Serum extracellular vesicles containing MIAT induces atrial fibrosis, inflammation and oxidative stress to promote atrial remodeling and atrial fibrillation via blockade of miR‐485‐5p‐mediated CXCL10 inhibition
Abstract Background Atrial fibrillation (AF), a supraventricular arrhythmia that impairs cardiac function, is a main source of morbidity and mortality. Serum‐derived extracellular vesicles (EVs) have been identified to carry potential biomarker or target for the diagnosis and treatment of AF. We int...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-08-01
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| Series: | Clinical and Translational Medicine |
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| Online Access: | https://doi.org/10.1002/ctm2.482 |
| _version_ | 1818715960042848256 |
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| author | Yingwei Chen Xiaojie Chen Haiyu Li Yunpeng Li Dong Cheng Yi Tang Haiqiang Sang |
| author_facet | Yingwei Chen Xiaojie Chen Haiyu Li Yunpeng Li Dong Cheng Yi Tang Haiqiang Sang |
| author_sort | Yingwei Chen |
| collection | DOAJ |
| description | Abstract Background Atrial fibrillation (AF), a supraventricular arrhythmia that impairs cardiac function, is a main source of morbidity and mortality. Serum‐derived extracellular vesicles (EVs) have been identified to carry potential biomarker or target for the diagnosis and treatment of AF. We intended to dissect out the role of lncRNA MIAT enriched in serum‐derived EVs in AF. Methods MIAT expression was quantified in EVs isolated from serum samples of AF patients. Mouse and cell models of AF were developed after angiotensin II (Ang II) induction. Relationship between MIAT, miR‐485‐5p, and CXCL10 was identified. Ectopic expression and depletion assays were implemented in Ang II‐treated mice or HL‐1 cells, or those co‐cultured with serum‐derived EVs to explore the roles of EV‐carried MIAT. Results MIAT was upregulated in EVs from serum samples of AF patients. Further analysis indicated that MIAT enriched in serum‐derived EVs promoted atrial fibrosis, inflammation and oxidative stress, and aggravated the atrial remodeling and resultant AF. Mechanistically, MIAT bound to miR‐485‐5p and weakened its inhibitory role on the target CXCL10, which was responsible for the role of serum‐derived EV containing MIAT in cellular fibrosis, oxidative stress and inflammation, and atrial remodeling in vivo. Conclusions In conclusion, serum‐derived EV containing MIAT facilitates atrial remodeling and exacerbates the AF by abolishing the miR‐485‐5p‐mediated CXCL10 inhibition. This finding aids in the deeper understanding about the pathophysiology of AF. |
| first_indexed | 2024-12-17T19:11:39Z |
| format | Article |
| id | doaj.art-13d9e5627a0d4eeba5381409ba54ad0d |
| institution | Directory Open Access Journal |
| issn | 2001-1326 |
| language | English |
| last_indexed | 2024-12-17T19:11:39Z |
| publishDate | 2021-08-01 |
| publisher | Wiley |
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| series | Clinical and Translational Medicine |
| spelling | doaj.art-13d9e5627a0d4eeba5381409ba54ad0d2022-12-21T21:35:50ZengWileyClinical and Translational Medicine2001-13262021-08-01118n/an/a10.1002/ctm2.482Serum extracellular vesicles containing MIAT induces atrial fibrosis, inflammation and oxidative stress to promote atrial remodeling and atrial fibrillation via blockade of miR‐485‐5p‐mediated CXCL10 inhibitionYingwei Chen0Xiaojie Chen1Haiyu Li2Yunpeng Li3Dong Cheng4Yi Tang5Haiqiang Sang6Department of Cardiology The First Affiliated Hospital of Zhengzhou University Zhengzhou P.R. ChinaDepartment of Cardiology The First Affiliated Hospital of Zhengzhou University Zhengzhou P.R. ChinaDepartment of Cardiology The First Affiliated Hospital of Zhengzhou University Zhengzhou P.R. ChinaDepartment of Cardiology The First Affiliated Hospital of Zhengzhou University Zhengzhou P.R. ChinaDepartment