High-sensitive and rapid detection of <it>Mycobacterium tuberculosis </it>infection by IFN-γ release assay among HIV-infected individuals in BCG-vaccinated area
<p>Abstract</p> <p>Background</p> <p>An accurate test for <it>Mycobacterium tuberculosis </it>infection is urgently needed in immunosuppressed populations. The aim of this study was to investigate the diagnostic power of enzyme-linked immunospot (ELISPOT)-ba...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-05-01
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| Series: | BMC Immunology |
| Online Access: | http://www.biomedcentral.com/1471-2172/10/31 |
| _version_ | 1818155557924634624 |
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| author | Jiang Weimin Shao Lingyun Zhang Ying Zhang Shu Meng Chengyan Xu Yunya Huang Lingli Wang Yun Wang Ying Weng Xinhua Zhang Wenhong |
| author_facet | Jiang Weimin Shao Lingyun Zhang Ying Zhang Shu Meng Chengyan Xu Yunya Huang Lingli Wang Yun Wang Ying Weng Xinhua Zhang Wenhong |
| author_sort | Jiang Weimin |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>An accurate test for <it>Mycobacterium tuberculosis </it>infection is urgently needed in immunosuppressed populations. The aim of this study was to investigate the diagnostic power of enzyme-linked immunospot (ELISPOT)-based IFN-γ release assay in detecting active and latent tuberculosis in HIV-infected population in <it>bacillus Calmette-Guerin </it>(BCG)-vaccinated area. A total of 100 HIV-infected individuals including 32 active tuberculosis patients were recruited. An ELISPOT-based IFN-γ release assay, T-SPOT.TB, was used to evaluate the <it>M. tuberculosis </it>ESAT-6 and CFP-10 specific IFN-γ response. Tuberculin skin test (TST) was performed for all recruited subjects.</p> <p>Results</p> <p>The subjects were divided into group HIV+ATB (HIV-infected individuals with active tuberculosis, n = 32), group HIV+LTB (HIV-infected individuals with positive results of T-SPOT.TB assay, n = 46) and group HIV only (HIV-infected individuals with negative results of T-SPOT.TB assay and without evidence of tuberculosis infection, n = 22). In group HIV+ATB and HIV+LTB, T-SPOT.TB positive rate in subjects with TST <5 mm were 50% (16/32) and 41.3% (19/46), respectively. Individuals in group HIV+ATB and HIV+LTB with CD4+ T cells <500/μl, T-SPOT.TB showed a higher sensitivity than TST (64.5% vs. 22.6% and 62.2% vs. 29.7%, respectively, both <it>P </it>< 0.0001). In addition, the sensitivity of T-SPOT.TB assay in group HIV+ATB increased to >85% in patients with TB treatment for less than 1 month and CD4+ T cells ≥200/μl, while for patients treated for more than 3 months and CD4+ T cells <200/μl, the sensitivity was decreased to only 33.3%. Furthermore, the results could be generated by T-SPOT.TB assay within 24 hours, which was more rapid than TST with 48–72 hours.</p> <p>Conclusion</p> <p>ELISPOT-based IFN-γ release assay is more sensitive and rapid for the diagnosis of TB infection in Chinese HIV-infected individuals with history of BCG vaccination, and could be an effective tool for guiding preventive treatment with isoniazid in latently infected people and for TB control in China.</p> |
| first_indexed | 2024-12-11T14:44:18Z |
| format | Article |
| id | doaj.art-13c15490a2cf4e08ae396aa9471cfe7c |
| institution | Directory Open Access Journal |
| issn | 1471-2172 |
| language | English |
| last_indexed | 2024-12-11T14:44:18Z |
| publishDate | 2009-05-01 |
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| series | BMC Immunology |
