures Encapsulating Magnetic Nanoparticles and DNA |
| title_full_unstemmed | Poly[oligo(ethylene glycol) methacrylate]-<i>b</i>-poly[(vinyl benzyl trimethylammonium chloride)] Based Multifunctional Hybrid Nanostructures Encapsulating Magnetic Nanoparticles and DNA |
| title_short | Poly[oligo(ethylene glycol) methacrylate]-<i>b</i>-poly[(vinyl benzyl trimethylammonium chloride)] Based Multifunctional Hybrid Nanostructures Encapsulating Magnetic Nanoparticles and DNA |
| title_sort | poly oligo ethylene glycol methacrylate i b i poly vinyl benzyl trimethylammonium chloride based multifunctional hybrid nanostructures encapsulating magnetic nanoparticles and dna |
| topic | double-hydrophilic block copolymers theranostics cationic polyelectrolytes magnetic nanoparticles gene delivery hybrid nanostructures |
| url | https://www.mdpi.com/2073-4360/12/6/1283 |
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