of Cardiology The First Affiliated Hospital of Zhengzhou University Zhengzhou P.R. ChinaDepartment of Cardiology The First Affiliated Hospital of Zhengzhou University Zhengzhou P.R. ChinaDepartment of Cardiology The First Affiliated Hospital of Zhengzhou University Zhengzhou P.R. ChinaAbstract Background Atrial fibrillation (AF), a supraventricular arrhythmia that impairs cardiac function, is a main source of morbidity and mortality. Serum‐derived extracellular vesicles (EVs) have been identified to carry potential biomarker or target for the diagnosis and treatment of AF. We intended to dissect out the role of lncRNA MIAT enriched in serum‐derived EVs in AF. Methods MIAT expression was quantified in EVs isolated from serum samples of AF patients. Mouse and cell models of AF were developed after angiotensin II (Ang II) induction. Relationship between MIAT, miR‐485‐5p, and CXCL10 was identified. Ectopic expression and depletion assays were implemented in Ang II‐treated mice or HL‐1 cells, or those co‐cultured with serum‐derived EVs to explore the roles of EV‐carried MIAT. Results MIAT was upregulated in EVs from serum samples of AF patients. Further analysis indicated that MIAT enriched in serum‐derived EVs promoted atrial fibrosis, inflammation and oxidative stress, and aggravated the atrial remodeling and resultant AF. Mechanistically, MIAT bound to miR‐485‐5p and weakened its inhibitory role on the target CXCL10, which was responsible for the role of serum‐derived EV containing MIAT in cellular fibrosis, oxidative stress and inflammation, and atrial remodeling in vivo. Conclusions In conclusion, serum‐derived EV containing MIAT facilitates atrial remodeling and exacerbates the AF by abolishing the miR‐485‐5p‐mediated CXCL10 inhibition. This finding aids in the deeper understanding about the pathophysiology of AF.https://doi.org/10.1002/ctm2.482atrial fibrillationCXCL10extracellular vesiclesfibrosisinflammationMIAT |
| spellingShingle | Yingwei Chen Xiaojie Chen Haiyu Li Yunpeng Li Dong Cheng Yi Tang Haiqiang Sang Serum extracellular vesicles containing MIAT induces atrial fibrosis, inflammation and oxidative stress to promote atrial remodeling and atrial fibrillation via blockade of miR‐485‐5p‐mediated CXCL10 inhibition Clinical and Translational Medicine atrial fibrillation CXCL10 extracellular vesicles fibrosis inflammation MIAT |
| title | Serum extracellular vesicles containing MIAT induces atrial fibrosis, inflammation and oxidative stress to promote atrial remodeling and atrial fibrillation via blockade of miR‐485‐5p‐mediated CXCL10 inhibition |
| title_full | Serum extracellular vesicles containing MIAT induces atrial fibrosis, inflammation and oxidative stress to promote atrial remodeling and atrial fibrillation via blockade of miR‐485‐5p‐mediated CXCL10 inhibition |
| title_fullStr | Serum extracellular vesicles containing MIAT induces atrial fibrosis, inflammation and oxidative stress to promote atrial remodeling and atrial fibrillation via blockade of miR‐485‐5p‐mediated CXCL10 inhibition |
| title_full_unstemmed | Serum extracellular vesicles containing MIAT induces atrial fibrosis, inflammation and oxidative stress to promote atrial remodeling and atrial fibrillation via blockade of miR‐485‐5p‐mediated CXCL10 inhibition |
| title_short | Serum extracellular vesicles containing MIAT induces atrial fibrosis, inflammation and oxidative stress to promote atrial remodeling and atrial fibrillation via blockade of miR‐485‐5p‐mediated CXCL10 inhibition |
| title_sort | serum extracellular vesicles containing miat induces atrial fibrosis inflammation and oxidative stress to promote atrial remodeling and atrial fibrillation via blockade of mir 485 5p mediated cxcl10 inhibition |
| topic | atrial fibrillation CXCL10 extracellular vesicles fibrosis inflammation MIAT |
| url | https://doi.org/10.1002/ctm2.482 |
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