The Correlation between the Gut Microbiota of <i>Endoclita signifer</i> (Lepidoptera, Hepialidae) Larvae and Their Host Preferences
Insects’ gut microbiota plays a crucial role in their host selection, adaptation, and plasticity. This study explored the impact of gut bacteria on the adaptation of host selection under different stresses (diverse feeding preferences and no feeding preferences). The seventh instar <i>E. signi...
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| Format: | Article |
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MDPI AG
2023-12-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/14/12/919 |
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| author | Jintao Lu Xiaoyan Su Zhende Yang Ping Hu |
| author_facet | Jintao Lu Xiaoyan Su Zhende Yang Ping Hu |
| author_sort | Jintao Lu |
| collection | DOAJ |
| description | Insects’ gut microbiota plays a crucial role in their host selection, adaptation, and plasticity. This study explored the impact of gut bacteria on the adaptation of host selection under different stresses (diverse feeding preferences and no feeding preferences). The seventh instar <i>E. signifer</i> larvae were artificially transferred from the most-selected host <i>E. grandis</i> × <i>E. urophylla</i> (Es) to more preferred hosts, <i>M. apelta</i> (Ma), as well as the non-preferred host, <i>B. papyrifera</i> (Bp). We then obtained the larval gut of three different feeding preference hosts. The gut bacterial DNA was sequenced and analyzed based on 16S rRNA. There were significant differences in the composition of dominant gut bacteria between Es with Ma and Bp, but without significant differences between Ma and Bp. In the process, <i>Burkholderia</i> and <i>Microbacillus</i> with degrading pesticides had significant changes, and <i>Enterococcus</i> with insect gut probiotics also had significant changes. The presence of enterococcus may be one of the main causes of intestinal microbiota changes before and after host transfer. Notably, when the feeding of <i>E. signifer</i> changes, the complex connections that exist between gut bacteria also change. Additionally, there was a negative correlation between the feeding preferences of <i>E. signifer</i> and the metabolic functions of their gut bacteria. This study provided a theoretical basis for the prediction and use of gut bacteria to interfere with the feeding of <i>E. signifer</i>. |
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| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-08T20:39:31Z |
| publishDate | 2023-12-01 |
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| series | Insects |
| spelling | doaj.art-13dc3bf984d14b2c909f82c6a154a7892023-12-22T14:16:13ZengMDPI AGInsects2075-44502023-12-01141291910.3390/insects14120919The Correlation between the Gut Microbiota of <i>Endoclita signifer</i> (Lepidoptera, Hepialidae) Larvae and Their Host PreferencesJintao Lu0Xiaoyan Su1Zhende Yang2Ping Hu3Guangxi Colleges and Universities Key Laboratory for Cultivation and Utilization of Subtropical Forest Plantation, Guangxi Key Laboratory of Forest Ecology and Conservation, College of Forestry, Guangxi University, Nanning 530004, ChinaLiu Wan Forest Farm of Guangxi, Yulin 537899, ChinaGuangxi Colleges and Universities Key Laboratory for Cultivation and Utilization of Subtropical Forest Plantation, Guangxi Key Laboratory of Forest Ecology and Conservation, College of Forestry, Guangxi University, Nanning 530004, ChinaGuangxi Colleges and Universities Key Laboratory for Cultivation and Utilization of Subtropical Forest Plantation, Guangxi Key Laboratory of Forest Ecology and Conservation, College of Forestry, Guangxi University, Nanning 530004, ChinaInsects’ gut microbiota plays a crucial role in their host selection, adaptation, and plasticity. This study explored the impact of gut bacteria on the adaptation of host selection under different stresses (diverse feeding preferences and no feeding preferences). The seventh instar <i>E. signifer</i> larvae were artificially transferred from the most-selected host <i>E. grandis</i> × <i>E. urophylla</i> (Es) to more preferred hosts, <i>M. apelta</i> (Ma), as well as the non-preferred host, <i>B. papyrifera</i> (Bp). We then obtained the larval gut of three different feeding preference hosts. The gut bacterial DNA was sequenced and analyzed based on 16S rRNA. There were significant differences in the composition of dominant gut bacteria between Es with Ma and Bp, but without significant differences between Ma and Bp. In the process, <i>Burkholderia</i> and <i>Microbacillus</i> with degrading pesticides had significant changes, and <i>Enterococcus</i> with insect gut probiotics also had significant changes. The presence of enterococcus may be one of the main causes of intestinal microbiota changes before and after host transfer. Notably, when the feeding of <i>E. signifer</i> changes, the complex connections that exist between gut bacteria also change. Additionally, there was a negative correlation between the feeding preferences of <i>E. signifer</i> and the metabolic functions of their gut bacteria. This study provided a theoretical basis for the prediction and use of gut bacteria to interfere with the feeding of <i>E. signifer</i>.https://www.mdpi.com/2075-4450/14/12/919<i>Endoclita signifer</i>gut microbiotahigh-throughput sequencingtransfer host |
| spellingShingle | Jintao Lu Xiaoyan Su Zhende Yang Ping Hu The Correlation between the Gut Microbiota of <i>Endoclita signifer</i> (Lepidoptera, Hepialidae) Larvae and Their Host Preferences Insects <i>Endoclita signifer</i> gut microbiota high-throughput sequencing transfer host |
| title | The Correlation between the Gut Microbiota of <i>Endoclita signifer</i> (Lepidoptera, Hepialidae) Larvae and Their Host Preferences |
| title_full | The Correlation between the Gut Microbiota of <i>Endoclita signifer</i> (Lepidoptera, Hepialidae) Larvae and Their Host Preferences |
| title_fullStr | The Correlation between the Gut Microbiota of <i>Endoclita signifer</i> (Lepidoptera, Hepialidae) Larvae and Their Host Preferences |
| title_full_unstemmed | The Correlation between the Gut Microbiota of <i>Endoclita signifer</i> (Lepidoptera, Hepialidae) Larvae and Their Host Preferences |
| title_short | The Correlation between the Gut Microbiota of <i>Endoclita signifer</i> (Lepidoptera, Hepialidae) Larvae and Their Host Preferences |
| title_sort | correlation between the gut microbiota of i endoclita signifer i lepidoptera hepialidae larvae and their host preferences |
| topic | <i>Endoclita signifer</i> gut microbiota high-throughput sequencing transfer host |
| url | https://www.mdpi.com/2075-4450/14/12/919 |
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