Effect of aspartame on biochemical and oxidative stress parameters in rat blood
Aspartame (ASP) is one of the most widely used nonnutritive sweeteners. This study investigates the chronic effects of ASP on hematological and biochemical parameters, and its effects on the oxidative/antioxidative status in the red blood cells of Wistar albino rats. Rats were provided with...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2015-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641500016P.pdf |
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| author | Prokić Marko D. Paunović Milica G. Matić Miloš M. Đorđević Nataša Z. Ognjanović Branka I. Štajn Andraš Š. Saičić Zorica S. |
| author_facet | Prokić Marko D. Paunović Milica G. Matić Miloš M. Đorđević Nataša Z. Ognjanović Branka I. Štajn Andraš Š. Saičić Zorica S. |
| author_sort | Prokić Marko D. |
| collection | DOAJ |
| description | Aspartame (ASP) is one of the most widely used nonnutritive sweeteners. This
study investigates the chronic effects of ASP on hematological and
biochemical parameters, and its effects on the oxidative/antioxidative status
in the red blood cells of Wistar albino rats. Rats were provided with ASP (40
mg/kg/daily for six weeks) in drinking water. Increased food and fluid intake
was observed in the ASP-treated rats. Total body mass was significantly
decreased in the ASP-treated rats. Treatment with ASP caused an increase in
the concentrations of glucose, cholesterol, LDL-cholesterol, and in the
activities of alanine aminotransferase (ALT), aspartate aminotransferase
(AST) and lactate dehydrogenase (LDH), as well as a decrease in the levels of
HDL-cholesterol in the serum. A significant decline in the number of white
blood cells (WBC) was observed after ASP uptake. Based on the results we
conclude that ASP induces oxidative stress, observed as an alteration of the
glutathione redox status, which leads to increased concentrations of nitric
oxide (NO) and lipid peroxides (LPO) in the red blood cells. Changes in
biochemical parameters, lipid metabolism, as well as changes in the levels of
oxidative stress markers and the appearance of signs of liver damage indicate
that chronic use of ASP can lead to the development of hyperglycemia,
hypercholesterolemia and associated diseases. [Projekat Ministarstva nauke
Republike Srbije, br. 173041] |
| first_indexed | 2024-04-11T23:00:12Z |
| format | Article |
| id | doaj.art-13debe43076f44d0ab456ca2de55009f |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-04-11T23:00:12Z |
| publishDate | 2015-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-13debe43076f44d0ab456ca2de55009f2022-12-22T03:58:13ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392015-01-0167253554510.2298/ABS141009016P0354-46641500016PEffect of aspartame on biochemical and oxidative stress parameters in rat bloodProkić Marko D.0Paunović Milica G.1Matić Miloš M.2Đorđević Nataša Z.3Ognjanović Branka I.4Štajn Andraš Š.5Saičić Zorica S.6Institute for Biological Research "Siniša Stanković", Department of Physiology, BelgradeFaculty of Science, Institute of Biology and Ecology, KragujevacFaculty of Science, Institute of Biology and Ecology, KragujevacState University of Novi Pazar, Department of Biomedical Sciences, Novi PazarFaculty of Science, Institute of Biology and Ecology, KragujevacFaculty of Science, Institute of Biology and Ecology, KragujevacInstitute for Biological Research "Siniša Stanković", Department of Physiology, BelgradeAspartame (ASP) is one of the most widely used nonnutritive sweeteners. This study investigates the chronic effects of ASP on hematological and biochemical parameters, and its effects on the oxidative/antioxidative status in the red blood cells of Wistar albino rats. Rats were provided with ASP (40 mg/kg/daily for six weeks) in drinking water. Increased food and fluid intake was observed in the ASP-treated rats. Total body mass was significantly decreased in the ASP-treated rats. Treatment with ASP caused an increase in the concentrations of glucose, cholesterol, LDL-cholesterol, and in the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH), as well as a decrease in the levels of HDL-cholesterol in the serum. A significant decline in the number of white blood cells (WBC) was observed after ASP uptake. Based on the results we conclude that ASP induces oxidative stress, observed as an alteration of the glutathione redox status, which leads to increased concentrations of nitric oxide (NO) and lipid peroxides (LPO) in the red blood cells. Changes in biochemical parameters, lipid metabolism, as well as changes in the levels of oxidative stress markers and the appearance of signs of liver damage indicate that chronic use of ASP can lead to the development of hyperglycemia, hypercholesterolemia and associated diseases. [Projekat Ministarstva nauke Republike Srbije, br. 173041]http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641500016P.pdfAspartamehematological parametersbiochemical parametersoxidative stressappetite |
| spellingShingle | Prokić Marko D. Paunović Milica G. Matić Miloš M. Đorđević Nataša Z. Ognjanović Branka I. Štajn Andraš Š. Saičić Zorica S. Effect of aspartame on biochemical and oxidative stress parameters in rat blood Archives of Biological Sciences Aspartame hematological parameters biochemical parameters oxidative stress appetite |
| title | Effect of aspartame on biochemical and oxidative stress parameters in rat blood |
| title_full | Effect of aspartame on biochemical and oxidative stress parameters in rat blood |
| title_fullStr | Effect of aspartame on biochemical and oxidative stress parameters in rat blood |
| title_full_unstemmed | Effect of aspartame on biochemical and oxidative stress parameters in rat blood |
| title_short | Effect of aspartame on biochemical and oxidative stress parameters in rat blood |
| title_sort | effect of aspartame on biochemical and oxidative stress parameters in rat blood |
| topic | Aspartame hematological parameters biochemical parameters oxidative stress appetite |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641500016P.pdf |
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