Ablation of p120-Catenin Altering the Activity of Small GTPase in Human Lung Cancer Cells
Background and objective p120-catenin (p120ctn), a member of the Armadillo gene family, has emerged as an important modulator of small GTPase activities. Therefore, it plays novel roles in tumor malignant phenotype, such as invasion and metastasis, whose mechanism are not well clarified yet. The aim...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | zho |
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Chinese Anti-Cancer Association; Chinese Antituberculosis Association
2009-05-01
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| Series: | Chinese Journal of Lung Cancer |
| Subjects: | |
| Online Access: | http://www.lungca.org/index.php?journal=01&page=article&op=view&path%5B%5D=10.3779%2Fj.issn.1009-3419.2009.05.002&path%5B%5D=814 |
| _version_ | 1828155184440672256 |
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| author | Nan LIU Yang LIU Shuli LIU Enhua WANG |
| author_facet | Nan LIU Yang LIU Shuli LIU Enhua WANG |
| author_sort | Nan LIU |
| collection | DOAJ |
| description | Background and objective p120-catenin (p120ctn), a member of the Armadillo gene family, has emerged as an important modulator of small GTPase activities. Therefore, it plays novel roles in tumor malignant phenotype, such as invasion and metastasis, whose mechanism are not well clarified yet. The aim of this study is to explore the roles of p120ctn on the regulation of small GTP family members in lung cancer and the effects to lung cancer invasions andmetastasis. Methods After p120ctn was knocked down by siRNA, in vivo and in vitro analysis was applied to investigate the role and possible mechanism of p120ctn in lung cancer, such as Western Blot, pull-down analysis, and nude mice models. Results p120ctn depletion inactivated RhoA, with the the activity of Cdc42 and Rac1 increased, the invasiveness of lung cancer cells was promoted both in vitro and in vivo . Conclusion p120ctn gene knockdown enhances the metastasis of lung cancer cells, probably by altering expression of small GTPase, such as inactivation of RhoA and activation of Cdc42/Rac1. |
| first_indexed | 2024-04-11T22:50:30Z |
| format | Article |
| id | doaj.art-13c6c69d8b2a482cb2dd47d7a6073be5 |
| institution | Directory Open Access Journal |
| issn | 1009-3419 1999-6187 |
| language | zho |
| last_indexed | 2024-04-11T22:50:30Z |
| publishDate | 2009-05-01 |
| publisher | Chinese Anti-Cancer Association; Chinese Antituberculosis Association |
| record_format | Article |
| series | Chinese Journal of Lung Cancer |
| spelling | doaj.art-13c6c69d8b2a482cb2dd47d7a6073be52022-12-22T03:58:36ZzhoChinese Anti-Cancer Association; Chinese Antituberculosis AssociationChinese Journal of Lung Cancer1009-34191999-61872009-05-01125369374Ablation of p120-Catenin Altering the Activity of Small GTPase in Human Lung Cancer CellsNan LIUYang LIUShuli LIUEnhua WANGBackground and objective p120-catenin (p120ctn), a member of the Armadillo gene family, has emerged as an important modulator of small GTPase activities. Therefore, it plays novel roles in tumor malignant phenotype, such as invasion and metastasis, whose mechanism are not well clarified yet. The aim of this study is to explore the roles of p120ctn on the regulation of small GTP family members in lung cancer and the effects to lung cancer invasions andmetastasis. Methods After p120ctn was knocked down by siRNA, in vivo and in vitro analysis was applied to investigate the role and possible mechanism of p120ctn in lung cancer, such as Western Blot, pull-down analysis, and nude mice models. Results p120ctn depletion inactivated RhoA, with the the activity of Cdc42 and Rac1 increased, the invasiveness of lung cancer cells was promoted both in vitro and in vivo . Conclusion p120ctn gene knockdown enhances the metastasis of lung cancer cells, probably by altering expression of small GTPase, such as inactivation of RhoA and activation of Cdc42/Rac1.http://www.lungca.org/index.php?journal=01&page=article&op=view&path%5B%5D=10.3779%2Fj.issn.1009-3419.2009.05.002&path%5B%5D=814p120-cateninSmall GTPaseLung neoplasmsInvasion and metastasis |
| spellingShingle | Nan LIU Yang LIU Shuli LIU Enhua WANG Ablation of p120-Catenin Altering the Activity of Small GTPase in Human Lung Cancer Cells Chinese Journal of Lung Cancer p120-catenin Small GTPase Lung neoplasms Invasion and metastasis |
| title | Ablation of p120-Catenin Altering the Activity of Small GTPase in Human Lung Cancer Cells |
| title_full | Ablation of p120-Catenin Altering the Activity of Small GTPase in Human Lung Cancer Cells |
| title_fullStr | Ablation of p120-Catenin Altering the Activity of Small GTPase in Human Lung Cancer Cells |
| title_full_unstemmed | Ablation of p120-Catenin Altering the Activity of Small GTPase in Human Lung Cancer Cells |
| title_short | Ablation of p120-Catenin Altering the Activity of Small GTPase in Human Lung Cancer Cells |
| title_sort | ablation of p120 catenin altering the activity of small gtpase in human lung cancer cells |
| topic | p120-catenin Small GTPase Lung neoplasms Invasion and metastasis |
| url | http://www.lungca.org/index.php?journal=01&page=article&op=view&path%5B%5D=10.3779%2Fj.issn.1009-3419.2009.05.002&path%5B%5D=814 |
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