High Dynamic Range Structured Illumination Microscope Based on Multiple Exposures
Structured illumination microscope (SIM) can double the spatial resolution by using fringed pattern illumination. However, for samples with large intra-scene dynamic ranges, such as clustered objects, SIM fails to reconstruct high-quality images and often exhibits strong artifacts. Herein, we presen...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-03-01
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| Series: | Frontiers in Physics |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fphy.2021.648174/full |
| _version_ | 1818727803814674432 |
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| author | Yong Liang Yong Liang Xiaohu Chen Zhenglong Sun Gang Wen Chong Chen Libo Wang Xin Jin Jie Zhang Guang Yang Jing Gao Hui Li Hui Li Simin Li Simin Li |
| author_facet | Yong Liang Yong Liang Xiaohu Chen Zhenglong Sun Gang Wen Chong Chen Libo Wang Xin Jin Jie Zhang Guang Yang Jing Gao Hui Li Hui Li Simin Li Simin Li |
| author_sort | Yong Liang |
| collection | DOAJ |
| description | Structured illumination microscope (SIM) can double the spatial resolution by using fringed pattern illumination. However, for samples with large intra-scene dynamic ranges, such as clustered objects, SIM fails to reconstruct high-quality images and often exhibits strong artifacts. Herein, we present a high dynamic range SIM (HDR-SIM) method using a multi-exposure acquisition strategy. With HDR-SIM, individual and clustered microspheres and vesicles with 420 times intensity differences can be visualized in the same scene while the delicate structures of the sample were preserved effectively. |
| first_indexed | 2024-12-17T22:19:55Z |
| format | Article |
| id | doaj.art-13cbdf06003f47948099a61897720bc9 |
| institution | Directory Open Access Journal |
| issn | 2296-424X |
| language | English |
| last_indexed | 2024-12-17T22:19:55Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physics |
| spelling | doaj.art-13cbdf06003f47948099a61897720bc92022-12-21T21:30:29ZengFrontiers Media S.A.Frontiers in Physics2296-424X2021-03-01910.3389/fphy.2021.648174648174High Dynamic Range Structured Illumination Microscope Based on Multiple ExposuresYong Liang0Yong Liang1Xiaohu Chen2Zhenglong Sun3Gang Wen4Chong Chen5Libo Wang6Xin Jin7Jie Zhang8Guang Yang9Jing Gao10Hui Li11Hui Li12Simin Li13Simin Li14University of Science and Technology of China, Hefei, ChinaJiangsu Key Laboratory of Medical Optics, CAS Center for Excellence in Molecular Cell Science, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, ChinaJiangsu Key Laboratory of Medical Optics, CAS Center for Excellence in Molecular Cell Science, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, ChinaJiangsu Key Laboratory of Medical Optics, CAS Center for Excellence in Molecular Cell Science, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, ChinaJiangsu Key Laboratory of Medical Optics, CAS Center for Excellence in Molecular Cell Science, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, ChinaUniversity of Science and Technology of China, Hefei, ChinaJiangsu Key Laboratory of Medical Optics, CAS Center for Excellence in Molecular Cell Science, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, ChinaJiangsu Key Laboratory of Medical Optics, CAS Center for Excellence in Molecular Cell Science, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, ChinaJiangsu Key Laboratory of Medical Optics, CAS Center for Excellence in Molecular Cell Science, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, ChinaJiangsu Key Laboratory of Medical Optics, CAS Center for Excellence in Molecular Cell Science, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, ChinaJiangsu Key Laboratory of Medical Optics, CAS Center for Excellence in Molecular Cell Science, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, ChinaUniversity of Science and Technology of China, Hefei, ChinaJiangsu Key Laboratory of Medical Optics, CAS Center for Excellence in Molecular Cell Science, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, ChinaUniversity of Science and Technology of China, Hefei, ChinaJiangsu Key Laboratory of Medical Optics, CAS Center for Excellence in Molecular Cell Science, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, ChinaStructured illumination microscope (SIM) can double the spatial resolution by using fringed pattern illumination. However, for samples with large intra-scene dynamic ranges, such as clustered objects, SIM fails to reconstruct high-quality images and often exhibits strong artifacts. Herein, we present a high dynamic range SIM (HDR-SIM) method using a multi-exposure acquisition strategy. With HDR-SIM, individual and clustered microspheres and vesicles with 420 times intensity differences can be visualized in the same scene while the delicate structures of the sample were preserved effectively.https://www.frontiersin.org/articles/10.3389/fphy.2021.648174/fullsuper-resolutionstructured illumination microscopyhigh dynamic range imagingfusion algorithmmultiple exposures |
| spellingShingle | Yong Liang Yong Liang Xiaohu Chen Zhenglong Sun Gang Wen Chong Chen Libo Wang Xin Jin Jie Zhang Guang Yang Jing Gao Hui Li Hui Li Simin Li Simin Li High Dynamic Range Structured Illumination Microscope Based on Multiple Exposures Frontiers in Physics super-resolution structured illumination microscopy high dynamic range imaging fusion algorithm multiple exposures |
| title | High Dynamic Range Structured Illumination Microscope Based on Multiple Exposures |
| title_full | High Dynamic Range Structured Illumination Microscope Based on Multiple Exposures |
| title_fullStr | High Dynamic Range Structured Illumination Microscope Based on Multiple Exposures |
| title_full_unstemmed | High Dynamic Range Structured Illumination Microscope Based on Multiple Exposures |
| title_short | High Dynamic Range Structured Illumination Microscope Based on Multiple Exposures |
| title_sort | high dynamic range structured illumination microscope based on multiple exposures |
| topic | super-resolution structured illumination microscopy high dynamic range imaging fusion algorithm multiple exposures |
| url | https://www.frontiersin.org/articles/10.3389/fphy.2021.648174/full |
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