<i>Staphylococcus microti</i> Strains Isolated from an Italian Mediterranean Buffalo Herd
<i>S. microti</i> is a new species among non-aureus staphylococci (NAS) frequently found in bovine milk samples and associated with subclinical mastitis (SCM). The aim of this study was to analyze the presence of <i>S. microti</i> in 200 composite milk samples and 104 milking...
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MDPI AG
2023-01-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/1/182 |
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| author | Monica Ambrosio Francesca Paola Nocera Francesca Garofalo Pasquale De Luca Alex Grinberg Luisa De Martino |
| author_facet | Monica Ambrosio Francesca Paola Nocera Francesca Garofalo Pasquale De Luca Alex Grinberg Luisa De Martino |
| author_sort | Monica Ambrosio |
| collection | DOAJ |
| description | <i>S. microti</i> is a new species among non-aureus staphylococci (NAS) frequently found in bovine milk samples and associated with subclinical mastitis (SCM). The aim of this study was to analyze the presence of <i>S. microti</i> in 200 composite milk samples and 104 milking parlor surface swabs collected at a buffalo farm in Southern Italy to define its presence in milk and a milking parlor environment. The samples were inoculated onto different agar plates, and the isolates were identified by MALDI-TOF MS. The strains identified as <i>S. microti</i> (54/304 samples, 17.8%) were collected, and their purified genomic DNA was subjected to PCR amplification and whole 16S rRNA gene sequencing. Furthermore, their phenotypic resistance profiles were evaluated by a disk diffusion method, and the genotypic characterization of the tetracycline resistance was performed for the <i>tet</i>M and <i>tet</i>K genes by multiplex PCR. Four and forty-seven <i>S. microti</i> isolates from milk samples of lactating animals with subclinical mastitis (SCM) and intramammary infection (IMI), respectively, and three isolates from milking parlor surfaces were recovered. The genomic DNA was purified from the bacterial isolates, and the amplification and sequencing of the 16S gene further supported the proteomic identification as <i>S. microti</i>. No clinical mastitis was detected in the herd during the study period. The antimicrobial susceptibility testing revealed a worrisome 100% resistance to tetracyclines, genotypically mediated by the <i>tet</i>M gene for all strains. This study highlights that <i>S. microti</i> may be commonly isolated from dairy buffalo milk and milking parlor equipment. Its association with SCM or IMI remains to be established. |
| first_indexed | 2024-03-11T10:08:34Z |
| format | Article |
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| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T10:08:34Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-13ded2461e8445c7aee72be65133507f2023-11-16T14:49:15ZengMDPI AGAnimals2076-26152023-01-0113118210.3390/ani13010182<i>Staphylococcus microti</i> Strains Isolated from an Italian Mediterranean Buffalo HerdMonica Ambrosio0Francesca Paola Nocera1Francesca Garofalo2Pasquale De Luca3Alex Grinberg4Luisa De Martino5Department of Veterinary Medicine and Animal Production, University of Naples ‘Federico II’, Via Delpino 1, 80137 Naples, ItalyDepartment of Veterinary Medicine and Animal Production, University of Naples ‘Federico II’, Via Delpino 1, 80137 Naples, ItalyIstituto Zooprofilattico Sperimentale del Mezzogiorno, 80055 Portici, ItalyStazione Zoologica Anton Dohrn of Naples, Villa Comunale, 80121 Naples, ItalySchool of Veterinary Science, Massey University, Palmerston North 4442, New ZealandDepartment of Veterinary Medicine and Animal Production, University of Naples ‘Federico II’, Via Delpino 1, 80137 Naples, Italy<i>S. microti</i> is a new species among non-aureus staphylococci (NAS) frequently found in bovine milk samples and associated with subclinical mastitis (SCM). The aim of this study was to analyze the presence of <i>S. microti</i> in 200 composite milk samples and 104 milking parlor surface swabs collected at a buffalo farm in Southern Italy to define its presence in milk and a milking parlor environment. The samples were inoculated onto different agar plates, and the isolates were identified by MALDI-TOF MS. The strains identified as <i>S. microti</i> (54/304 samples, 17.8%) were collected, and their purified genomic DNA was subjected to PCR amplification and whole 16S rRNA gene sequencing. Furthermore, their phenotypic resistance profiles were evaluated by a disk diffusion method, and the genotypic characterization of the tetracycline resistance was performed for the <i>tet</i>M and <i>tet</i>K genes by multiplex PCR. Four and forty-seven <i>S. microti</i> isolates from milk samples of lactating animals with subclinical mastitis (SCM) and intramammary infection (IMI), respectively, and three isolates from milking parlor surfaces were recovered. The genomic DNA was purified from the bacterial isolates, and the amplification and sequencing of the 16S gene further supported the proteomic identification as <i>S. microti</i>. No clinical mastitis was detected in the herd during the study period. The antimicrobial susceptibility testing revealed a worrisome 100% resistance to tetracyclines, genotypically mediated by the <i>tet</i>M gene for all strains. This study highlights that <i>S. microti</i> may be commonly isolated from dairy buffalo milk and milking parlor equipment. Its association with SCM or IMI remains to be established.https://www.mdpi.com/2076-2615/13/1/182<i>Staphylococcus microti</i>buffalo milksubclinical mastitisintramammary infectionmilking parlor surfaces |
| spellingShingle | Monica Ambrosio Francesca Paola Nocera Francesca Garofalo Pasquale De Luca Alex Grinberg Luisa De Martino <i>Staphylococcus microti</i> Strains Isolated from an Italian Mediterranean Buffalo Herd Animals <i>Staphylococcus microti</i> buffalo milk subclinical mastitis intramammary infection milking parlor surfaces |
| title | <i>Staphylococcus microti</i> Strains Isolated from an Italian Mediterranean Buffalo Herd |
| title_full | <i>Staphylococcus microti</i> Strains Isolated from an Italian Mediterranean Buffalo Herd |
| title_fullStr | <i>Staphylococcus microti</i> Strains Isolated from an Italian Mediterranean Buffalo Herd |
| title_full_unstemmed | <i>Staphylococcus microti</i> Strains Isolated from an Italian Mediterranean Buffalo Herd |
| title_short | <i>Staphylococcus microti</i> Strains Isolated from an Italian Mediterranean Buffalo Herd |
| title_sort | i staphylococcus microti i strains isolated from an italian mediterranean buffalo herd |
| topic | <i>Staphylococcus microti</i> buffalo milk subclinical mastitis intramammary infection milking parlor surfaces |
| url | https://www.mdpi.com/2076-2615/13/1/182 |
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