Overexpression of miR-30b in the developing mouse mammary gland causes a lactation defect and delays involution.
BACKGROUND: MicroRNA (miRNA) are negative regulators of gene expression, capable of exerting pronounced influences upon the translation and stability of mRNA. They are potential regulators of normal mammary gland development and of the maintenance of mammary epithelial progenitor cells. This study w...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3454336?pdf=render |
| _version_ | 1819049469950296064 |
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| author | Sandrine Le Guillou Nezha Sdassi Johann Laubier Bruno Passet Marthe Vilotte Johan Castille Denis Laloë Jacqueline Polyte Stéphan Bouet Florence Jaffrézic Edmond-Paul Cribiu Jean-Luc Vilotte Fabienne Le Provost |
| author_facet | Sandrine Le Guillou Nezha Sdassi Johann Laubier Bruno Passet Marthe Vilotte Johan Castille Denis Laloë Jacqueline Polyte Stéphan Bouet Florence Jaffrézic Edmond-Paul Cribiu Jean-Luc Vilotte Fabienne Le Provost |
| author_sort | Sandrine Le Guillou |
| collection | DOAJ |
| description | BACKGROUND: MicroRNA (miRNA) are negative regulators of gene expression, capable of exerting pronounced influences upon the translation and stability of mRNA. They are potential regulators of normal mammary gland development and of the maintenance of mammary epithelial progenitor cells. This study was undertaken to determine the role of miR-30b on the establishment of a functional mouse mammary gland. miR-30b is a member of the miR-30 family, composed of 6 miRNA that are highly conserved in vertebrates. It has been suggested to play a role in the differentiation of several cell types. METHODOLOGY/PRINCIPAL FINDINGS: The expression of miR-30b was found to be regulated during mammary gland development. Transgenic mice overexpressing miR-30b in mammary epithelial cells were used to investigate its role. During lactation, mammary histological analysis of the transgenic mice showed a reduction in the size of alveolar lumen, a defect of the lipid droplets and a growth defect of pups fed by transgenic females. Moreover some mammary epithelial differentiated structures persisted during involution, suggesting a delay in the process. The genes whose expression was affected by the overexpression of miR-30b were characterized by microarray analysis. CONCLUSION/SIGNIFICANCE: Our data suggests that miR-30b is important for the biology of the mammary gland and demonstrates that the deregulation of only one miRNA could affect lactation and involution. |
| first_indexed | 2024-12-21T11:32:39Z |
| format | Article |
| id | doaj.art-13d4caa906d94b088fcb3d2a57fc3103 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T11:32:39Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-13d4caa906d94b088fcb3d2a57fc31032022-12-21T19:05:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0179e4572710.1371/journal.pone.0045727Overexpression of miR-30b in the developing mouse mammary gland causes a lactation defect and delays involution.Sandrine Le GuillouNezha SdassiJohann LaubierBruno PassetMarthe VilotteJohan CastilleDenis LaloëJacqueline PolyteStéphan BouetFlorence JaffrézicEdmond-Paul CribiuJean-Luc VilotteFabienne Le ProvostBACKGROUND: MicroRNA (miRNA) are negative regulators of gene expression, capable of exerting pronounced influences upon the translation and stability of mRNA. They are potential regulators of normal mammary gland development and of the maintenance of mammary epithelial progenitor cells. This study was undertaken to determine the role of miR-30b on the establishment of a functional mouse mammary gland. miR-30b is a member of the miR-30 family, composed of 6 miRNA that are highly conserved in vertebrates. It has been suggested to play a role in the differentiation of several cell types. METHODOLOGY/PRINCIPAL FINDINGS: The expression of miR-30b was found to be regulated during mammary gland development. Transgenic mice overexpressing miR-30b in mammary epithelial cells were used to investigate its role. During lactation, mammary histological analysis of the transgenic mice showed a reduction in the size of alveolar lumen, a defect of the lipid droplets and a growth defect of pups fed by transgenic females. Moreover some mammary epithelial differentiated structures persisted during involution, suggesting a delay in the process. The genes whose expression was affected by the overexpression of miR-30b were characterized by microarray analysis. CONCLUSION/SIGNIFICANCE: Our data suggests that miR-30b is important for the biology of the mammary gland and demonstrates that the deregulation of only one miRNA could affect lactation and involution.http://europepmc.org/articles/PMC3454336?pdf=render |
| spellingShingle | Sandrine Le Guillou Nezha Sdassi Johann Laubier Bruno Passet Marthe Vilotte Johan Castille Denis Laloë Jacqueline Polyte Stéphan Bouet Florence Jaffrézic Edmond-Paul Cribiu Jean-Luc Vilotte Fabienne Le Provost Overexpression of miR-30b in the developing mouse mammary gland causes a lactation defect and delays involution. PLoS ONE |
| title | Overexpression of miR-30b in the developing mouse mammary gland causes a lactation defect and delays involution. |
| title_full | Overexpression of miR-30b in the developing mouse mammary gland causes a lactation defect and delays involution. |
| title_fullStr | Overexpression of miR-30b in the developing mouse mammary gland causes a lactation defect and delays involution. |
| title_full_unstemmed | Overexpression of miR-30b in the developing mouse mammary gland causes a lactation defect and delays involution. |
| title_short | Overexpression of miR-30b in the developing mouse mammary gland causes a lactation defect and delays involution. |
| title_sort | overexpression of mir 30b in the developing mouse mammary gland causes a lactation defect and delays involution |
| url | http://europepmc.org/articles/PMC3454336?pdf=render |
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