Cytotoxicity assessment of beta-lapachone in endothelial cells
The selective activity of an antineoplastic drug is related to its ability to promote cytotoxic action on tumor cells and preserve the integrity of non-neoplastic cells. Beta-lapachone is extracted from the sawdust of Ipe wood, a thick bark tree from the Ipe wood found in the Brazilian Cerrado biom...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Maringá
2021-04-01
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| Series: | Acta Scientiarum: Biological Sciences |
| Subjects: | |
| Online Access: | https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/52784 |
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| author | Patrícia de Almeida Machado Gonçalves Vanessa de Souza Cruz Leandro Lopes Nepomuceno Nayane Peixoto Soares Karla Márcia da Silva Braga Naira Moura Alves Emmanuel Arnhold Eugênio Gonçalves de Araújo |
| author_facet | Patrícia de Almeida Machado Gonçalves Vanessa de Souza Cruz Leandro Lopes Nepomuceno Nayane Peixoto Soares Karla Márcia da Silva Braga Naira Moura Alves Emmanuel Arnhold Eugênio Gonçalves de Araújo |
| author_sort | Patrícia de Almeida Machado Gonçalves |
| collection | DOAJ |
| description |
The selective activity of an antineoplastic drug is related to its ability to promote cytotoxic action on tumor cells and preserve the integrity of non-neoplastic cells. Beta-lapachone is extracted from the sawdust of Ipe wood, a thick bark tree from the Ipe wood found in the Brazilian Cerrado biome. This study aimed to evaluate the cytotoxic action of beta-lapachone in an endothelial cell line. The EA.hy926 cells were seeded in two groups, G1 and G2, cultured and exposed to beta-lapachone at concentrations of 0.0, 0.01, 0.03, 0.1, 0.3, 1 and 3 μM for 24 hours. G1 remained under normal cultivation conditions and G2 was subjected to oxidative stress through an ischemia and reperfusion assay, in a deoxygenated sealed chamber. The cytotoxicity assay was performed using the tetrazolium reduction method. In G1, the cytotoxicity ranged from 0.0 to 10.0%; and in G2 between 0.0 and 6.3%. No statistically significant difference was observed between the obtained values. Moreover, we found no cytotoxic action of beta-lapachone on endothelial cells, and the results point out that the drug might have preserved the cell’s integrity against oxidative stress under the conditions of this experiment. This promising result suggests the possibility of beta-lapachone as a chemotherapy drug with selective activity.
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| first_indexed | 2024-04-13T14:47:39Z |
| format | Article |
| id | doaj.art-13c7482f596d4c579e33de4411193b2b |
| institution | Directory Open Access Journal |
| issn | 1679-9283 1807-863X |
| language | English |
| last_indexed | 2024-04-13T14:47:39Z |
| publishDate | 2021-04-01 |
| publisher | Universidade Estadual de Maringá |
| record_format | Article |
| series | Acta Scientiarum: Biological Sciences |
| spelling | doaj.art-13c7482f596d4c579e33de4411193b2b2022-12-22T02:42:41ZengUniversidade Estadual de MaringáActa Scientiarum: Biological Sciences1679-92831807-863X2021-04-0143110.4025/actascibiolsci.v43i1.5278452784Cytotoxicity assessment of beta-lapachone in endothelial cellsPatrícia de Almeida Machado Gonçalves0Vanessa de Souza Cruz1Leandro Lopes Nepomuceno2Nayane Peixoto Soares3Karla Márcia da Silva Braga4Naira Moura Alves5Emmanuel Arnhold 6Eugênio Gonçalves de Araújo7Universidade Federal de GoiásUniversidade Federal de GoiásUniversidade Federal de GoiásUniversidade Federal de GoiásUniversidade Federal de GoiásUniversidade Federal de GoiásUniversidade Federal de GoiásUniversidade Federal de Goiás The selective activity of an antineoplastic drug is related to its ability to promote cytotoxic action on tumor cells and preserve the integrity of non-neoplastic cells. Beta-lapachone is extracted from the sawdust of Ipe wood, a thick bark tree from the Ipe wood found in the Brazilian Cerrado biome. This study aimed to evaluate the cytotoxic action of beta-lapachone in an endothelial cell line. The EA.hy926 cells were seeded in two groups, G1 and G2, cultured and exposed to beta-lapachone at concentrations of 0.0, 0.01, 0.03, 0.1, 0.3, 1 and 3 μM for 24 hours. G1 remained under normal cultivation conditions and G2 was subjected to oxidative stress through an ischemia and reperfusion assay, in a deoxygenated sealed chamber. The cytotoxicity assay was performed using the tetrazolium reduction method. In G1, the cytotoxicity ranged from 0.0 to 10.0%; and in G2 between 0.0 and 6.3%. No statistically significant difference was observed between the obtained values. Moreover, we found no cytotoxic action of beta-lapachone on endothelial cells, and the results point out that the drug might have preserved the cell’s integrity against oxidative stress under the conditions of this experiment. This promising result suggests the possibility of beta-lapachone as a chemotherapy drug with selective activity. https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/52784antioxidant; cell viability; beta-lapachone; cytotoxicity; Tabebuia. |
| spellingShingle | Patrícia de Almeida Machado Gonçalves Vanessa de Souza Cruz Leandro Lopes Nepomuceno Nayane Peixoto Soares Karla Márcia da Silva Braga Naira Moura Alves Emmanuel Arnhold Eugênio Gonçalves de Araújo Cytotoxicity assessment of beta-lapachone in endothelial cells Acta Scientiarum: Biological Sciences antioxidant; cell viability; beta-lapachone; cytotoxicity; Tabebuia. |
| title | Cytotoxicity assessment of beta-lapachone in endothelial cells |
| title_full | Cytotoxicity assessment of beta-lapachone in endothelial cells |
| title_fullStr | Cytotoxicity assessment of beta-lapachone in endothelial cells |
| title_full_unstemmed | Cytotoxicity assessment of beta-lapachone in endothelial cells |
| title_short | Cytotoxicity assessment of beta-lapachone in endothelial cells |
| title_sort | cytotoxicity assessment of beta lapachone in endothelial cells |
| topic | antioxidant; cell viability; beta-lapachone; cytotoxicity; Tabebuia. |
| url | https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/52784 |
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