Exogenous spermidine enhanced the water deficit tolerance of Anoectochilus roxburghii by modulating plant antioxidant enzymes and polyamine metabolism
Water stress is an important factor affecting herb quality and yield and exogenous spermidine (Spd) addition can enhance dehydration tolerance of plants. Anoectochilus roxburghii (Wall.) Lindl is a perennial herb widely used in medicine, health care, and beauty industry, yet, its dehydration resista...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Agricultural Water Management |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0378377423004031 |
| _version_ | 1797637640856535040 |
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| author | Xutong Sun Aimin Lv Dandan Chen Zili Zhang Xuming Wang Aicun Zhou Xiaowei Xu Qingsong Shao Ying Zheng |
| author_facet | Xutong Sun Aimin Lv Dandan Chen Zili Zhang Xuming Wang Aicun Zhou Xiaowei Xu Qingsong Shao Ying Zheng |
| author_sort | Xutong Sun |
| collection | DOAJ |
| description | Water stress is an important factor affecting herb quality and yield and exogenous spermidine (Spd) addition can enhance dehydration tolerance of plants. Anoectochilus roxburghii (Wall.) Lindl is a perennial herb widely used in medicine, health care, and beauty industry, yet, its dehydration resistance remains largely unknown. In this view, the current research aimed at elucidating the drought resistance mechanism of A. roxburghii under PEG-induced water stress at different levels (10%, 20%, and 30% PEG6000). In particular, the morphological, physiological, and biochemical changes occurring in leaves treated with (0.5 mM, 5 mM) or without Spd were discussed under moderate water stress (20% PEG6000). Results showed that water stress significantly inhibited the growth of A. roxburghii by decreasing the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), arginine decarboxylase (ADC), S-adenosylmethionine decarboxylase (SAMDC), and polyamine oxidase (PAO). The contents of soluble protein, soluble proline, hydrogen peroxide (H2O2), malondialdehyde (MDA), and the relative conductivity (REC) of leaves increased by 27.32%, 31.37%, 68.84%, 103.61%, and 63.64%, respectively. Under dehydration condition, foliar spraying Spd effectively improved plant growth with the contents of soluble protein and soluble proline increased, while the H2O2, REC, and MDA contents decreased. The SOD activity of 0.5 mM and 5 mM treatments were 1.13 and 1.01 times higher than only 20% PEG treatment, respectively. Similarly, the POD activity of 0.5 mM and 5 mM treatments increased by 52.82% and 34.11%, while the CAT activity of 0.5 mM and 5 mM treatments increased by 34.39 and 33.15 U/mgprot, respectively. In addition, exogenous Spd application significantly increased the key enzyme activities of polyamine metabolism (ADC, SAMDC, and PAO) under moderate water stress. In conclusion, exogenous Spd addition with low concentration (0.5 mM) improved the resistance of A. roxburghii against water stress by increasing the antioxidant enzymes activities and endogenous polyamines contents. This study helps to the understanding of dehydration resistance of A. roxburghii and provide guidelines for its cultivation under water deficit condition. |
| first_indexed | 2024-03-11T12:51:17Z |
| format | Article |
| id | doaj.art-13c125422a5b4b0db9f1dc28ae637f92 |
| institution | Directory Open Access Journal |
| issn | 1873-2283 |
| language | English |
| last_indexed | 2024-03-11T12:51:17Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Agricultural Water Management |
| spelling | doaj.art-13c125422a5b4b0db9f1dc28ae637f922023-11-04T04:17:19ZengElsevierAgricultural Water Management1873-22832023-11-01289108538Exogenous spermidine enhanced the water deficit tolerance of Anoectochilus roxburghii by modulating plant antioxidant enzymes and polyamine metabolismXutong Sun0Aimin Lv1Dandan Chen2Zili Zhang3Xuming Wang4Aicun Zhou5Xiaowei Xu6Qingsong Shao7Ying Zheng8State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Hangzhou 311300, China; Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine, Zhejiang A & F University, Hangzhou 311300, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Hangzhou 311300, China; Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine, Zhejiang A & F University, Hangzhou 311300, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Hangzhou 311300, China; Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine, Zhejiang A & F University, Hangzhou 311300, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Hangzhou 311300, China; Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine, Zhejiang A & F University, Hangzhou 311300, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Hangzhou 311300, China; Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine, Zhejiang A & F University, Hangzhou 