Transferability of heterologous microsatellite primers in Leiarius marmoratus
The Amazonian Jundiá (Leiarius marmoratus) (Siluliformes: Pimelodidae) is a species of catfish with social and economic importance in some South American countries such as Brazil and Colombia. Genetic evaluation of this species is limited due to the lack of specific molecular markers, hindering stud...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Londrina
2020-08-01
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| Series: | Semina: Ciências Agrárias |
| Subjects: | |
| Online Access: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38246 |
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| author | Victor César Freitas Pandolfi Andrei Lincoln Yamachita Felipe Pinheiro de Souza Ed Christian Suzuki de Lima Denise Rocha Ayres Annaiza Braga Bignardi Jayme Aparecido Povh Ulisses de Pádua Pereira Angela Maria Urrea-Rojas Nelson Mauricio Lopera-Barrero |
| author_facet | Victor César Freitas Pandolfi Andrei Lincoln Yamachita Felipe Pinheiro de Souza Ed Christian Suzuki de Lima Denise Rocha Ayres Annaiza Braga Bignardi Jayme Aparecido Povh Ulisses de Pádua Pereira Angela Maria Urrea-Rojas Nelson Mauricio Lopera-Barrero |
| author_sort | Victor César Freitas Pandolfi |
| collection | DOAJ |
| description | The Amazonian Jundiá (Leiarius marmoratus) (Siluliformes: Pimelodidae) is a species of catfish with social and economic importance in some South American countries such as Brazil and Colombia. Genetic evaluation of this species is limited due to the lack of specific molecular markers, hindering studies on genetic diversity and structure in animals under captive conditions or in natural populations. The objective of the present study was to evaluate the transferability of heterologous microsatellite markers in Leiarius marmoratus. Thirty-two heterologous primers were tested in L. marmoratus. The primers that presented the best standards were applied to 20 specimens, and the number of alleles (Na), number of effective alleles (Ne), gene diversity per Locus (GdL) and percentage of amplification failure (Md) were calculated. Eleven primers demonstrated satisfactory transferability patterns, all from the fish of the Pimelodidae family, of which, seven were monomorphic and four polymorphic. The eleven markers presented low percentage of Md (mean was 5.9% samples per locus). Na varied from one to two alleles per locus, revealing low polymorphism in the evaluated samples. The mean Ne and GdL numbers were 1.77 and 0.32, respectively. The transferability of the heterologous microsatellite loci in L. marmoratus was shown to be possible. However, further tests are needed to apply these markers in population genetic studies. |
| first_indexed | 2024-04-10T20:36:48Z |
| format | Article |
| id | doaj.art-13e19629ac7248ccb68387476868487f |
| institution | Directory Open Access Journal |
| issn | 1676-546X 1679-0359 |
| language | English |
| last_indexed | 2024-04-10T20:36:48Z |
| publishDate | 2020-08-01 |
| publisher | Universidade Estadual de Londrina |
| record_format | Article |
| series | Semina: Ciências Agrárias |
| spelling | doaj.art-13e19629ac7248ccb68387476868487f2023-01-24T20:30:19ZengUniversidade Estadual de LondrinaSemina: Ciências Agrárias1676-546X1679-03592020-08-01415supl110.5433/1679-0359.2020v41n5supl1p2297Transferability of heterologous microsatellite primers in Leiarius marmoratusVictor César Freitas Pandolfi0Andrei Lincoln Yamachita1Felipe Pinheiro de Souza2Ed Christian Suzuki de Lima3Denise Rocha Ayres4Annaiza Braga Bignardi5Jayme Aparecido Povh6Ulisses de Pádua Pereira7Angela Maria Urrea-Rojas8Nelson Mauricio Lopera-Barrero9Universidade Estadual de LondrinaUniversidade Estadual de LondrinaUniversidade Estadual de LondrinaUniversidade Estadual de LondrinaFederal University of Mato GrossoFederal University of Mato GrossoUniversidade Federal de Mato Grosso do SulUniversidade Estadual de LondrinaUniversidade Estadual de LondrinaUniversidade Estadual de LondrinaThe Amazonian Jundiá (Leiarius marmoratus) (Siluliformes: Pimelodidae) is a species of catfish with social and economic importance in some South American countries such as Brazil and Colombia. Genetic evaluation of this species is limited due to the lack of specific molecular markers, hindering studies on genetic diversity and structure in animals under captive conditions or in natural populations. The objective of the present study was to evaluate the transferability of heterologous microsatellite markers in Leiarius marmoratus. Thirty-two heterologous primers were tested in L. marmoratus. The primers that presented the best standards were applied to 20 specimens, and the number of alleles (Na), number of effective alleles (Ne), gene diversity per Locus (GdL) and percentage of amplification failure (Md) were calculated. Eleven primers demonstrated satisfactory transferability patterns, all from the fish of the Pimelodidae family, of which, seven were monomorphic and four polymorphic. The eleven markers presented low percentage of Md (mean was 5.9% samples per locus). Na varied from one to two alleles per locus, revealing low polymorphism in the evaluated samples. The mean Ne and GdL numbers were 1.77 and 0.32, respectively. The transferability of the heterologous microsatellite loci in L. marmoratus was shown to be possible. However, further tests are needed to apply these markers in population genetic studies.https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38246AquacultureAmazonian JundiáCross-amplificationGenetic conservationSSR. |
| spellingShingle | Victor César Freitas Pandolfi Andrei Lincoln Yamachita Felipe Pinheiro de Souza Ed Christian Suzuki de Lima Denise Rocha Ayres Annaiza Braga Bignardi Jayme Aparecido Povh Ulisses de Pádua Pereira Angela Maria Urrea-Rojas Nelson Mauricio Lopera-Barrero Transferability of heterologous microsatellite primers in Leiarius marmoratus Semina: Ciências Agrárias Aquaculture Amazonian Jundiá Cross-amplification Genetic conservation SSR. |
| title | Transferability of heterologous microsatellite primers in Leiarius marmoratus |
| title_full | Transferability of heterologous microsatellite primers in Leiarius marmoratus |
| title_fullStr | Transferability of heterologous microsatellite primers in Leiarius marmoratus |
| title_full_unstemmed | Transferability of heterologous microsatellite primers in Leiarius marmoratus |
| title_short | Transferability of heterologous microsatellite primers in Leiarius marmoratus |
| title_sort | transferability of heterologous microsatellite primers in leiarius marmoratus |
| topic | Aquaculture Amazonian Jundiá Cross-amplification Genetic conservation SSR. |
| url | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38246 |
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