Expression Analysis and Nuclear Import Study of Full-length Isoforms Importin α as 6x Histidin-tagged Fusion Protein on the Intracellular Localization of Recombinant HBV Core Protein
Isoform importin α molecules play a central role in the classical nuclear import pathway, that occurs through the nuclear pore complex (NPC) and typically requires a specific nuclear localization signal (NLS). In this study, it was investigated the role of isoforms importin α in the nuclear import o...
| Main Author: | |
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| Format: | Article |
| Language: | English |
| Published: |
Universitas Gadjah Mada, Yogyakarta
2005-06-01
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| Series: | Indonesian Journal of Biotechnology |
| Online Access: | https://jurnal.ugm.ac.id/ijbiotech/article/view/7412 |
| _version_ | 1828239199269027840 |
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| author | Aris Haryanto |
| author_facet | Aris Haryanto |
| author_sort | Aris Haryanto |
| collection | DOAJ |
| description | Isoform importin α molecules play a central role in the classical nuclear import pathway, that occurs through
the nuclear pore complex (NPC) and typically requires a specific nuclear localization signal (NLS). In this study,
it was investigated the role of isoforms importin α in the nuclear import of wild type recombinant hepatitis B virus
core protein (WT rHBc), phosphorylated recombinant HBV core (rHBc) and recombinant HBV core without NLS
by co-immunoprecipitation. Four recombinant full-length isoforms importin α as 6x histidin-tagged fusion protein
were expressed and analysed from expression plasmid vectors Rch1, pHM 1969, pHM 1967 and pHM 1965. The
results indicated that importin α-1, importin α-3, importin α-4 and importin α-5 can be expressed and isolated
from E. coli transformed recombinant DNA plasmid as protein in size around 58-60 kDa. By the nuclear transport
study shown that isoforms importin α are involved in the nuclear import of WT rHBc, phosphorylated rHBc and
rHBc without NLS. It also indicated that they have an important role for nuclear transport of from cytoplasm into
the nucleus.
Keywords: NPC, NLS, importin α, importin β, isoforms importin α as 6x histidin-tagged fusion protein, WT
rHBc, SV40 Tag, co-immunoprecipitation, westernblotting. |
| first_indexed | 2024-04-12T21:18:50Z |
| format | Article |
| id | doaj.art-13b867114bc34e1492a8e541a186582c |
| institution | Directory Open Access Journal |
| issn | 0853-8654 2089-2241 |
| language | English |
| last_indexed | 2024-04-12T21:18:50Z |
| publishDate | 2005-06-01 |
| publisher | Universitas Gadjah Mada, Yogyakarta |
| record_format | Article |
| series | Indonesian Journal of Biotechnology |
| spelling | doaj.art-13b867114bc34e1492a8e541a186582c2022-12-22T03:16:21ZengUniversitas Gadjah Mada, YogyakartaIndonesian Journal of Biotechnology0853-86542089-22412005-06-0110110.22146/ijbiotech.74126245Expression Analysis and Nuclear Import Study of Full-length Isoforms Importin α as 6x Histidin-tagged Fusion Protein on the Intracellular Localization of Recombinant HBV Core ProteinAris HaryantoIsoform importin α molecules play a central role in the classical nuclear import pathway, that occurs through the nuclear pore complex (NPC) and typically requires a specific nuclear localization signal (NLS). In this study, it was investigated the role of isoforms importin α in the nuclear import of wild type recombinant hepatitis B virus core protein (WT rHBc), phosphorylated recombinant HBV core (rHBc) and recombinant HBV core without NLS by co-immunoprecipitation. Four recombinant full-length isoforms importin α as 6x histidin-tagged fusion protein were expressed and analysed from expression plasmid vectors Rch1, pHM 1969, pHM 1967 and pHM 1965. The results indicated that importin α-1, importin α-3, importin α-4 and importin α-5 can be expressed and isolated from E. coli transformed recombinant DNA plasmid as protein in size around 58-60 kDa. By the nuclear transport study shown that isoforms importin α are involved in the nuclear import of WT rHBc, phosphorylated rHBc and rHBc without NLS. It also indicated that they have an important role for nuclear transport of from cytoplasm into the nucleus. Keywords: NPC, NLS, importin α, importin β, isoforms importin α as 6x histidin-tagged fusion protein, WT rHBc, SV40 Tag, co-immunoprecipitation, westernblotting.https://jurnal.ugm.ac.id/ijbiotech/article/view/7412 |
| spellingShingle | Aris Haryanto Expression Analysis and Nuclear Import Study of Full-length Isoforms Importin α as 6x Histidin-tagged Fusion Protein on the Intracellular Localization of Recombinant HBV Core Protein Indonesian Journal of Biotechnology |
| title | Expression Analysis and Nuclear Import Study of Full-length Isoforms Importin α as 6x Histidin-tagged Fusion Protein on the Intracellular Localization of Recombinant HBV Core Protein |
| title_full | Expression Analysis and Nuclear Import Study of Full-length Isoforms Importin α as 6x Histidin-tagged Fusion Protein on the Intracellular Localization of Recombinant HBV Core Protein |
| title_fullStr | Expression Analysis and Nuclear Import Study of Full-length Isoforms Importin α as 6x Histidin-tagged Fusion Protein on the Intracellular Localization of Recombinant HBV Core Protein |
| title_full_unstemmed | Expression Analysis and Nuclear Import Study of Full-length Isoforms Importin α as 6x Histidin-tagged Fusion Protein on the Intracellular Localization of Recombinant HBV Core Protein |
| title_short | Expression Analysis and Nuclear Import Study of Full-length Isoforms Importin α as 6x Histidin-tagged Fusion Protein on the Intracellular Localization of Recombinant HBV Core Protein |
| title_sort | expression analysis and nuclear import study of full length isoforms importin α as 6x histidin tagged fusion protein on the intracellular localization of recombinant hbv core protein |
| url | https://jurnal.ugm.ac.id/ijbiotech/article/view/7412 |
| work_keys_str_mv | AT arisharyanto expressionanalysisandnuclearimportstudyoffulllengthisoformsimportinaas6xhistidintaggedfusionproteinontheintracellularlocalizationofrecombinanthbvcoreprotein |