311300, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Hangzhou 311300, China; Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine, Zhejiang A & F University, Hangzhou 311300, ChinaWenzhou Forestry Technology Promotion and Wildlife Protection and Management Station, Wenzhou, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Hangzhou 311300, China; Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine, Zhejiang A & F University, Hangzhou 311300, China; Corresponding authors at: State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Hangzhou 311300, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Hangzhou 311300, China; Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine, Zhejiang A & F University, Hangzhou 311300, China; Corresponding authors at: State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Hangzhou 311300, ChinaWater stress is an important factor affecting herb quality and yield and exogenous spermidine (Spd) addition can enhance dehydration tolerance of plants. Anoectochilus roxburghii (Wall.) Lindl is a perennial herb widely used in medicine, health care, and beauty industry, yet, its dehydration resistance remains largely unknown. In this view, the current research aimed at elucidating the drought resistance mechanism of A. roxburghii under PEG-induced water stress at different levels (10%, 20%, and 30% PEG6000). In particular, the morphological, physiological, and biochemical changes occurring in leaves treated with (0.5 mM, 5 mM) or without Spd were discussed under moderate water stress (20% PEG6000). Results showed that water stress significantly inhibited the growth of A. roxburghii by decreasing the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), arginine decarboxylase (ADC), S-adenosylmethionine decarboxylase (SAMDC), and polyamine oxidase (PAO). The contents of soluble protein, soluble proline, hydrogen peroxide (H2O2), malondialdehyde (MDA), and the relative conductivity (REC) of leaves increased by 27.32%, 31.37%, 68.84%, 103.61%, and 63.64%, respectively. Under dehydration condition, foliar spraying Spd effectively improved plant growth with the contents of soluble protein and soluble proline increased, while the H2O2, REC, and MDA contents decreased. The SOD activity of 0.5 mM and 5 mM treatments were 1.13 and 1.01 times higher than only 20% PEG treatment, respectively. Similarly, the POD activity of 0.5 mM and 5 mM treatments increased by 52.82% and 34.11%, while the CAT activity of 0.5 mM and 5 mM treatments increased by 34.39 and 33.15 U/mgprot, respectively. In addition, exogenous Spd application significantly increased the key enzyme activities of polyamine metabolism (ADC, SAMDC, and PAO) under moderate water stress. In conclusion, exogenous Spd addition with low concentration (0.5 mM) improved the resistance of A. roxburghii against water stress by increasing the antioxidant enzymes activities and endogenous polyamines contents. This study helps to the understanding of dehydration resistance of A. roxburghii and provide guidelines for its cultivation under water deficit condition.http://www.sciencedirect.com/science/article/pii/S0378377423004031Anoectochilus roxburghiiPhysiological responsePlant growthSpermidineWater stress |
| spellingShingle | Xutong Sun Aimin Lv Dandan Chen Zili Zhang Xuming Wang Aicun Zhou Xiaowei Xu Qingsong Shao Ying Zheng Exogenous spermidine enhanced the water deficit tolerance of Anoectochilus roxburghii by modulating plant antioxidant enzymes and polyamine metabolism Agricultural Water Management Anoectochilus roxburghii Physiological response Plant growth Spermidine Water stress |
| title | Exogenous spermidine enhanced the water deficit tolerance of Anoectochilus roxburghii by modulating plant antioxidant enzymes and polyamine metabolism |
| title_full | Exogenous spermidine enhanced the water deficit tolerance of Anoectochilus roxburghii by modulating plant antioxidant enzymes and polyamine metabolism |
| title_fullStr | Exogenous spermidine enhanced the water deficit tolerance of Anoectochilus roxburghii by modulating plant antioxidant enzymes and polyamine metabolism |
| title_full_unstemmed | Exogenous spermidine enhanced the water deficit tolerance of Anoectochilus roxburghii by modulating plant antioxidant enzymes and polyamine metabolism |
| title_short | Exogenous spermidine enhanced the water deficit tolerance of Anoectochilus roxburghii by modulating plant antioxidant enzymes and polyamine metabolism |
| title_sort | exogenous spermidine enhanced the water deficit tolerance of anoectochilus roxburghii by modulating plant antioxidant enzymes and polyamine metabolism |
| topic | Anoectochilus roxburghii Physiological response Plant growth Spermidine Water stress |
| url | http://www.sciencedirect.com/science/article/pii/S0378377423004031 |
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