Development, Characterization, and In-Vitro Antimicrobial Activity of Lawsonia inermis L. Leaves Hydro-Alcoholic Extract-Based Vaginal Suppositories
Lawsonia inermis (henna) leaves have good antimicrobial, anti-inflammatory and wound healing properties. It is used topically in the form of hamul in gynaecological infections in traditional medicine. However, due to the unpleasant dosage form, it has very low patient compliance. Hence, in this...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2023-01-01
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| Series: | Traditional and Integrative Medicine |
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| Online Access: | https://jtim.tums.ac.ir/index.php/jtim/article/view/701 |
| _version_ | 1827960636919775232 |
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| author | Fakeha Firdous K Khaleequr Rahman Arshiya Sultana Shamsiya Khan |
| author_facet | Fakeha Firdous K Khaleequr Rahman Arshiya Sultana Shamsiya Khan |
| author_sort | Fakeha Firdous K |
| collection | DOAJ |
| description |
Lawsonia inermis (henna) leaves have good antimicrobial, anti-inflammatory
and wound healing properties. It is used topically in the form of hamul in gynaecological
infections in traditional medicine. However, due to the unpleasant dosage form, it has very
low patient compliance. Hence, in this study hydro-alcoholic extract-based vaginal
suppository of henna leaves was formulated and evaluated for various parameters.
Multiple batches of suppository were prepared using polyethylene glycol (PEG)-4000, PEG-400, Tween-80, Span-60, distilled water and hydro-alcoholic extract. Based on the organoleptic character and optimization tests, the final batch was selected. The final batch was evaluated for various physicochemical parameters and antimicrobial activities. Batch containing extract 30%, PEG-4000 37.38%, PEG-400 19%, distilled water 4.55%, Tween-80 and Span 60 4.535% each, passed the disintegration time (11.45mnts), muco-adhesiveness (90°) and melting point (37°C) test and selected as an optimized batch. The mean weight of a suppository was 1.56±0.03g. Secondary plant metabolites in henna leaves and suppository were comparable. Various molecules identified in leaves were also noted in the suppository on GCMS. Heavy metals and microbial contamination were within the permissible limit. Zone of inhibition (at 50µg/ml) and minimum inhibitory concentration for E. coli, S. aureus, S. pyogenes, P. aeruginosa and C. albicans were 20mm, 18mm, 20mm, 16mm, 19mm and 20µg/ml, 10µg/ml, 25µg/ml, 25µg/ml, 45µg/ml respectively. This study indicated satisfactory physicochemical parameters and antimicrobial potential of the hydro-alcoholic extract-based vaginal suppository of henna. Hence, it may be considered as a better alternative to its traditional dosage form.
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| first_indexed | 2024-04-09T16:13:03Z |
| format | Article |
| id | doaj.art-13cce9eb687a46019e38f6427c471621 |
| institution | Directory Open Access Journal |
| issn | 2476-5104 2476-5112 |
| language | English |
| last_indexed | 2024-04-09T16:13:03Z |
| publishDate | 2023-01-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Traditional and Integrative Medicine |
