Phosphoprotein Profile of Rice (<i>Oryza sativa</i> L.) Seedlings under Osmotic Stress after Pretreatment with Chitosan
This study aims to identify novel chitosan (CTS)-responsive phosphoproteins in Leung Pratew 123 (LPT123) and Khao Dawk Mali 105 (KDML105) as drought-sensitive rice cultivars and differences in the CTS response. Rice seeds were soaked in CTS solution before germination, and 2- and 4-week-old rice see...
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MDPI AG
2022-10-01
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| Online Access: | https://www.mdpi.com/2223-7747/11/20/2729 |
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| author | Wasinee Pongprayoon Atikorn Panya Janthima Jaresitthikunchai Narumon Phaonakrop Sittiruk Roytrakul |
| author_facet | Wasinee Pongprayoon Atikorn Panya Janthima Jaresitthikunchai Narumon Phaonakrop Sittiruk Roytrakul |
| author_sort | Wasinee Pongprayoon |
| collection | DOAJ |
| description | This study aims to identify novel chitosan (CTS)-responsive phosphoproteins in Leung Pratew 123 (LPT123) and Khao Dawk Mali 105 (KDML105) as drought-sensitive rice cultivars and differences in the CTS response. Rice seeds were soaked in CTS solution before germination, and 2- and 4-week-old rice seedlings sprayed with CTS before osmotic stress comprised the following four groups: (1) seedlings treated with distilled water; (2) seedlings treated with CTS; (3) seedlings pretreated with distilled water and subjected to osmotic stress; and (4) seedlings pretreated with CTS and subjected to osmotic stress. Phosphoproteins of leaf tissues were enriched using immobilized metal affinity chromatography (IMAC) before tryptic digestion and analysis via LC-MS. Phosphoprotein profiling analyses led to the identification of 4721 phosphoproteins representing 1052 and 1040 unique phosphoproteins in the LPT123 and KDML105 seedlings, respectively. In response to CTS pretreatment before osmotic stress, 22 differently expressed proteins were discovered, of which 10 and 12 were identified in the LPT123 and KDML105, respectively. These proteins are typically involved in signaling, transport, protein folding, protein degradation, and metabolism. This study provides fruitful data to understand the signal transduction mechanisms of rice seedlings pretreated with CTS before exposure to osmotic stress. |
| first_indexed | 2024-03-09T19:33:18Z |
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| id | doaj.art-13c11737be634d9e97a977e50a8a8a90 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T19:33:18Z |
| publishDate | 2022-10-01 |
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| series | Plants |
| spelling | doaj.art-13c11737be634d9e97a977e50a8a8a902023-11-24T02:04:19ZengMDPI AGPlants2223-77472022-10-011120272910.3390/plants11202729Phosphoprotein Profile of Rice (<i>Oryza sativa</i> L.) Seedlings under Osmotic Stress after Pretreatment with ChitosanWasinee Pongprayoon0Atikorn Panya1Janthima Jaresitthikunchai2Narumon Phaonakrop3Sittiruk Roytrakul4Department of Biology, Faculty of Science, Burapha University, 169 Longhaad Bangsaen Rd, Saensook, Mueang, Chonburi 20131, ThailandFunctional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Phaholyothin Rd., Klong Luang, Pathum Thani 12120, ThailandFunctional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Phaholyothin Rd., Klong Luang, Pathum Thani 12120, ThailandFunctional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Phaholyothin Rd., Klong Luang, Pathum Thani 12120, ThailandFunctional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Phaholyothin Rd., Klong Luang, Pathum Thani 12120, ThailandThis study aims to identify novel chitosan (CTS)-responsive phosphoproteins in Leung Pratew 123 (LPT123) and Khao Dawk Mali 105 (KDML105) as drought-sensitive rice cultivars and differences in the CTS response. Rice seeds were soaked in CTS solution before germination, and 2- and 4-week-old rice seedlings sprayed with CTS before osmotic stress comprised the following four groups: (1) seedlings treated with distilled water; (2) seedlings treated with CTS; (3) seedlings pretreated with distilled water and subjected to osmotic stress; and (4) seedlings pretreated with CTS and subjected to osmotic stress. Phosphoproteins of leaf tissues were enriched using immobilized metal affinity chromatography (IMAC) before tryptic digestion and analysis via LC-MS. Phosphoprotein profiling analyses led to the identification of 4721 phosphoproteins representing 1052 and 1040 unique phosphoproteins in the LPT123 and KDML105 seedlings, respectively. In response to CTS pretreatment before osmotic stress, 22 differently expressed proteins were discovered, of which 10 and 12 were identified in the LPT123 and KDML105, respectively. These proteins are typically involved in signaling, transport, protein folding, protein degradation, and metabolism. This study provides fruitful data to understand the signal transduction mechanisms of rice seedlings pretreated with CTS before exposure to osmotic stress.https://www.mdpi.com/2223-7747/11/20/2729phosphoproteomicschitosanosmotic stressKhao Dawk Mali 105Leung Pratew 123rice |
| spellingShingle | Wasinee Pongprayoon Atikorn Panya Janthima Jaresitthikunchai Narumon Phaonakrop Sittiruk Roytrakul Phosphoprotein Profile of Rice (<i>Oryza sativa</i> L.) Seedlings under Osmotic Stress after Pretreatment with Chitosan Plants phosphoproteomics chitosan osmotic stress Khao Dawk Mali 105 Leung Pratew 123 rice |
| title | Phosphoprotein Profile of Rice (<i>Oryza sativa</i> L.) Seedlings under Osmotic Stress after Pretreatment with Chitosan |
| title_full | Phosphoprotein Profile of Rice (<i>Oryza sativa</i> L.) Seedlings under Osmotic Stress after Pretreatment with Chitosan |
| title_fullStr | Phosphoprotein Profile of Rice (<i>Oryza sativa</i> L.) Seedlings under Osmotic Stress after Pretreatment with Chitosan |
| title_full_unstemmed | Phosphoprotein Profile of Rice (<i>Oryza sativa</i> L.) Seedlings under Osmotic Stress after Pretreatment with Chitosan |
| title_short | Phosphoprotein Profile of Rice (<i>Oryza sativa</i> L.) Seedlings under Osmotic Stress after Pretreatment with Chitosan |
| title_sort | phosphoprotein profile of rice i oryza sativa i l seedlings under osmotic stress after pretreatment with chitosan |
| topic | phosphoproteomics chitosan osmotic stress Khao Dawk Mali 105 Leung Pratew 123 rice |
| url | https://www.mdpi.com/2223-7747/11/20/2729 |
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