Identifying the key genes regulating mesenchymal stem cells chondrogenic differentiation: an in vitro study
Abstract Background Mesenchymal stem cells (MSCs) possess the potential to differentiate into chondrocytes, which makes them an ideal source for healing cartilage defects. Here, we seek to identify the essential genes participating in MSCs chondrogenesis. Methods Human MSCs were induced for chondrog...
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| Format: | Article |
| Language: | English |
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BMC
2022-11-01
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| Series: | BMC Musculoskeletal Disorders |
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| Online Access: | https://doi.org/10.1186/s12891-022-05958-7 |
| _version_ | 1811217774463156224 |
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| author | Tongzhou Liang Pengfei Li Anjing Liang Yuanxin Zhu Xianjian Qiu Jincheng Qiu Yan Peng Dongsheng Huang Wenjie Gao Bo Gao |
| author_facet | Tongzhou Liang Pengfei Li Anjing Liang Yuanxin Zhu Xianjian Qiu Jincheng Qiu Yan Peng Dongsheng Huang Wenjie Gao Bo Gao |
| author_sort | Tongzhou Liang |
| collection | DOAJ |
| description | Abstract Background Mesenchymal stem cells (MSCs) possess the potential to differentiate into chondrocytes, which makes them an ideal source for healing cartilage defects. Here, we seek to identify the essential genes participating in MSCs chondrogenesis. Methods Human MSCs were induced for chondrogenesis for 7, 14, and 21 days using a high-density micromass culture system, and RNA was extracted for RNA-seq. Results A total of 6247 differentially expressed genes (DEGs) were identified on day 7, and 85 DEGs were identified on day 14. However, no significant DEGs was identified on day 21. The top 30 DEGs at day 7, including COL9A3, COL10A1, and CILP2, are closely related to extracellular matrix organization. While the top 30 DEGs at day 14 revealed that inflammation-related genes were enriched, including CXCL8, TLR2, and CCL20. We also conducted protein–protein interaction (PPI) networks analysis using the search tool for the retrieval of interacting genes (STRING) database and identified key hub genes, including CXCL8, TLR2, CCL20, and MMP3. The transcriptional factors were also analyzed, identifying the top 5 TFs: LEF1, FOXO1, RORA, BHLHE41, and SOX5. We demonstrated one particular TF, RORA, in promoting early MSCs chondrogenesis. Conclusions Taken together, our results suggested that these DEGs may have a complex effect on MSCs chondrogenesis both synergistically and solitarily. |
| first_indexed | 2024-04-12T07:00:01Z |
| format | Article |
| id | doaj.art-13b891e07fc84d228589f823c62356c5 |
| institution | Directory Open Access Journal |
| issn | 1471-2474 |
| language | English |
| last_indexed | 2024-04-12T07:00:01Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Musculoskeletal Disorders |
| spelling | doaj.art-13b891e07fc84d228589f823c62356c52022-12-22T03:43:01ZengBMCBMC Musculoskeletal Disorders1471-24742022-11-0123111210.1186/s12891-022-05958-7Identifying the key genes regulating mesenchymal stem cells chondrogenic differentiation: an in vitro studyTongzhou Liang0Pengfei Li1Anjing Liang2Yuanxin Zhu3Xianjian Qiu4Jincheng Qiu5Yan Peng6Dongsheng Huang7Wenjie Gao8Bo Gao9Department of Orthopedics, Sun Yat-Sen Memorial Hospital of Sun Yat-Sen UniversityDepartment of Orthopedics, Sun Yat-Sen Memorial Hospital of Sun Yat-Sen UniversityDepartment of Orthopedics, Sun Yat-Sen Memorial Hospital of Sun Yat-Sen UniversityDepartment of Orthopedics, Sun Yat-Sen Memorial Hospital of Sun Yat-Sen UniversityDepartment of Orthopedics, Sun Yat-Sen Memorial Hospital of Sun Yat-Sen UniversityDepartment of Orthopedics, Sun Yat-Sen Memorial Hospital of Sun Yat-Sen UniversityDepartment of Orthopedics, Sun Yat-Sen Memorial Hospital of Sun Yat-Sen UniversityDepartment of Orthopedics, Sun Yat-Sen Memorial Hospital of Sun Yat-Sen UniversityDepartment of Orthopedics, Sun Yat-Sen Memorial Hospital of Sun Yat-Sen UniversityDepartment of Orthopedics, Sun Yat-Sen Memorial Hospital of Sun Yat-Sen UniversityAbstract Background Mesenchymal stem cells (MSCs) possess the potential to differentiate into chondrocytes, which makes them an ideal source for healing cartilage defects. Here, we seek to identify the essential genes participating in MSCs chondrogenesis. Methods Human MSCs were induced for chondrogenesis for 7, 14, and 21 days using a high-density micromass culture system, and RNA was extracted for RNA-seq. Results A total of 6247 differentially expressed genes (DEGs) were identified on day 7, and 85 DEGs were identified on day 14. However, no significant DEGs was identified on day 21. The top 30 DEGs at day 7, including COL9A3, COL10A1, and CILP2, are closely related to extracellular matrix organization. While the top 30 DEGs at day 14 revealed that inflammation-related genes were enriched, including CXCL8, TLR2, and CCL20. We also conducted protein–protein interaction (PPI) networks analysis using the search tool for the retrieval of interacting genes (STRING) database and identified key hub genes, including CXCL8, TLR2, CCL20, and MMP3. The transcriptional factors were also analyzed, identifying the top 5 TFs: LEF1, FOXO1, RORA, BHLHE41, and SOX5. We demonstrated one particular TF, RORA, in promoting early MSCs chondrogenesis. Conclusions Taken together, our results suggested that these DEGs may have a complex effect on MSCs chondrogenesis both synergistically and solitarily.https://doi.org/10.1186/s12891-022-05958-7Mesenchymal Stem CellsChondrogenesisTranscriptome AnalysisTranscriptional Factors |
| spellingShingle | Tongzhou Liang Pengfei Li Anjing Liang Yuanxin Zhu Xianjian Qiu Jincheng Qiu Yan Peng Dongsheng Huang Wenjie Gao Bo Gao Identifying the key genes regulating mesenchymal stem cells chondrogenic differentiation: an in vitro study BMC Musculoskeletal Disorders Mesenchymal Stem Cells Chondrogenesis Transcriptome Analysis Transcriptional Factors |
| title | Identifying the key genes regulating mesenchymal stem cells chondrogenic differentiation: an in vitro study |
| title_full | Identifying the key genes regulating mesenchymal stem cells chondrogenic differentiation: an in vitro study |
| title_fullStr | Identifying the key genes regulating mesenchymal stem cells chondrogenic differentiation: an in vitro study |
| title_full_unstemmed | Identifying the key genes regulating mesenchymal stem cells chondrogenic differentiation: an in vitro study |
| title_short | Identifying the key genes regulating mesenchymal stem cells chondrogenic differentiation: an in vitro study |
| title_sort | identifying the key genes regulating mesenchymal stem cells chondrogenic differentiation an in vitro study |
| topic | Mesenchymal Stem Cells Chondrogenesis Transcriptome Analysis Transcriptional Factors |
| url | https://doi.org/10.1186/s12891-022-05958-7 |
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