Evaluation of <i>Russian sturgeon</i> (<i>Acipenser gueldenstaedtii</i>) Semen Quality and Semen Cryopreservation
The alarming decline in sturgeon populations doubled by growing demands for sturgeon products, urge us to prevent the loss of these species. Fish stocking in natural habitats and developing fish farms are viable solutions, which can be successfully implemented with the help of reproductive biotechno...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/16/2153 |
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| author | Violeta Igna Ada Telea Tiana Florea Roxana Popp Adrian Grozea |
| author_facet | Violeta Igna Ada Telea Tiana Florea Roxana Popp Adrian Grozea |
| author_sort | Violeta Igna |
| collection | DOAJ |
| description | The alarming decline in sturgeon populations doubled by growing demands for sturgeon products, urge us to prevent the loss of these species. Fish stocking in natural habitats and developing fish farms are viable solutions, which can be successfully implemented with the help of reproductive biotechnologies. Despite the fact that semen cryopreservation is admittedly an important step for saving the Russian sturgeon, a reproducible standard method with good results has yet to be identified. Sperm quality assessment is essential for quantifying the impact of cryopreservation on spermatozoa. The purpose of our study was to provide additional information regarding semen cryopreservation and semen quality evaluation for the Russian sturgeon. Our study method is based on the use of two yolk-free extenders (with different cryoprotectants: DMSO and methanol) for freezing semen, using a simple freezing protocol. Parameters such as volume, concentration, motility, morphology and membrane integrity were evaluated. In conclusion, cryopreservation of Russian sturgeon spermatozoa using an extender containing methanol as cryoprotectant led to high egg fertilization percentages (72.67 ± 5.4%) even if the total motility values recorded for thawed semen were quite low (18–25%). Additionally, we identified two optimal stains for morphological studies and morphometry (Spermac stain kit and Trypan Blue Solution). |
| first_indexed | 2024-03-09T04:47:32Z |
| format | Article |
| id | doaj.art-13c659197b21479a9d92df534bb60c2e |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T04:47:32Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-13c659197b21479a9d92df534bb60c2e2023-12-03T13:13:57ZengMDPI AGAnimals2076-26152022-08-011216215310.3390/ani12162153Evaluation of <i>Russian sturgeon</i> (<i>Acipenser gueldenstaedtii</i>) Semen Quality and Semen CryopreservationVioleta Igna0Ada Telea1Tiana Florea2Roxana Popp3Adrian Grozea4Faculty of Veterinary Medicine, Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timisoara, 300645 Timisoara, RomaniaCenter for Gene and Cellular Therapies in the Treatment of Cancer—OncoGen, Clinical Emergency County Hospital “Pius Brinzeu” Timisoara, 300723 Timisoara, RomaniaFaculty of Veterinary Medicine, Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timisoara, 300645 Timisoara, RomaniaFaculty of Veterinary Medicine, Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timisoara, 300645 Timisoara, RomaniaFaculty of Bioengineering of Animal Resources, Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timisoara, 300645 Timisoara, RomaniaThe alarming decline in sturgeon populations doubled by growing demands for sturgeon products, urge us to prevent the loss of these species. Fish stocking in natural habitats and developing fish farms are viable solutions, which can be successfully implemented with the help of reproductive biotechnologies. Despite the fact that semen cryopreservation is admittedly an important step for saving the Russian sturgeon, a reproducible standard method with good results has yet to be identified. Sperm quality assessment is essential for quantifying the impact of cryopreservation on spermatozoa. The purpose of our study was to provide additional information regarding semen cryopreservation and semen quality evaluation for the Russian sturgeon. Our study method is based on the use of two yolk-free extenders (with different cryoprotectants: DMSO and methanol) for freezing semen, using a simple freezing protocol. Parameters such as volume, concentration, motility, morphology and membrane integrity were evaluated. In conclusion, cryopreservation of Russian sturgeon spermatozoa using an extender containing methanol as cryoprotectant led to high egg fertilization percentages (72.67 ± 5.4%) even if the total motility values recorded for thawed semen were quite low (18–25%). Additionally, we identified two optimal stains for morphological studies and morphometry (Spermac stain kit and Trypan Blue Solution).https://www.mdpi.com/2076-2615/12/16/2153Russian sturgeonsemen evaluationcryopreservationmorphologyfertilization rate |
| spellingShingle | Violeta Igna Ada Telea Tiana Florea Roxana Popp Adrian Grozea Evaluation of <i>Russian sturgeon</i> (<i>Acipenser gueldenstaedtii</i>) Semen Quality and Semen Cryopreservation Animals Russian sturgeon semen evaluation cryopreservation morphology fertilization rate |
| title | Evaluation of <i>Russian sturgeon</i> (<i>Acipenser gueldenstaedtii</i>) Semen Quality and Semen Cryopreservation |
| title_full | Evaluation of <i>Russian sturgeon</i> (<i>Acipenser gueldenstaedtii</i>) Semen Quality and Semen Cryopreservation |
| title_fullStr | Evaluation of <i>Russian sturgeon</i> (<i>Acipenser gueldenstaedtii</i>) Semen Quality and Semen Cryopreservation |
| title_full_unstemmed | Evaluation of <i>Russian sturgeon</i> (<i>Acipenser gueldenstaedtii</i>) Semen Quality and Semen Cryopreservation |
| title_short | Evaluation of <i>Russian sturgeon</i> (<i>Acipenser gueldenstaedtii</i>) Semen Quality and Semen Cryopreservation |
| title_sort | evaluation of i russian sturgeon i i acipenser gueldenstaedtii i semen quality and semen cryopreservation |
| topic | Russian sturgeon semen evaluation cryopreservation morphology fertilization rate |
| url | https://www.mdpi.com/2076-2615/12/16/2153 |
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