Does the addition of metformin to carboplatin treatment decreases ovarian reserve damage associated with carboplatin usage?
Abstract Background We aimed to determine whether adding metformin to carboplatin treatment would reduce the damage to ovarian reserve associated with carboplatin use. Methods We included 35 adult female non-pregnant albino Wistar rats approximately three months old, weighing 220–310 g. The rats wer...
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | Journal of Ovarian Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13048-023-01259-2 |
| _version_ | 1797557270292201472 |
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| author | Sevgi Ayhan Necati Hancerliogullari Gurhan Guney Murat Gozukucuk Muzaffer Caydere Sergul Selvi Guney Aytekin Tokmak Yusuf Ustun |
| author_facet | Sevgi Ayhan Necati Hancerliogullari Gurhan Guney Murat Gozukucuk Muzaffer Caydere Sergul Selvi Guney Aytekin Tokmak Yusuf Ustun |
| author_sort | Sevgi Ayhan |
| collection | DOAJ |
| description | Abstract Background We aimed to determine whether adding metformin to carboplatin treatment would reduce the damage to ovarian reserve associated with carboplatin use. Methods We included 35 adult female non-pregnant albino Wistar rats approximately three months old, weighing 220–310 g. The rats were divided into five groups of seven rats according to the treatment they received. Carboplatin and salin was given to Group 2, and carboplatin plus metformin was given to Group 3. Group 4 was administered only metformin. Group 5 was administered only salin. Carboplatin was given to Groups 2 and 3 as a single dose on the 15th day, while metformin was given to Groups 3 and 4 during the 28-day experiment. After oophorectomy, histopathologic analyses of primordial, primary, secondary, and tertiary Graff follicles according to the epithelial cells surrounding the oocyte and total follicular number were conducted per section. Serum Anti-Mullerian Hormone (AMH), tissue catalase, and malonyl dialdehyde levels were measured and compared within each group. Results The baseline and 15th-day serum AMH values of the menstrual cycle were compared among the groups, and no statistically significant differences were observed (p > 0.05). Group 3, which was given both carboplatin and metformin, had statistically significantly higher 28th-day AMH levels than Group 2, which was given only carboplatin and saline (p < 0.001). The number of primordial follicles in Group 3 was found to be statistically significantly higher than in Group 2 (p < 0.001). Tissue catalase enzyme levels in Group 3 were statistically significantly higher than in Group 2 (p < 0.001). Tissue malondialdehyde levels in Group 2 were statistically significantly higher than tissue malondialdehyde levels in Groups 3 and 4 (p < 0.001). Conclusions Metformin may attenuate carboplatin-induced ovarian damage, possibly through its antioxidative effects. |
| first_indexed | 2024-03-10T17:14:52Z |
| format | Article |
| id | doaj.art-13dd376fdcfb4b39a12ddabda7601543 |
| institution | Directory Open Access Journal |
| issn | 1757-2215 |
| language | English |
| last_indexed | 2024-03-10T17:14:52Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Ovarian Research |
| spelling | doaj.art-13dd376fdcfb4b39a12ddabda76015432023-11-20T10:32:29ZengBMCJournal of Ovarian Research1757-22152023-09-011611810.1186/s13048-023-01259-2Does the addition of metformin to carboplatin treatment decreases ovarian reserve damage associated with carboplatin usage?Sevgi Ayhan0Necati Hancerliogullari1Gurhan Guney2Murat Gozukucuk3Muzaffer Caydere4Sergul Selvi Guney5Aytekin Tokmak6Yusuf Ustun7Department of Obstetrics and Gynecology, University of Health Sciences, Bilkent City HospitalDepartment of Obstetrics and Gynecology, University of Health Sciences, Bilkent City HospitalDepartment of Reproductive Endocrinology and Infertility, Balikesir University School of Medicine, Cagis Campus,10145Department of Obstetrics and Gynecology, University of Health Sciences, Ankara Training and Research HospitalDepartment of Pathology, University of Health Sciences, Ankara Training and Research HospitalDepartment of Midwifery, Faculty of Health Sciences, Balikesir UniversityDepartment of Obstetrics and Gynecology, University of Health Sciences, Bilkent City HospitalDepartment of Obstetrics and Gynecology, University of Health Sciences, Ankara Training and Research HospitalAbstract Background We aimed to determine whether adding metformin to carboplatin treatment would reduce the damage to ovarian reserve associated with carboplatin use. Methods We included 35 adult female non-pregnant albino Wistar rats approximately three months old, weighing 220–310 g. The rats were divided into five groups of seven rats according to the treatment they received. Carboplatin and salin was given to Group 2, and carboplatin plus metformin was given to Group 3. Group 4 was administered only metformin. Group 5 was administered only salin. Carboplatin was given to Groups 2 and 3 as a single dose on the 15th day, while metformin was given to Groups 3 and 4 during the 28-day experiment. After oophorectomy, histopathologic analyses of primordial, primary, secondary, and tertiary Graff follicles according to the epithelial cells surrounding the oocyte and total follicular number were conducted per section. Serum Anti-Mullerian Hormone (AMH), tissue catalase, and malonyl dialdehyde levels were measured and compared within each group. Results The baseline and 15th-day serum AMH values of the menstrual cycle were compared among the groups, and no statistically significant differences were observed (p > 0.05). Group 3, which was given both carboplatin and metformin, had statistically significantly higher 28th-day AMH levels than Group 2, which was given only carboplatin and saline (p < 0.001). The number of primordial follicles in Group 3 was found to be statistically significantly higher than in Group 2 (p < 0.001). Tissue catalase enzyme levels in Group 3 were statistically significantly higher than in Group 2 (p < 0.001). Tissue malondialdehyde levels in Group 2 were statistically significantly higher than tissue malondialdehyde levels in Groups 3 and 4 (p < 0.001). Conclusions Metformin may attenuate carboplatin-induced ovarian damage, possibly through its antioxidative effects.https://doi.org/10.1186/s13048-023-01259-2AntioxidantCatalaseMalondialdehydeMetforminCarboplatinOvarian cancer |
| spellingShingle | Sevgi Ayhan Necati Hancerliogullari Gurhan Guney Murat Gozukucuk Muzaffer Caydere Sergul Selvi Guney Aytekin Tokmak Yusuf Ustun Does the addition of metformin to carboplatin treatment decreases ovarian reserve damage associated with carboplatin usage? Journal of Ovarian Research Antioxidant Catalase Malondialdehyde Metformin Carboplatin Ovarian cancer |
| title | Does the addition of metformin to carboplatin treatment decreases ovarian reserve damage associated with carboplatin usage? |
| title_full | Does the addition of metformin to carboplatin treatment decreases ovarian reserve damage associated with carboplatin usage? |
| title_fullStr | Does the addition of metformin to carboplatin treatment decreases ovarian reserve damage associated with carboplatin usage? |
| title_full_unstemmed | Does the addition of metformin to carboplatin treatment decreases ovarian reserve damage associated with carboplatin usage? |
| title_short | Does the addition of metformin to carboplatin treatment decreases ovarian reserve damage associated with carboplatin usage? |
| title_sort | does the addition of metformin to carboplatin treatment decreases ovarian reserve damage associated with carboplatin usage |
| topic | Antioxidant Catalase Malondialdehyde Metformin Carboplatin Ovarian cancer |
| url | https://doi.org/10.1186/s13048-023-01259-2 |
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