Evidence of Bi-Directional Volatile-Mediated Communication between Drought-Stressed and Well-Watered Grapevines (<i>Vitis vinifera</i> L.)
The volatile-mediated interplay between stressed and non-stressed plants has been described in many studies involving both biotic and abiotic stresses as a one-way channel. However, very little is known about the molecular basis and mechanisms by which volatile organic compounds (VOCs) mediate plant...
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| Format: | Article |
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MDPI AG
2023-06-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/7/1747 |
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| author | Joanah Midzi David W. Jeffery Ute Baumann Dimitra L. Capone Suzy Y. Rogiers Vinay Pagay |
| author_facet | Joanah Midzi David W. Jeffery Ute Baumann Dimitra L. Capone Suzy Y. Rogiers Vinay Pagay |
| author_sort | Joanah Midzi |
| collection | DOAJ |
| description | The volatile-mediated interplay between stressed and non-stressed plants has been described in many studies involving both biotic and abiotic stresses as a one-way channel. However, very little is known about the molecular basis and mechanisms by which volatile organic compounds (VOCs) mediate plant communication between drought-stressed ‘emitter’ plants and non-stressed ‘receiver’ neighbours for the defence against impending stress challenges. Aiming to address this in grapevine, this study investigated the effect of two-way VOC exchange between stressed and non-stressed <i>Vitis vinifera</i> L. cv. Shiraz during drought and recovery using four treatments: isolated well-watered (WW) vines, isolated drought-stressed (DS) vines, and co-located DS ‘emitter’ and WW ‘receiver’ vines in a growth room. The results obtained from solid-phase microextraction (SPME) gas chromatography mass spectrometry (GC-MS) analysis showed a synchronised decline in α-pinene concentration in the co-located treatment vines and higher isoprene levels in the DS emitters compared to the isolated DS vines. Targeted gene expression analysis further identified the over-expression of a key gene, allene oxide synthase (AOS), in the jasmonic acid (JA) biosynthesis pathway during peak drought in the DS emitter. Transcript expression of chorismate synthase (CHORS) and α-pinene synthase (VvPNaPin1) showed similar trends in the DS emitter. The results suggest that isoprene and α-pinene may be interplant signalling molecules used by grapevine during drought. To the best of our knowledge, this is the first report of a bi-directional interaction in grapevine between the emitters and receivers under drought stress mediated by the JA and terpenoid biosynthesis pathways. |
| first_indexed | 2024-03-11T01:23:12Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-11T01:23:12Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-13b5bb55a95f4c1083f48e796474d6d02023-11-18T17:55:36ZengMDPI AGAgronomy2073-43952023-06-01137174710.3390/agronomy13071747Evidence of Bi-Directional Volatile-Mediated Communication between Drought-Stressed and Well-Watered Grapevines (<i>Vitis vinifera</i> L.)Joanah Midzi0David W. Jeffery1Ute Baumann2Dimitra L. Capone3Suzy Y. Rogiers4Vinay Pagay5School of Agriculture, Food and Wine, The University of Adelaide, Glen Osmond, SA 5064, AustraliaSchool of Agriculture, Food and Wine, The University of Adelaide, Glen Osmond, SA 5064, AustraliaSchool of Agriculture, Food and Wine, The University of Adelaide, Glen Osmond, SA 5064, AustraliaSchool of Agriculture, Food and Wine, The University of Adelaide, Glen Osmond, SA 5064, AustraliaAustralian Research Council Training Centre for Innovative Wine Production, Glen Osmond, SA 5064, AustraliaSchool of Agriculture, Food and Wine, The University of Adelaide, Glen Osmond, SA 5064, AustraliaThe volatile-mediated interplay between stressed and non-stressed plants has been described in many studies involving both biotic and abiotic stresses as a one-way channel. However, very little is known about the molecular basis and mechanisms by which volatile organic compounds (VOCs) mediate plant communication between drought-stressed ‘emitter’ plants and non-stressed ‘receiver’ neighbours for the defence against impending stress challenges. Aiming to address this in grapevine, this study investigated the effect of two-way VOC exchange between stressed and non-stressed <i>Vitis vinifera</i> L. cv. Shiraz during drought and recovery using four treatments: isolated well-watered (WW) vines, isolated drought-stressed (DS) vines, and co-located DS ‘emitter’ and WW ‘receiver’ vines in a growth room. The results obtained from solid-phase microextraction (SPME) gas chromatography mass spectrometry (GC-MS) analysis showed a synchronised decline in α-pinene concentration in the co-located treatment vines and higher isoprene levels in the DS emitters compared to the isolated DS vines. Targeted gene expression analysis further identified the over-expression of a key gene, allene oxide synthase (AOS), in the jasmonic acid (JA) biosynthesis pathway during peak drought in the DS emitter. Transcript expression of chorismate synthase (CHORS) and α-pinene synthase (VvPNaPin1) showed similar trends in the DS emitter. The results suggest that isoprene and α-pinene may be interplant signalling molecules used by grapevine during drought. To the best of our knowledge, this is the first report of a bi-directional interaction in grapevine between the emitters and receivers under drought stress mediated by the JA and terpenoid biosynthesis pathways.https://www.mdpi.com/2073-4395/13/7/1747α-pineneisopreneplant–plant interactionsVOC primingVOCsabiotic stress |
| spellingShingle | Joanah Midzi David W. Jeffery Ute Baumann Dimitra L. Capone Suzy Y. Rogiers Vinay Pagay Evidence of Bi-Directional Volatile-Mediated Communication between Drought-Stressed and Well-Watered Grapevines (<i>Vitis vinifera</i> L.) Agronomy α-pinene isoprene plant–plant interactions VOC priming VOCs abiotic stress |
| title | Evidence of Bi-Directional Volatile-Mediated Communication between Drought-Stressed and Well-Watered Grapevines (<i>Vitis vinifera</i> L.) |
| title_full | Evidence of Bi-Directional Volatile-Mediated Communication between Drought-Stressed and Well-Watered Grapevines (<i>Vitis vinifera</i> L.) |
| title_fullStr | Evidence of Bi-Directional Volatile-Mediated Communication between Drought-Stressed and Well-Watered Grapevines (<i>Vitis vinifera</i> L.) |
| title_full_unstemmed | Evidence of Bi-Directional Volatile-Mediated Communication between Drought-Stressed and Well-Watered Grapevines (<i>Vitis vinifera</i> L.) |
| title_short | Evidence of Bi-Directional Volatile-Mediated Communication between Drought-Stressed and Well-Watered Grapevines (<i>Vitis vinifera</i> L.) |
| title_sort | evidence of bi directional volatile mediated communication between drought stressed and well watered grapevines i vitis vinifera i l |
| topic | α-pinene isoprene plant–plant interactions VOC priming VOCs abiotic stress |
| url | https://www.mdpi.com/2073-4395/13/7/1747 |
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