The Effects of <i>Lycium chinense</i>, <i>Cuscuta chinensis</i>, <i>Senna tora</i>, <i>Ophiopogon japonicus</i>, and <i>Dendrobium nobile</i> Decoction on a Dry Eye Mouse Model
<i>Background and objective</i>: Dry eye disease (DED) is a relatively common disorder associated with abnormal tear film and the ocular surface that causes ocular irritation, dryness, visual impairment, and damage to the cornea. DED is not a life-threatening disease but causes discomfor...
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MDPI AG
2022-08-01
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| Series: | Medicina |
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| Online Access: | https://www.mdpi.com/1648-9144/58/8/1134 |
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| author | Cheng-Chan Yang Jia-Ying Chien Yu-Yau Chou Jhih-Wei Ciou Shun-Ping Huang |
| author_facet | Cheng-Chan Yang Jia-Ying Chien Yu-Yau Chou Jhih-Wei Ciou Shun-Ping Huang |
| author_sort | Cheng-Chan Yang |
| collection | DOAJ |
| description | <i>Background and objective</i>: Dry eye disease (DED) is a relatively common disorder associated with abnormal tear film and the ocular surface that causes ocular irritation, dryness, visual impairment, and damage to the cornea. DED is not a life-threatening disease but causes discomfort and multifactorial disorders in vision that affect daily life. It has been reported that all traditional medicinal plants exhibit anti-inflammatory effects on several diseases. We hypothesized that the decoction ameliorated ocular irritation and decreased cytokine expression in the cornea. This study aimed to investigate the molecular mechanisms of DED and discover a therapeutic strategy to reduce corneal inflammation. <i>Material and Methods</i>: We used a DED mouse model with extraorbital lacrimal gland (ELG) excision and treated the mice with a decoction of five traditional medicines: <i>Lycium chinense</i>, <i>Cuscuta chinensis</i>, <i>Senna tora</i>, <i>Ophiopogon japonicus</i>, and <i>Dendrobium nobile</i> for 3 months. The tear osmolarity and the ocular surface staining were evaluated as indicators of DED. Immunohistochemistry was used to detect the level of inflammation on the cornea. <i>Results</i>: After treatment with the decoction for three months, epithelial erosions and desquamation were reduced, the intact of corneal endothelium was maintained, and tear osmolarity was restored in the eyes. The IL-1β-associated inflammatory response was reduced in the cornea in the DED model. <i>Conclusions:</i> These data suggested that a mixture of traditional medicines might be a novel therapy to treat DED. |
| first_indexed | 2024-03-09T04:06:14Z |
| format | Article |
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| issn | 1010-660X 1648-9144 |
| language | English |
| last_indexed | 2024-03-09T04:06:14Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Medicina |
| spelling | doaj.art-13b7269d68424c099a22c34abfc14d882023-12-03T14:05:13ZengMDPI AGMedicina1010-660X1648-91442022-08-01588113410.3390/medicina58081134The Effects of <i>Lycium chinense</i>, <i>Cuscuta chinensis</i>, <i>Senna tora</i>, <i>Ophiopogon japonicus</i>, and <i>Dendrobium nobile</i> Decoction on a Dry Eye Mouse ModelCheng-Chan Yang0Jia-Ying Chien1Yu-Yau Chou2Jhih-Wei Ciou3Shun-Ping Huang4Department of Chinese Medicine, Hualien Tzu Chi Hospital, Hualien 970, TaiwanInstitute of Medical Science, Tzu Chi University, Hualien 970, TaiwanDepartment of Molecular Biology and Human Genetics, Tzu Chi University, Hualien 970, TaiwanDepartment of Molecular Biology and Human Genetics, Tzu Chi University, Hualien 