Difference in cytological findings of healthy and conjunctivitis/keratoconjunctivitis affected canine eyes between variably experienced evaluators
Background and Aim: Cytology investigations are a frequent part of ophthalmological examination. We aimed to assess whether the cytological findings of healthy and conjunctivitis/keratoconjunctivitis samples differed based on the evaluator's experience. Materials and Methods: A study evaluat...
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| Format: | Article |
| Language: | English |
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Veterinary World
2022-07-01
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| Series: | Veterinary World |
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| Online Access: | http://www.veterinaryworld.org/Vol.15/July-2022/33.pdf |
| _version_ | 1818002357556871168 |
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| author | Inese Berzina Anastasija Terentjeva Liga Kovalcuka |
| author_facet | Inese Berzina Anastasija Terentjeva Liga Kovalcuka |
| author_sort | Inese Berzina |
| collection | DOAJ |
| description | Background and Aim: Cytology investigations are a frequent part of ophthalmological examination. We aimed to assess whether the cytological findings of healthy and conjunctivitis/keratoconjunctivitis samples differed based on the evaluator's experience.
Materials and Methods: A study evaluated healthy eyes (n = 40) and eyes affected with keratoconjunctivitis and/ or conjunctivitis (n = 28) in dogs. An ophthalmological examination was performed before sampling the eyes using a sterile cotton swab. An evaluator with theoretical experience and one with undergone clinical pathology residency training performed cytology blinded to the clinical findings.
Results: In the healthy eyes group, the agreement between the evaluators for cellularity was nonexistent, while that for cell preservation and mucus content was fair. In the affected eyes group, the agreement for cellularity and mucus content was moderate, while that for cell preservation was fair. The inadequate sample rate differed significantly between the two evaluators in the healthy eyes group (p = 0.006) but not in the affected eyes group (p = 0.083). Bacterial presence was detected by both evaluators, and the findings did not differ statistically from the bacteriology results (p = 0.05). Significant variations were noted in the differential cell count; the mean count of the superficial epithelial cells and goblet cells of the healthy eyes group (p < 0.05) and that of the basal/intermediate cells and neutrophils of the affected eyes (p < 0.05) showed significant differences.
Conclusion: The evaluator's experience significantly affected the differential cell count in both the healthy and affected eyes groups. Neutrophil degeneration was not observed by the less experienced evaluator, whereas bacteria were detected equally well by both the evaluators. |
| first_indexed | 2024-04-14T03:45:46Z |
| format | Article |
| id | doaj.art-13e46ca828cd45c1b8ae7c52a793c5e0 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-04-14T03:45:46Z |
| publishDate | 2022-07-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-13e46ca828cd45c1b8ae7c52a793c5e02022-12-22T02:14:17ZengVeterinary WorldVeterinary World0972-89882231-09162022-07-011571852185610.14202/vetworld.2022.1852-1856Difference in cytological findings of healthy and conjunctivitis/keratoconjunctivitis affected canine eyes between variably experienced evaluatorsInese Berzina0https://orcid.org/0000-0002-9096-3542Anastasija Terentjeva1https://orcid.org/0000-0002-2474-742XLiga Kovalcuka2https://orcid.org/0000-0002-2524-7427Preclinical Institute, Faculty of Veterinary Medicine, Latvia University of Life Sciences and Technologies, Jelgava, Latvia.Clinical Institute, Faculty of Veterinary Medicine, Latvia University of Life Sciences and Technologies, Jelgava, Latvia.Clinical Institute, Faculty of Veterinary Medicine, Latvia University of Life Sciences and Technologies, Jelgava, Latvia.Background and Aim: Cytology investigations are a frequent part of ophthalmological examination. We aimed to assess whether the cytological findings of healthy and conjunctivitis/keratoconjunctivitis samples differed based on the evaluator's experience. Materials and Methods: A study evaluated healthy eyes (n = 40) and eyes affected with keratoconjunctivitis and/ or conjunctivitis (n = 28) in dogs. An ophthalmological examination was performed before sampling the eyes using a sterile cotton swab. An evaluator with theoretical experience and one with undergone clinical pathology residency training performed cytology blinded to the clinical findings. Results: In the healthy eyes group, the agreement between the evaluators for cellularity was nonexistent, while that for cell preservation and mucus content was fair. In the affected eyes group, the agreement for cellularity and mucus content was moderate, while that for cell preservation was fair. The inadequate sample rate differed significantly between the two evaluators in the healthy eyes group (p = 0.006) but not in the affected eyes group (p = 0.083). Bacterial presence was detected by both evaluators, and the findings did not differ statistically from the bacteriology results (p = 0.05). Significant variations were noted in the differential cell count; the mean count of the superficial epithelial cells and goblet cells of the healthy eyes group (p < 0.05) and that of the basal/intermediate cells and neutrophils of the affected eyes (p < 0.05) showed significant differences. Conclusion: The evaluator's experience significantly affected the differential cell count in both the healthy and affected eyes groups. Neutrophil degeneration was not observed by the less experienced evaluator, whereas bacteria were detected equally well by both the evaluators.http://www.veterinaryworld.org/Vol.15/July-2022/33.pdfconjunctival cytologymicroscopy experienceneutrophil morphology |
| spellingShingle | Inese Berzina Anastasija Terentjeva Liga Kovalcuka Difference in cytological findings of healthy and conjunctivitis/keratoconjunctivitis affected canine eyes between variably experienced evaluators Veterinary World conjunctival cytology microscopy experience neutrophil morphology |
| title | Difference in cytological findings of healthy and conjunctivitis/keratoconjunctivitis affected canine eyes between variably experienced evaluators |
| title_full | Difference in cytological findings of healthy and conjunctivitis/keratoconjunctivitis affected canine eyes between variably experienced evaluators |
| title_fullStr | Difference in cytological findings of healthy and conjunctivitis/keratoconjunctivitis affected canine eyes between variably experienced evaluators |
| title_full_unstemmed | Difference in cytological findings of healthy and conjunctivitis/keratoconjunctivitis affected canine eyes between variably experienced evaluators |
| title_short | Difference in cytological findings of healthy and conjunctivitis/keratoconjunctivitis affected canine eyes between variably experienced evaluators |
| title_sort | difference in cytological findings of healthy and conjunctivitis keratoconjunctivitis affected canine eyes between variably experienced evaluators |
| topic | conjunctival cytology microscopy experience neutrophil morphology |
| url | http://www.veterinaryworld.org/Vol.15/July-2022/33.pdf |
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