| spelling | doaj.art-13c15490a2cf4e08ae396aa9471cfe7c2022-12-22T01:01:46ZengBMCBMC Immunology1471-21722009-05-011013110.1186/1471-2172-10-31High-sensitive and rapid detection of <it>Mycobacterium tuberculosis </it>infection by IFN-γ release assay among HIV-infected individuals in BCG-vaccinated areaJiang WeiminShao LingyunZhang YingZhang ShuMeng ChengyanXu YunyaHuang LingliWang YunWang YingWeng XinhuaZhang Wenhong<p>Abstract</p> <p>Background</p> <p>An accurate test for <it>Mycobacterium tuberculosis </it>infection is urgently needed in immunosuppressed populations. The aim of this study was to investigate the diagnostic power of enzyme-linked immunospot (ELISPOT)-based IFN-γ release assay in detecting active and latent tuberculosis in HIV-infected population in <it>bacillus Calmette-Guerin </it>(BCG)-vaccinated area. A total of 100 HIV-infected individuals including 32 active tuberculosis patients were recruited. An ELISPOT-based IFN-γ release assay, T-SPOT.TB, was used to evaluate the <it>M. tuberculosis </it>ESAT-6 and CFP-10 specific IFN-γ response. Tuberculin skin test (TST) was performed for all recruited subjects.</p> <p>Results</p> <p>The subjects were divided into group HIV+ATB (HIV-infected individuals with active tuberculosis, n = 32), group HIV+LTB (HIV-infected individuals with positive results of T-SPOT.TB assay, n = 46) and group HIV only (HIV-infected individuals with negative results of T-SPOT.TB assay and without evidence of tuberculosis infection, n = 22). In group HIV+ATB and HIV+LTB, T-SPOT.TB positive rate in subjects with TST <5 mm were 50% (16/32) and 41.3% (19/46), respectively. Individuals in group HIV+ATB and HIV+LTB with CD4+ T cells <500/μl, T-SPOT.TB showed a higher sensitivity than TST (64.5% vs. 22.6% and 62.2% vs. 29.7%, respectively, both <it>P </it>< 0.0001). In addition, the sensitivity of T-SPOT.TB assay in group HIV+ATB increased to >85% in patients with TB treatment for less than 1 month and CD4+ T cells ≥200/μl, while for patients treated for more than 3 months and CD4+ T cells <200/μl, the sensitivity was decreased to only 33.3%. Furthermore, the results could be generated by T-SPOT.TB assay within 24 hours, which was more rapid than TST with 48–72 hours.</p> <p>Conclusion</p> <p>ELISPOT-based IFN-γ release assay is more sensitive and rapid for the diagnosis of TB infection in Chinese HIV-infected individuals with history of BCG vaccination, and could be an effective tool for guiding preventive treatment with isoniazid in latently infected people and for TB control in China.</p>http://www.biomedcentral.com/1471-2172/10/31 |
| spellingShingle | Jiang Weimin Shao Lingyun Zhang Ying Zhang Shu Meng Chengyan Xu Yunya Huang Lingli Wang Yun Wang Ying Weng Xinhua Zhang Wenhong High-sensitive and rapid detection of <it>Mycobacterium tuberculosis </it>infection by IFN-γ release assay among HIV-infected individuals in BCG-vaccinated area BMC Immunology |
| title | High-sensitive and rapid detection of <it>Mycobacterium tuberculosis </it>infection by IFN-γ release assay among HIV-infected individuals in BCG-vaccinated area |
| title_full | High-sensitive and rapid detection of <it>Mycobacterium tuberculosis </it>infection by IFN-γ release assay among HIV-infected individuals in BCG-vaccinated area |
| title_fullStr | High-sensitive and rapid detection of <it>Mycobacterium tuberculosis </it>infection by IFN-γ release assay among HIV-infected individuals in BCG-vaccinated area |
| title_full_unstemmed | High-sensitive and rapid detection of <it>Mycobacterium tuberculosis </it>infection by IFN-γ release assay among HIV-infected individuals in BCG-vaccinated area |
| title_short | High-sensitive and rapid detection of <it>Mycobacterium tuberculosis </it>infection by IFN-γ release assay among HIV-infected individuals in BCG-vaccinated area |
| title_sort | high sensitive and rapid detection of it mycobacterium tuberculosis it infection by ifn γ release assay among hiv infected individuals in bcg vaccinated area |
| url | http://www.biomedcentral.com/1471-2172/10/31 |
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