Development of in vitro culture system for proliferation of protocormlike bodies of Oncidium Golden Anniversary orchid

Development of in vitro culture system for proliferation of protocormlike bodies of Oncidium Golden Anniversary orchid

Oncidium orchid has high demand and economic value especially in floriculture industry. This study aimed to optimize a suitable medium for rapid proliferation of protocorm-like bodies (PLBs) of Oncidium Golden Anniversary orchid. Results from this study confirmed that ½ MS semi-solid medium displa...

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Bibliographic Details
Main Authors: Suhana Zakaria, Jessica Jeyanthi James Antony, Chee Keong Chin, Rahmad Zakaria, Sreeramanan Subramaniam
Format: Article
Language:English
Published: Prince of Songkla University 2021-12-01
Series:Songklanakarin Journal of Science and Technology (SJST)
Subjects:
oncidium golden anniversary
protocorm-like bodies
growth index
thin cell layer
Online Access:https://rdo.psu.ac.th/sjst/journal/43-6/1.pdf
Description
Summary:Oncidium orchid has high demand and economic value especially in floriculture industry. This study aimed to optimize a suitable medium for rapid proliferation of protocorm-like bodies (PLBs) of Oncidium Golden Anniversary orchid. Results from this study confirmed that ½ MS semi-solid medium displayed the highest growth index and total numbers of PLBs. Inclusion of 20g/L sucrose and 1mg/L BAP produced the highest PLB proliferation rate, while adding charcoal gave no significant effect. Half-moon PLB size contributes to the highest growth rate on using a thin cell layer (TCL) system. Hence, the best medium for Oncidium Golden Anniversary PLB proliferation was ½ MS semi-solid media supplemented with 20 g/L sucrose and 1 mg/L BAP.
ISSN:0125-3395

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