Identification of the RNA-binding domain-containing protein RbpA that acts as a global regulator of the pathogenicity of Streptococcus suis serotype 2
Streptococcus suis serotype 2 (SS2), an emerging zoonotic pathogen, causes swine diseases and human cases of streptococcal toxic shock syndrome. RNA-binding proteins (RBPs) can modulate gene expression through post-transcriptional regulation. In this study, we identified an RBP harbouring an S1 doma...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Virulence |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21505594.2022.2103233 |
| _version_ | 1818160312955699200 |
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| author | Xiaojun Zhong Jiale Ma Qiankun Bai Yinchu Zhu Yue Zhang Qibing Gu Zihao Pan Guangjin Liu Zongfu Wu Huochun Yao |
| author_facet | Xiaojun Zhong Jiale Ma Qiankun Bai Yinchu Zhu Yue Zhang Qibing Gu Zihao Pan Guangjin Liu Zongfu Wu Huochun Yao |
| author_sort | Xiaojun Zhong |
| collection | DOAJ |
| description | Streptococcus suis serotype 2 (SS2), an emerging zoonotic pathogen, causes swine diseases and human cases of streptococcal toxic shock syndrome. RNA-binding proteins (RBPs) can modulate gene expression through post-transcriptional regulation. In this study, we identified an RBP harbouring an S1 domain, named RbpA, which facilitated SS2 adhesion to host epithelial cells and contributed to bacterial pathogenicity. Comparative proteomic analysis identified 145 proteins that were expressed differentially between ΔrbpA strain and wild-type strain, including several virulence-associated factors, such as the extracellular protein factor (EF), SrtF pilus, IgA1 protease, SBP2 pilus, and peptidoglycan-binding LysM’ proteins. The mechanisms underlying the regulatory effects of RbpA on their encoding genes were explored, and it was found that RbpA regulates gene expression through diverse mechanisms, including post-transcriptional regulation, and thus acts as a global regulator. These results partly reveal the pathogenic mechanism mediated by RbpA, improving our understanding of the regulatory systems of S. suis and providing new insights into bacterial pathogenicity. |
| first_indexed | 2024-12-11T15:59:53Z |
| format | Article |
| id | doaj.art-13bb745987244d21afe16ba8bb6ab2cd |
| institution | Directory Open Access Journal |
| issn | 2150-5594 2150-5608 |
| language | English |
| last_indexed | 2024-12-11T15:59:53Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj.art-13bb745987244d21afe16ba8bb6ab2cd2022-12-22T00:59:20ZengTaylor & Francis GroupVirulence2150-55942150-56082022-12-011311304131410.1080/21505594.2022.2103233Identification of the RNA-binding domain-containing protein RbpA that acts as a global regulator of the pathogenicity of Streptococcus suis serotype 2Xiaojun Zhong0Jiale Ma1Qiankun Bai2Yinchu Zhu3Yue Zhang4Qibing Gu5Zihao Pan6Guangjin Liu7Zongfu Wu8Huochun Yao9College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A & F University, Hangzhou, ChinaOIE Reference Lab for Swine Streptococcosis, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaOIE Reference Lab for Swine Streptococcosis, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaOIE Reference Lab for Swine Streptococcosis, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaOIE Reference Lab for Swine Streptococcosis, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaOIE Reference Lab for Swine Streptococcosis, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaOIE Reference Lab for Swine Streptococcosis, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaOIE Reference Lab for Swine Streptococcosis, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaOIE Reference Lab for Swine Streptococcosis, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaOIE Reference Lab for Swine Streptococcosis, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaStreptococcus suis serotype 2 (SS2), an emerging zoonotic pathogen, causes swine diseases and human cases of streptococcal toxic shock syndrome. RNA-binding proteins (RBPs) can modulate gene expression through post-transcriptional regulation. In this study, we identified an RBP harbouring an S1 domain, named RbpA, which facilitated SS2 adhesion to host epithelial cells and contributed to bacterial pathogenicity. Comparative proteomic analysis identified 145 proteins that were expressed differentially between ΔrbpA strain and wild-type strain, including several virulence-associated factors, such as the extracellular protein factor (EF), SrtF pilus, IgA1 protease, SBP2 pilus, and peptidoglycan-binding LysM’ proteins. The mechanisms underlying the regulatory effects of RbpA on their encoding genes were explored, and it was found that RbpA regulates gene expression through diverse mechanisms, including post-transcriptional regulation, and thus acts as a global regulator. These results partly reveal the pathogenic mechanism mediated by RbpA, improving our understanding of the regulatory systems of S. suis and providing new insights into bacterial pathogenicity.https://www.tandfonline.com/doi/10.1080/21505594.2022.2103233Streptococcus suisRNA-binding proteinRbpapathogenicitypost-transcriptional regulation |
| spellingShingle | Xiaojun Zhong Jiale Ma Qiankun Bai Yinchu Zhu Yue Zhang Qibing Gu Zihao Pan Guangjin Liu Zongfu Wu Huochun Yao Identification of the RNA-binding domain-containing protein RbpA that acts as a global regulator of the pathogenicity of Streptococcus suis serotype 2 Virulence Streptococcus suis RNA-binding protein Rbpa pathogenicity post-transcriptional regulation |
| title | Identification of the RNA-binding domain-containing protein RbpA that acts as a global regulator of the pathogenicity of Streptococcus suis serotype 2 |
| title_full | Identification of the RNA-binding domain-containing protein RbpA that acts as a global regulator of the pathogenicity of Streptococcus suis serotype 2 |
| title_fullStr | Identification of the RNA-binding domain-containing protein RbpA that acts as a global regulator of the pathogenicity of Streptococcus suis serotype 2 |
| title_full_unstemmed | Identification of the RNA-binding domain-containing protein RbpA that acts as a global regulator of the pathogenicity of Streptococcus suis serotype 2 |
| title_short | Identification of the RNA-binding domain-containing protein RbpA that acts as a global regulator of the pathogenicity of Streptococcus suis serotype 2 |
| title_sort | identification of the rna binding domain containing protein rbpa that acts as a global regulator of the pathogenicity of streptococcus suis serotype 2 |
| topic | Streptococcus suis RNA-binding protein Rbpa pathogenicity post-transcriptional regulation |
| url | https://www.tandfonline.com/doi/10.1080/21505594.2022.2103233 |
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