Standardisation and categorization of indigenous microorganisms (IMOs) for inoculated deep litter piggery in India

The present experiment was conducted on standardisation and categorization of Indigenous Microorganisms (IMOs) in India for its future application as inoculum in inoculated deep litter housing of pigs. The cultivation of IMOs was accomplished in four steps, which involved use of half cooked rice, su...

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Main Authors: SEEMA YADAV, P K BHARTI, G K GAUR, BHANITA DEVI, ABHISHEK ABHISHEK, N R SAHOO, RAJESH CHHABRA, ARUN SOMAGOND, MOHIT ANTIL
Format: Article
Language:English
Published: Indian Council of Agricultural Research 2020-09-01
Series:Indian Journal of Animal Sciences
Subjects:
Online Access:https://epubs.icar.org.in/index.php/IJAnS/article/view/104188
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author SEEMA YADAV
P K BHARTI
G K GAUR
BHANITA DEVI
ABHISHEK ABHISHEK
N R SAHOO
RAJESH CHHABRA
ARUN SOMAGOND
MOHIT ANTIL
author_facet SEEMA YADAV
P K BHARTI
G K GAUR
BHANITA DEVI
ABHISHEK ABHISHEK
N R SAHOO
RAJESH CHHABRA
ARUN SOMAGOND
MOHIT ANTIL
author_sort SEEMA YADAV
collection DOAJ
description The present experiment was conducted on standardisation and categorization of Indigenous Microorganisms (IMOs) in India for its future application as inoculum in inoculated deep litter housing of pigs. The cultivation of IMOs was accomplished in four steps, which involved use of half cooked rice, sugar sources, rice bran and soil at 1st, 2nd, 3rd and 4th steps, respectively. The cultivated IMOs at the end of each step from 1st to 4th were named accordingly as IMO-1, IMO-2, IMO-3 and IMO-4. The cultivation of IMOs was done in three groups based on the major sources of energy at 2nd step as control (brown sugar), treatment 1 (Jaggery) and treatment 2 (Molasses). The IMO-1 was obtained after 7 days which was confirmed based on the appearance of white coloured fungal mycelium in all the groups. The IMO-4 was considered as the final product of cultivation process which was confirmed by the presence of fungal mycelium interwoven in the soil. IMO-4 stage was further categorised in different microbial groups based on laboratory examination and only two categories of microbes were witnessed namely bacteria and fungi, none of yeast were found in those inoculums. Out of four types of bacterial colonies, IMB-3 confirmed the presence of Paenibacillus amylolyticus and IMB-4 as Enterococcus casseliflavus. The standardisation of IMOs as inoculum for deep litter housing was performed first time in India.
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spelling doaj.art-13d015cbecea493aa5be618aa21a8c182023-02-23T10:24:37ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272020-09-0190410.56093/ijans.v90i4.104188Standardisation and categorization of indigenous microorganisms (IMOs) for inoculated deep litter piggery in IndiaSEEMA YADAV0P K BHARTI1G K GAUR2BHANITA DEVI3ABHISHEK ABHISHEK4N R SAHOO5RAJESH CHHABRA6ARUN SOMAGOND7MOHIT ANTIL8ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 IndiaICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 IndiaICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 IndiaICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 IndiaICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 IndiaICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 IndiaICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 IndiaICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 IndiaICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 IndiaThe present experiment was conducted on standardisation and categorization of Indigenous Microorganisms (IMOs) in India for its future application as inoculum in inoculated deep litter housing of pigs. The cultivation of IMOs was accomplished in four steps, which involved use of half cooked rice, sugar sources, rice bran and soil at 1st, 2nd, 3rd and 4th steps, respectively. The cultivated IMOs at the end of each step from 1st to 4th were named accordingly as IMO-1, IMO-2, IMO-3 and IMO-4. The cultivation of IMOs was done in three groups based on the major sources of energy at 2nd step as control (brown sugar), treatment 1 (Jaggery) and treatment 2 (Molasses). The IMO-1 was obtained after 7 days which was confirmed based on the appearance of white coloured fungal mycelium in all the groups. The IMO-4 was considered as the final product of cultivation process which was confirmed by the presence of fungal mycelium interwoven in the soil. IMO-4 stage was further categorised in different microbial groups based on laboratory examination and only two categories of microbes were witnessed namely bacteria and fungi, none of yeast were found in those inoculums. Out of four types of bacterial colonies, IMB-3 confirmed the presence of Paenibacillus amylolyticus and IMB-4 as Enterococcus casseliflavus. The standardisation of IMOs as inoculum for deep litter housing was performed first time in India.https://epubs.icar.org.in/index.php/IJAnS/article/view/104188BacteriaFungiIndigenous microorganismsInoculated deep litter housing
spellingShingle SEEMA YADAV
P K BHARTI
G K GAUR
BHANITA DEVI
ABHISHEK ABHISHEK
N R SAHOO
RAJESH CHHABRA
ARUN SOMAGOND
MOHIT ANTIL
Standardisation and categorization of indigenous microorganisms (IMOs) for inoculated deep litter piggery in India
Indian Journal of Animal Sciences
Bacteria
Fungi
Indigenous microorganisms
Inoculated deep litter housing
title Standardisation and categorization of indigenous microorganisms (IMOs) for inoculated deep litter piggery in India
title_full Standardisation and categorization of indigenous microorganisms (IMOs) for inoculated deep litter piggery in India
title_fullStr Standardisation and categorization of indigenous microorganisms (IMOs) for inoculated deep litter piggery in India
title_full_unstemmed Standardisation and categorization of indigenous microorganisms (IMOs) for inoculated deep litter piggery in India
title_short Standardisation and categorization of indigenous microorganisms (IMOs) for inoculated deep litter piggery in India
title_sort standardisation and categorization of indigenous microorganisms imos for inoculated deep litter piggery in india
topic Bacteria
Fungi
Indigenous microorganisms
Inoculated deep litter housing
url https://epubs.icar.org.in/index.php/IJAnS/article/view/104188
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