970, TaiwanInstitute of Medical Science, Tzu Chi University, Hualien 970, Taiwan<i>Background and objective</i>: Dry eye disease (DED) is a relatively common disorder associated with abnormal tear film and the ocular surface that causes ocular irritation, dryness, visual impairment, and damage to the cornea. DED is not a life-threatening disease but causes discomfort and multifactorial disorders in vision that affect daily life. It has been reported that all traditional medicinal plants exhibit anti-inflammatory effects on several diseases. We hypothesized that the decoction ameliorated ocular irritation and decreased cytokine expression in the cornea. This study aimed to investigate the molecular mechanisms of DED and discover a therapeutic strategy to reduce corneal inflammation. <i>Material and Methods</i>: We used a DED mouse model with extraorbital lacrimal gland (ELG) excision and treated the mice with a decoction of five traditional medicines: <i>Lycium chinense</i>, <i>Cuscuta chinensis</i>, <i>Senna tora</i>, <i>Ophiopogon japonicus</i>, and <i>Dendrobium nobile</i> for 3 months. The tear osmolarity and the ocular surface staining were evaluated as indicators of DED. Immunohistochemistry was used to detect the level of inflammation on the cornea. <i>Results</i>: After treatment with the decoction for three months, epithelial erosions and desquamation were reduced, the intact of corneal endothelium was maintained, and tear osmolarity was restored in the eyes. The IL-1β-associated inflammatory response was reduced in the cornea in the DED model. <i>Conclusions:</i> These data suggested that a mixture of traditional medicines might be a novel therapy to treat DED.https://www.mdpi.com/1648-9144/58/8/1134dry eye diseaselacrimal gland<i>Lycium chinense</i><i>Cuscuta chinensis</i><i>Senna tora</i><i>Ophiopogon japonicu</i> |
| spellingShingle | Cheng-Chan Yang Jia-Ying Chien Yu-Yau Chou Jhih-Wei Ciou Shun-Ping Huang The Effects of <i>Lycium chinense</i>, <i>Cuscuta chinensis</i>, <i>Senna tora</i>, <i>Ophiopogon japonicus</i>, and <i>Dendrobium nobile</i> Decoction on a Dry Eye Mouse Model Medicina dry eye disease lacrimal gland <i>Lycium chinense</i> <i>Cuscuta chinensis</i> <i>Senna tora</i> <i>Ophiopogon japonicu</i> |
| title | The Effects of <i>Lycium chinense</i>, <i>Cuscuta chinensis</i>, <i>Senna tora</i>, <i>Ophiopogon japonicus</i>, and <i>Dendrobium nobile</i> Decoction on a Dry Eye Mouse Model |
| title_full | The Effects of <i>Lycium chinense</i>, <i>Cuscuta chinensis</i>, <i>Senna tora</i>, <i>Ophiopogon japonicus</i>, and <i>Dendrobium nobile</i> Decoction on a Dry Eye Mouse Model |
| title_fullStr | The Effects of <i>Lycium chinense</i>, <i>Cuscuta chinensis</i>, <i>Senna tora</i>, <i>Ophiopogon japonicus</i>, and <i>Dendrobium nobile</i> Decoction on a Dry Eye Mouse Model |
| title_full_unstemmed | The Effects of <i>Lycium chinense</i>, <i>Cuscuta chinensis</i>, <i>Senna tora</i>, <i>Ophiopogon japonicus</i>, and <i>Dendrobium nobile</i> Decoction on a Dry Eye Mouse Model |
| title_short | The Effects of <i>Lycium chinense</i>, <i>Cuscuta chinensis</i>, <i>Senna tora</i>, <i>Ophiopogon japonicus</i>, and <i>Dendrobium nobile</i> Decoction on a Dry Eye Mouse Model |
| title_sort | effects of i lycium chinense i i cuscuta chinensis i i senna tora i i ophiopogon japonicus i and i dendrobium nobile i decoction on a dry eye mouse model |
| topic | dry eye disease lacrimal gland <i>Lycium chinense</i> <i>Cuscuta chinensis</i> <i>Senna tora</i> <i>Ophiopogon japonicu</i> |
| url | https://www.mdpi.com/1648-9144/58/8/1134 |
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