Genome-wide identification of bone metastasis-related microRNAs in lung adenocarcinoma by high-throughput sequencing.
BACKGROUND:MicroRNAs (miRNAs) are a class of small noncoding RNAs that regulate gene expression at the post-transcriptional level. They participate in a wide variety of biological processes, including apoptosis, proliferation and metastasis. The aberrant expression of miRNAs has been found to play a...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3620207?pdf=render |
| _version_ | 1818013293918289920 |
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| author | Lin Xie Zuozhang Yang Guoqi Li Lida Shen Xudong Xiang Xuefeng Liu Da Xu Lei Xu Yanjin Chen Zhao Tian Xin Chen |
| author_facet | Lin Xie Zuozhang Yang Guoqi Li Lida Shen Xudong Xiang Xuefeng Liu Da Xu Lei Xu Yanjin Chen Zhao Tian Xin Chen |
| author_sort | Lin Xie |
| collection | DOAJ |
| description | BACKGROUND:MicroRNAs (miRNAs) are a class of small noncoding RNAs that regulate gene expression at the post-transcriptional level. They participate in a wide variety of biological processes, including apoptosis, proliferation and metastasis. The aberrant expression of miRNAs has been found to play an important role in many cancers. RESULTS:To understand the roles of miRNAs in the bone metastasis of lung adenocarcinoma, we constructed two small RNA libraries from blood of lung adenocarcinoma patients with and without bone metastasis. High-throughput sequencing combined with differential expression analysis identified that 7 microRNAs were down-regulated and 21 microRNAs were up-regulated in lung adenocarcinoma with bone metastasis. A total of 797 target genes of the differentially expressed microRNAs were identified using a bioinformatics approach. Functional annotation analysis indicated that a number of pathways might be involved in bone metastasis, survival of the primary origin and metastatic angiogenesis of lung adenocarcinoma. These include the MAPK, Wnt, and NF-kappaB signaling pathways, as well as pathways involving the matrix metalloproteinase, cytoskeletal protein and angiogenesis factors. CONCLUSIONS:This study provides some insights into the molecular mechanisms that underlie lung adenocarcinoma development, thereby aiding the diagnosis and treatment of the disease. |
| first_indexed | 2024-04-14T06:31:22Z |
| format | Article |
| id | doaj.art-13dea14b711e4c58890369f4baf5561e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-14T06:31:22Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-13dea14b711e4c58890369f4baf5561e2022-12-22T02:07:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0184e6121210.1371/journal.pone.0061212Genome-wide identification of bone metastasis-related microRNAs in lung adenocarcinoma by high-throughput sequencing.Lin XieZuozhang YangGuoqi LiLida ShenXudong XiangXuefeng LiuDa XuLei XuYanjin ChenZhao TianXin ChenBACKGROUND:MicroRNAs (miRNAs) are a class of small noncoding RNAs that regulate gene expression at the post-transcriptional level. They participate in a wide variety of biological processes, including apoptosis, proliferation and metastasis. The aberrant expression of miRNAs has been found to play an important role in many cancers. RESULTS:To understand the roles of miRNAs in the bone metastasis of lung adenocarcinoma, we constructed two small RNA libraries from blood of lung adenocarcinoma patients with and without bone metastasis. High-throughput sequencing combined with differential expression analysis identified that 7 microRNAs were down-regulated and 21 microRNAs were up-regulated in lung adenocarcinoma with bone metastasis. A total of 797 target genes of the differentially expressed microRNAs were identified using a bioinformatics approach. Functional annotation analysis indicated that a number of pathways might be involved in bone metastasis, survival of the primary origin and metastatic angiogenesis of lung adenocarcinoma. These include the MAPK, Wnt, and NF-kappaB signaling pathways, as well as pathways involving the matrix metalloproteinase, cytoskeletal protein and angiogenesis factors. CONCLUSIONS:This study provides some insights into the molecular mechanisms that underlie lung adenocarcinoma development, thereby aiding the diagnosis and treatment of the disease.http://europepmc.org/articles/PMC3620207?pdf=render |
| spellingShingle | Lin Xie Zuozhang Yang Guoqi Li Lida Shen Xudong Xiang Xuefeng Liu Da Xu Lei Xu Yanjin Chen Zhao Tian Xin Chen Genome-wide identification of bone metastasis-related microRNAs in lung adenocarcinoma by high-throughput sequencing. PLoS ONE |
| title | Genome-wide identification of bone metastasis-related microRNAs in lung adenocarcinoma by high-throughput sequencing. |
| title_full | Genome-wide identification of bone metastasis-related microRNAs in lung adenocarcinoma by high-throughput sequencing. |
| title_fullStr | Genome-wide identification of bone metastasis-related microRNAs in lung adenocarcinoma by high-throughput sequencing. |
| title_full_unstemmed | Genome-wide identification of bone metastasis-related microRNAs in lung adenocarcinoma by high-throughput sequencing. |
| title_short | Genome-wide identification of bone metastasis-related microRNAs in lung adenocarcinoma by high-throughput sequencing. |
| title_sort | genome wide identification of bone metastasis related micrornas in lung adenocarcinoma by high throughput sequencing |
| url | http://europepmc.org/articles/PMC3620207?pdf=render |
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