| spelling | doaj.art-13cce9eb687a46019e38f6427c4716212023-04-24T08:08:47ZengTehran University of Medical SciencesTraditional and Integrative Medicine2476-51042476-51122023-01-018110.18502/tim.v8i1.12399Development, Characterization, and In-Vitro Antimicrobial Activity of Lawsonia inermis L. Leaves Hydro-Alcoholic Extract-Based Vaginal SuppositoriesFakeha Firdous K0Khaleequr Rahman1Arshiya Sultana2Shamsiya Khan3Department of Ilmul Saidla (Pharmacy), National Institute of Unani Medicine, Kottigepalya, Magadi Main Road, Bengaluru, IndiaDepartment of Ilmul Saidla (Pharmacy), National Institute of Unani Medicine, Kottigepalya, Magadi Main Road, Bengaluru, IndiaDepartment of Amraze Niswan wa Ilmul Qabalat (Obstetrics and Gyneacology), National Institute of Unani Medicine, Kottigepalya, Magadi Main Road, Bengaluru, IndiaDepartment of Ilmul Saidla (Pharmacy), National Institute of Unani Medicine, Kottigepalya, Magadi Main Road, Bengaluru, India Lawsonia inermis (henna) leaves have good antimicrobial, anti-inflammatory and wound healing properties. It is used topically in the form of hamul in gynaecological infections in traditional medicine. However, due to the unpleasant dosage form, it has very low patient compliance. Hence, in this study hydro-alcoholic extract-based vaginal suppository of henna leaves was formulated and evaluated for various parameters. Multiple batches of suppository were prepared using polyethylene glycol (PEG)-4000, PEG-400, Tween-80, Span-60, distilled water and hydro-alcoholic extract. Based on the organoleptic character and optimization tests, the final batch was selected. The final batch was evaluated for various physicochemical parameters and antimicrobial activities. Batch containing extract 30%, PEG-4000 37.38%, PEG-400 19%, distilled water 4.55%, Tween-80 and Span 60 4.535% each, passed the disintegration time (11.45mnts), muco-adhesiveness (90°) and melting point (37°C) test and selected as an optimized batch. The mean weight of a suppository was 1.56±0.03g. Secondary plant metabolites in henna leaves and suppository were comparable. Various molecules identified in leaves were also noted in the suppository on GCMS. Heavy metals and microbial contamination were within the permissible limit. Zone of inhibition (at 50µg/ml) and minimum inhibitory concentration for E. coli, S. aureus, S. pyogenes, P. aeruginosa and C. albicans were 20mm, 18mm, 20mm, 16mm, 19mm and 20µg/ml, 10µg/ml, 25µg/ml, 25µg/ml, 45µg/ml respectively. This study indicated satisfactory physicochemical parameters and antimicrobial potential of the hydro-alcoholic extract-based vaginal suppository of henna. Hence, it may be considered as a better alternative to its traditional dosage form. https://jtim.tums.ac.ir/index.php/jtim/article/view/701Antimicrobial herbhamulhennaUnani medicineVaginal suppository |
| spellingShingle | Fakeha Firdous K Khaleequr Rahman Arshiya Sultana Shamsiya Khan Development, Characterization, and In-Vitro Antimicrobial Activity of Lawsonia inermis L. Leaves Hydro-Alcoholic Extract-Based Vaginal Suppositories Traditional and Integrative Medicine Antimicrobial herb hamul henna Unani medicine Vaginal suppository |
| title | Development, Characterization, and In-Vitro Antimicrobial Activity of Lawsonia inermis L. Leaves Hydro-Alcoholic Extract-Based Vaginal Suppositories |
| title_full | Development, Characterization, and In-Vitro Antimicrobial Activity of Lawsonia inermis L. Leaves Hydro-Alcoholic Extract-Based Vaginal Suppositories |
| title_fullStr | Development, Characterization, and In-Vitro Antimicrobial Activity of Lawsonia inermis L. Leaves Hydro-Alcoholic Extract-Based Vaginal Suppositories |
| title_full_unstemmed | Development, Characterization, and In-Vitro Antimicrobial Activity of Lawsonia inermis L. Leaves Hydro-Alcoholic Extract-Based Vaginal Suppositories |
| title_short | Development, Characterization, and In-Vitro Antimicrobial Activity of Lawsonia inermis L. Leaves Hydro-Alcoholic Extract-Based Vaginal Suppositories |
| title_sort | development characterization and in vitro antimicrobial activity of lawsonia inermis l leaves hydro alcoholic extract based vaginal suppositories |
| topic | Antimicrobial herb hamul henna Unani medicine Vaginal suppository |
| url | https://jtim.tums.ac.ir/index.php/jtim/article/view/701 |
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