Long-Term Administration of LL-37 Can Induce Irreversible Rosacea-like Lesion
Rosacea is a chronic inflammatory skin disease whose late manifestations have not yet been clearly reported in animal models. The objective of this study is to describe the skin lesions and major histopathological changes in a rosacea-like phenotype in mice induced by prolonged LL-37 administration...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-03-01
|
| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/45/4/177 |
| _version_ | 1797605905813995520 |
|---|---|
| author | Chuanxi Zhang Yumeng Kang Ziyan Zhang Heliang Liu Hong Xu Wenchen Cai Xuemin Gao Jie Yang |
| author_facet | Chuanxi Zhang Yumeng Kang Ziyan Zhang Heliang Liu Hong Xu Wenchen Cai Xuemin Gao Jie Yang |
| author_sort | Chuanxi Zhang |
| collection | DOAJ |
| description | Rosacea is a chronic inflammatory skin disease whose late manifestations have not yet been clearly reported in animal models. The objective of this study is to describe the skin lesions and major histopathological changes in a rosacea-like phenotype in mice induced by prolonged LL-37 administration and furthermore, to assess the potential of long-term LL-37 administration in inducing irreversible rosacea-like skin lesion models. Balb/c mice were continuously injected intradermally with LL-37 every 12 h to induce a rosacea-like phenotype. After LL-37 injections were administered for 20 consecutive days, the area of rosacea-like lesions gradually expanded in the first 13 days, then entered a stable phase. Haematoxylin and eosin (H&E) and Van Gieson’s staining showed a high degree of inflammatory cell aggregation, thickening of the epidermis and dermis, and collagen deposition in large quantities. The results of immunofluorescence staining and Western blotting showed that the expression of α-SMA, TNF-α, vimentin, and COL1 in the skin of mice was significantly upregulated. Short-term LL-37 administration induced rosacea-like lesions that only featured the aggregation of inflammatory factors and thickening of the epidermis, whereas no collagen hyperplasia was observed, and a full recovery was noticed. However, rosacea-like skin lesions induced by long-term LL-37 administration did not completely recover. Our study compares rosacea-like lesions induced by short-term versus long-term LL-37 administration, and the results suggest that irreversible rosacea-like lesions can be induced by long-term LL-37 administration. |
| first_indexed | 2024-03-11T05:07:43Z |
| format | Article |
| id | doaj.art-13c8d0ac8cdb4d09ac5ab2f677f4a02f |
| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-11T05:07:43Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-13c8d0ac8cdb4d09ac5ab2f677f4a02f2023-11-17T18:47:22ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452023-03-014542703271610.3390/cimb45040177Long-Term Administration of LL-37 Can Induce Irreversible Rosacea-like LesionChuanxi Zhang0Yumeng Kang1Ziyan Zhang2Heliang Liu3Hong Xu4Wenchen Cai5Xuemin Gao6Jie Yang7Clinical Medical College, North China University of Science and Technology, Tangshan 063210, ChinaClinical Medical College, North China University of Science and Technology, Tangshan 063210, ChinaClinical Medical College, North China University of Science and Technology, Tangshan 063210, ChinaSchool of Public Health, North China University of Science and Technology, Tangshan 063210, ChinaSchool of Public Health, North China University of Science and Technology, Tangshan 063210, ChinaSchool of Public Health, North China University of Science and Technology, Tangshan 063210, ChinaSchool of Public Health, North China University of Science and Technology, Tangshan 063210, ChinaClinical Medical College, North China University of Science and Technology, Tangshan 063210, ChinaRosacea is a chronic inflammatory skin disease whose late manifestations have not yet been clearly reported in animal models. The objective of this study is to describe the skin lesions and major histopathological changes in a rosacea-like phenotype in mice induced by prolonged LL-37 administration and furthermore, to assess the potential of long-term LL-37 administration in inducing irreversible rosacea-like skin lesion models. Balb/c mice were continuously injected intradermally with LL-37 every 12 h to induce a rosacea-like phenotype. After LL-37 injections were administered for 20 consecutive days, the area of rosacea-like lesions gradually expanded in the first 13 days, then entered a stable phase. Haematoxylin and eosin (H&E) and Van Gieson’s staining showed a high degree of inflammatory cell aggregation, thickening of the epidermis and dermis, and collagen deposition in large quantities. The results of immunofluorescence staining and Western blotting showed that the expression of α-SMA, TNF-α, vimentin, and COL1 in the skin of mice was significantly upregulated. Short-term LL-37 administration induced rosacea-like lesions that only featured the aggregation of inflammatory factors and thickening of the epidermis, whereas no collagen hyperplasia was observed, and a full recovery was noticed. However, rosacea-like skin lesions induced by long-term LL-37 administration did not completely recover. Our study compares rosacea-like lesions induced by short-term versus long-term LL-37 administration, and the results suggest that irreversible rosacea-like lesions can be induced by long-term LL-37 administration.https://www.mdpi.com/1467-3045/45/4/177rosaceaLL-37inflammationskin fibrosislesionmodel |
| spellingShingle | Chuanxi Zhang Yumeng Kang Ziyan Zhang Heliang Liu Hong Xu Wenchen Cai Xuemin Gao Jie Yang Long-Term Administration of LL-37 Can Induce Irreversible Rosacea-like Lesion Current Issues in Molecular Biology rosacea LL-37 inflammation skin fibrosis lesion model |
| title | Long-Term Administration of LL-37 Can Induce Irreversible Rosacea-like Lesion |
| title_full | Long-Term Administration of LL-37 Can Induce Irreversible Rosacea-like Lesion |
| title_fullStr | Long-Term Administration of LL-37 Can Induce Irreversible Rosacea-like Lesion |
| title_full_unstemmed | Long-Term Administration of LL-37 Can Induce Irreversible Rosacea-like Lesion |
| title_short | Long-Term Administration of LL-37 Can Induce Irreversible Rosacea-like Lesion |
| title_sort | long term administration of ll 37 can induce irreversible rosacea like lesion |
| topic | rosacea LL-37 inflammation skin fibrosis lesion model |
| url | https://www.mdpi.com/1467-3045/45/4/177 |
| work_keys_str_mv | AT chuanxizhang longtermadministrationofll37caninduceirreversiblerosacealikelesion AT yumengkang longtermadministrationofll37caninduceirreversiblerosacealikelesion AT ziyanzhang longtermadministrationofll37caninduceirreversiblerosacealikelesion AT heliangliu longtermadministrationofll37caninduceirreversiblerosacealikelesion AT hongxu longtermadministrationofll37caninduceirreversiblerosacealikelesion AT wenchencai longtermadministrationofll37caninduceirreversiblerosacealikelesion AT xuemingao longtermadministrationofll37caninduceirreversiblerosacealikelesion AT jieyang longtermadministrationofll37caninduceirreversiblerosacealikelesion |