Monitoring In Vitro Extracellular Matrix Protein Conformations in the Presence of Biomimetic Bone-Regeneration Scaffolds Using Functionalized Gold-Edge-Coated Triangular Silver Nanoparticles
In the cellular environment, high noise levels, such as fluctuations in biochemical reactions, protein variability, molecular diffusion, cell-to-cell contact, and pH, can both mediate and interfere with cellular functions. In this work, gold edge-coated triangular silver nanoparticles (AuTSNP) were...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/13/1/57 |
| _version_ | 1797431330605105152 |
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| author | Laura G. Rodriguez Barroso Farah Alwani Azaman Robert Pogue Declan Devine Margaret Brennan Fournet |
| author_facet | Laura G. Rodriguez Barroso Farah Alwani Azaman Robert Pogue Declan Devine Margaret Brennan Fournet |
| author_sort | Laura G. Rodriguez Barroso |
| collection | DOAJ |
| description | In the cellular environment, high noise levels, such as fluctuations in biochemical reactions, protein variability, molecular diffusion, cell-to-cell contact, and pH, can both mediate and interfere with cellular functions. In this work, gold edge-coated triangular silver nanoparticles (AuTSNP) were validated as a promising new tool to indicate protein conformational transitions in cultured cells and to monitor essential protein activity in the presence of an optimized bone biomimetic chitosan-based scaffold whose rational design mimics the ECM as a natural scaffold. A chitosan-based scaffold formulation with hydroxyapatite (CS/HAp) was selected due to its promising features for orthopedic applications, including combined high mechanical strength biocompatibility and biodegradability. Functionalized AuTSNP-based tests with the model ECM protein, fibronectin (Fn), illustrate that the protein interactions can be clearly sensed over time through the local surface plasmon resonance (LSPR) technique. This demonstrates that AuTNSP are a powerful tool to detect protein conformational activity in the presence of biomimetic bone tissue regeneration scaffolds within a cellular environment that comprises a diversity of molecular cues. |
| first_indexed | 2024-03-09T09:43:21Z |
| format | Article |
| id | doaj.art-13dfe47e6b7544d896c9457b41cddf46 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T09:43:21Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-13dfe47e6b7544d896c9457b41cddf462023-12-02T00:44:04ZengMDPI AGNanomaterials2079-49912022-12-011315710.3390/nano13010057Monitoring In Vitro Extracellular Matrix Protein Conformations in the Presence of Biomimetic Bone-Regeneration Scaffolds Using Functionalized Gold-Edge-Coated Triangular Silver NanoparticlesLaura G. Rodriguez Barroso0Farah Alwani Azaman1Robert Pogue2Declan Devine3Margaret Brennan Fournet4Technological University of the Shannon: Midlands Midwest, Dublin Rd., N37 HD68 Athlone, Co. Westmeath, IrelandTechnological University of the Shannon: Midlands Midwest, Dublin Rd., N37 HD68 Athlone, Co. Westmeath, IrelandTechnological University of the Shannon: Midlands Midwest, Dublin Rd., N37 HD68 Athlone, Co. Westmeath, IrelandTechnological University of the Shannon: Midlands Midwest, Dublin Rd., N37 HD68 Athlone, Co. Westmeath, IrelandTechnological University of the Shannon: Midlands Midwest, Dublin Rd., N37 HD68 Athlone, Co. Westmeath, IrelandIn the cellular environment, high noise levels, such as fluctuations in biochemical reactions, protein variability, molecular diffusion, cell-to-cell contact, and pH, can both mediate and interfere with cellular functions. In this work, gold edge-coated triangular silver nanoparticles (AuTSNP) were validated as a promising new tool to indicate protein conformational transitions in cultured cells and to monitor essential protein activity in the presence of an optimized bone biomimetic chitosan-based scaffold whose rational design mimics the ECM as a natural scaffold. A chitosan-based scaffold formulation with hydroxyapatite (CS/HAp) was selected due to its promising features for orthopedic applications, including combined high mechanical strength biocompatibility and biodegradability. Functionalized AuTSNP-based tests with the model ECM protein, fibronectin (Fn), illustrate that the protein interactions can be clearly sensed over time through the local surface plasmon resonance (LSPR) technique. This demonstrates that AuTNSP are a powerful tool to detect protein conformational activity in the presence of biomimetic bone tissue regeneration scaffolds within a cellular environment that comprises a diversity of molecular cues.https://www.mdpi.com/2079-4991/13/1/57triangular silver nanoparticlesfibronectinLSPRextracellular matrixregeneration scaffold |
| spellingShingle | Laura G. Rodriguez Barroso Farah Alwani Azaman Robert Pogue Declan Devine Margaret Brennan Fournet Monitoring In Vitro Extracellular Matrix Protein Conformations in the Presence of Biomimetic Bone-Regeneration Scaffolds Using Functionalized Gold-Edge-Coated Triangular Silver Nanoparticles Nanomaterials triangular silver nanoparticles fibronectin LSPR extracellular matrix regeneration scaffold |
| title | Monitoring In Vitro Extracellular Matrix Protein Conformations in the Presence of Biomimetic Bone-Regeneration Scaffolds Using Functionalized Gold-Edge-Coated Triangular Silver Nanoparticles |
| title_full | Monitoring In Vitro Extracellular Matrix Protein Conformations in the Presence of Biomimetic Bone-Regeneration Scaffolds Using Functionalized Gold-Edge-Coated Triangular Silver Nanoparticles |
| title_fullStr | Monitoring In Vitro Extracellular Matrix Protein Conformations in the Presence of Biomimetic Bone-Regeneration Scaffolds Using Functionalized Gold-Edge-Coated Triangular Silver Nanoparticles |
| title_full_unstemmed | Monitoring In Vitro Extracellular Matrix Protein Conformations in the Presence of Biomimetic Bone-Regeneration Scaffolds Using Functionalized Gold-Edge-Coated Triangular Silver Nanoparticles |
| title_short | Monitoring In Vitro Extracellular Matrix Protein Conformations in the Presence of Biomimetic Bone-Regeneration Scaffolds Using Functionalized Gold-Edge-Coated Triangular Silver Nanoparticles |
| title_sort | monitoring in vitro extracellular matrix protein conformations in the presence of biomimetic bone regeneration scaffolds using functionalized gold edge coated triangular silver nanoparticles |
| topic | triangular silver nanoparticles fibronectin LSPR extracellular matrix regeneration scaffold |
| url | https://www.mdpi.com/2079-4991/13/1/57 |
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